Superovulatory response and embryo quality of Holstein heifers treated with one or two injections of somatotropin

Authors

  • Juan González Maldonado
  • Raymundo Rangel Santos
  • Raymundo Rodríguez de Lara

Keywords:

conception rate, donor heifer, incidence of estrus, preovulatory follicle

Abstract


Background: recombinant bovine somatotropin (rbST) is used in bovine embryo donors to improve superovulatory response and embryo quality. Objective: this study evaluated the effect of applying one versus two injections of 500 mg of rbST to donor Holstein heifers on estrus incidence (IE), diameter of the largest preovulatory follicle (dLPF), superovulation response (SR), embryo yield, and pregnancy rate in recipient Holstein cows (PRR). Methods: two superstimulation programs were conducted. Heifers were assigned to one of two treatments: 1) rbST-I, n = 5: heifers received one injection of rbST on day 0 (day of CIDR (controlled internal drug release) removal); 2) rbST-II, n = 5: heifers received the first rbST injection on day -8 and the second one on day 0. Thirty-eight cows were used as recipients and were assigned to receive one embryo from one of the two treatments. Results: there was no effect of treatment (p > 0.05) on PRR, dLPF, IE, and SR. The number of oocytes increased (p < 0.05) in the rbST-I treatment (1.3 ± 0.4 vs 0.2 ± 0.2), but there was no difference in the number (p > 0.05) of degenerated (1.0 ± 0.5 vs 4.5 ± 3.0) or transferable (1.0 ± 0.5 vs 1.4 ± 0.7) embryos between heifers in the rbST-I and rbST-II treatments, respectively. Moreover, no oocytes or embryos were recovered from 36.8% of donor heifers in either treatment. Conclusion: the application of 500 mg of rbST on days -8 and 0 of the follicular wave synchronization program did not increase the superovulatory response but significantly reduced the number of oocytes recovered from superovulated Holstein heifers.

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References

Adamiak SJ, Mackie K, Watt RG, Webb R, Sinclair KD. Impact of nutrition on oocyte quality: cumulative effects of body composition and diet leading to hyperinsulinemia in cattle. Biol of Reprod 2005; 73:918-926.Ahmad-Thabet SM. The fimbrio-ovarian relation and its role on ovum picking in unexplained infertility: the fimbrio-ovarian accessibility tests. J Obst Gynaecol Res 2000; 26:65-70.Bilby TR, Guzeloglu A. Kamimura S, Pancarci SM, Michel F, Head HH, Thatcher WW. Pregnancy and bovine somatotropin in nonlactating dairy cows: I. Ovarian, conceptus, and insulin-like growth factor system responses. J Dairy Sci 2004; 87:3256-3267.Borges AM, Torres CAA, Ruas JRM, Rocha-Júnior VR, Carvalho GR. Resposta superovulatória de novilhas mestiças holandês-zebu tratadas com somatotropina bovina recombinante (rbST). Rev Bras Zootec 2001; 30:1439-1444.Canadian council on animal care. CCAC guidelines on: the care and use of farm animals in research, teaching and testing. Canadian council on animal care, Ottawa, Canada. 2009.Chi MM, Schlein AL, Moley KH. High insulin-like growth factor 1 (IGF-1) and insulin concentrations trigger apoptosis in the mouse blastocyst via down-regulation of the IGF-I receptor. Endocrinology 2000;141:4784-4792.

Rev Colomb Cienc Pecu 2015; 28:339-346González-Maldonado J et al. Somatotropin and superovulation in cowsChupin D, Saumande J. New attempts to decrease the variability of ovarian response to PMSG in cattle. Ann Biol Anim Biochm Biophys 1979; 19:1489-1498.De la Sota RL, Lucy MC, Staples CR, Thatcher WW. Effects of recombinant bovine somatotropin (Sometribove) on ovarian function in lactating and nonlactating dairy cows. J Dairy Sci 1993; 76:1002-1013.García E. Modificaciones del Sistema de Clasificación Climática de Köppen. Instituto de Geografía, Universidad Nacional Autónoma de México, México; 1988.Gong JG, Bramley TA, Webb R. The effect of recombinant bovine somatotrophin on ovarian follicular growth and development in heifers. J Reprod and Fertil 1993a; 97:247-254. Gong JG, Bramley TA, Wilmut I, Webb R. Effect of recombinant bovine somatotropin on the superovulatory response to pregnant mare serum gonadotropin in heifers. Biol Reprod 1993b; 48:1141-1149.Hayakawa H, Hirai T, Takimoto A, Ideta A, Aoyagi Y. Superovulation and embryo transfer in Holstein cattle using sexed sperm. Theriogenology 2009; 71:68-73.Herrier A, Einspanier R, Schams D, Niemann H. Effect of recombinant bovine somatotropin (rBST) on follicular IGF-I contents and the ovarian response following superovulatory treatment in dairy cows: a preliminary study. Theriogenology 1994; 41:601-611.Ireland JJ, Ward F, Jimenez-Krassel F, Ireland JL, Smith GW, Lonergan P, Evans AC. Follicle numbers are highly repeatable within individual animals but are inversely correlated with FSH concentrations and the proportion of good-quality embryos after ovarian stimulation in cattle. Hum Reprod 2007; 22:1687-1695.Katagiri S, Moon YS, Yuen BH. The role for the uterine insulin-like growth factor I in early embryonic loss after superovulation in the rat. Fertil Steril 1996; 65:426-436.Kuehner LF, Rieger D, Walton JS, Zhao X, Johnson WH. The effect of a depot injection of recombinant bovine somatotropin on follicular development and embryo yield in superovulated Holstein heifers. Theriogenology 1993; 40:1003-1013.Lopes AS, Butler ST, Gilbert RO, Butler WR. Relationship of pre-ovulatory follicle size, estradiol concentrations and season to pregnancy outcome in dairy cows. Anim Reprod Sci 2007; 99:34-43.Lucy MC, Byatt JC, Curran TL, Curran DF, Collier RJ. Placental lactogen and somatotropin: hormone binding to the corpus luteum and effects on the growth and functions of the ovary in heifers. Biol Reprod. 1994; 50:1136-1144.Machatkova M, Krausova K, Jokesova E, Tomanek M. Developmental competence of bovine oocytes: effects of follicle size and the phase of follicular wave on in vitro embryo production. Theriogenology 2004; 61:329-335.Márquez-Hernández CI. Somatotropina bovina recombinante y viabilidad de embriones bovinos divididos. Unpublished MSc Thesis, Universidad Autónoma Chapingo; 2011.Moraes-Júnior FJ, Torres-Júnior JR, Nascimento IM, Sousa-Junior A, Correia HS, Silva AB, Vieira RJ, Souza JAT. Efeito da somatotropina recombinante bovina (rbST) na resposta ovulatória e na qualidade dos embriões de vacas Nelore. Rev Cient Prod Anim 2008; 10:162-173.Neves EF, Ramos AF, Marques-Júnior AP. Pré-tratamento com somatotropina bovina (rbST) na superovulação de doadoras da raça Holandesa. Arq Bras Med Vet Zootec 2005; 57:205-209.Peippo J, Vartia K, Kananen-Anttila K, Räty M, Korhonen K, Hurme T, Myllymäki H, Sairanen A, Mäki-Tanila A. Embryo production from superovulated Holstein-Friesian dairy heifers and cows after insemination with frozen-thawed sex-sorted X spermatozoa or unsorted semen. Anim Reprod Sci 2009; 111:80-92.Perry GA, Smith MF, Lucy MC, Green JA, Parks TE, MacNeil MD, Roberts AJ, Geary TW. Relationship between follicle size at insemination and pregnancy success. Proc Natl Acad Sci USA 2005; 102:5268-5273.Perry GA, Smith MF, Roberts AJ, MacNeil MD, Geary TW. Relationship between size of the ovulatory follicle and pregnancy success in beef heifers. J Anim Sci 2007; 85:684-689.Rivera F, Narciso C, Olivera R, Cerri RL, Correa-Calderon A, Chebel RC, Santos JE. Effect of bovine somatotropin (500 mg) administered at ten-day intervals on ovulatory responses, expression of estrus, and fertility in dairy cows. J Dairy Sci 2010; 93:1500-1510.Roy KK, Hegde P, Banerjee K, Malhotra N, Nayyar B, Deka D, Kumar S. Fimbrio-ovarian relationship in unexplained infertility. Gynecol Obstet Invest 2005; 60:128-132.Sales JNS, Dias LMK, Viveiros ATM, Pereira MN, Souza JC. Embryo production and quality of Holstein heifers and cows supplemented with β-carotene and tocopherol. Anim Reprod Sci 2008; 106:77-89.Sartori R, Souza AH, Guenther JN, Caraviello DZ, Geiger LN, Schenk JL, Wiltbank MC. Fertilization rate and embryo quality in superovulated Holstein heifers artificially inseminated with X-sorted or unsorted sperm. Anim Reprod 2004; 1:86-90.SAS. SAS user’s guide: Statistics (Version 9.1 Ed.). SAS Inst Inc Cary NC USA 2004.Thomas FH, Campbell BK, Armstrong DG, Telfer EE. Effects of IGF-I bioavailability on bovine preantral follicular development in vitro. Reproduction 2007; 133:1121-1128.Wright JM. Photographic illustrations of embryo developmental stage and quality codes. In: Manual of the International Embryo Transfer Society. A procedural guide and general information for the use of embryo transfer technology emphasizing sanitary procedures. 4th Edition. IETS Publish Savoy USA; 2009.p. 141-144

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Published

2015-10-02

How to Cite

González Maldonado J., Rangel Santos, R., & Rodríguez de Lara R. (2015). Superovulatory response and embryo quality of Holstein heifers treated with one or two injections of somatotropin. Revista Colombiana De Ciencias Pecuarias, 28(4), 339–346. Retrieved from https://revistas.udea.edu.co/index.php/rccp/article/view/324940

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