Superovulatory response and embryo quality of Holstein heifers treated with one or two injections of somatotropin

Juan González Maldonado; Raymundo Rangel Santos; Raymundo Rodríguez de Lara

doi:10.17533/udea.rccp.324940

Autores/as

Juan González Maldonado Universidad Autónoma Chapingo
Raymundo Rangel Santos Universidad Autónoma Chapingo
Raymundo Rodríguez de Lara Universidad Autónoma Chapingo

DOI:

https://doi.org/10.17533/udea.rccp.324940

Palabras clave:

folículo preovulatorio, incidencia de celo, novillas donadoras, tasa de concepción

Resumen

Antecedentes: la somatotropina bovina recombinante (rbST) ha sido aplicada en vacas donadoras de embriones con el objetivo de mejorar la respuesta superovulatoria y la calidad embrionaria. Objetivo: este trabajo de investigación evaluó el efecto de aplicar una versus dos inyecciones de 500 mg de rbST a novillas Holstein donadoras de embriones sobre la incidencia de celo (IE), diámetro del folículo preovulatorio de mayor tamaño (dLFP), respuesta superovulatoria (SR), producción de embriones y tasa de concepción en vacas Holstein receptoras de embriones (PRR). Métodos: se llevaron a cabo dos programas de superestimulación. En cada programa las novillas fueron asignadas a uno de dos tratamientos: 1) rbST-I, n = 5: las novillas recibieron una inyección de rbST el día cero (día de remoción del CIDR (dispositivo intravaginal liberador de progesterona); 2) rbST-II, n = 5: las novillas recibieron la primera inyección de rbST el día -8 y la segunda el día cero. Treinta y ocho vacas fueron utilizadas como receptoras. Cada receptora recibió un embrión proveniente de una donadora de uno de los dos tratamientos. Resultados: la aplicación de una o dos inyecciones de rbST no afectó (p > 0,05) la PRR, dLFP, IE ni la SR. El número de ovocitos fue mayor (p < 0,05) en el tratamiento rbST-I (1,3 ± 0,4 vs 0,2 ± 0,2), el número de embriones degenerados (1,0 ± 0,5 vs 4,5 ± 3,0) o transferibles (1.0 ± 0,5 vs 1,4 ± 0,7) no fue diferente (p > 0,05) entre las vaquillas del tratamiento rbST-I y rbST-II. Además, del 36,8% de donadoras de ambos tratamientos no se colectó ningún ovocito o embrión. Conclusión: la aplicación de 500 mg de rbST los días -8 y cero del protocolo de sincronización de la onda folicular no incrementó la respuesta superovulatoria pero redujo significativamente el número de ovocitos recolectados de vaquillas Holstein superovuladas.

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