Immune and haematological parameters of Blackbelly ewes infected with gastrointestinal nematodes

Authors

  • Roberto González Garduño Chapingo Autonomous University
  • María E. López Arellano National Center for Disciplinary Research in Veterinary Parasitology
  • Magnolia M. Conde Felipe University of Las Palmas de Gran Canaria
  • Pedro Mendoza de Gives National Center for Disciplinary Research in Veterinary Parasitology
  • Liliana Aguilar Marcelino National Center for Disciplinary Research in Veterinary Parasitology
  • Gabriela Jaso Díaz Colegio de Postgraduados

DOI:

https://doi.org/10.17533/udea.rccp.v30n3a05

Keywords:

antigens, Haemonchus contortus, immunity, parasites, sheep, Trichostrongylus colubriformis

Abstract


Background: It is necessary to identify phenotypic traits related to natural resistance against gastrointestinal nematodes (GIN) in order to know the host immunity status in productive ewes. Objetive: To determine haematological and immunological parameters (IgA and IgG) during pregnancy and lactation in Blackbelly ewes naturally infected with GIN. Methods: The number of eggs per gram (EPG), packed cell volume (% PCV), plasmatic protein (PP), and peripheral eosinophils were determined during eight months. In addition, sera and saliva samples were collected to establish IgG and IgA kinetics by indirect enzyme-linked immunosorbent assay (ELISA). Results: The results showed 2,592 ± 2,403 EPG and 22.2 ± 4.0% PCV during lactation and 595 ± 901 EPG and 25.1 ± 2.5% PCV during pregnancy. A higher percentage of Trichostrongylus colubriformis larvae were observed in pregnancy (84 to100%) than in lactation (36 to 44%). The IgA activity in serum samples showed a marked reduction (from 80 to 10%) during lambing for both Haemonchus contortus and T. colubriformis antigens. In saliva samples, IgA activity with regard to the standard decreased from 56% at 60 days to 30% at 45 days before lambing and remained low for 45 days during lactation (23 to 32% activity). The eosinophils numbers were 2.0 x 109 cells L-1 in pregnancy and remained low at 0.7 x 109 cells L-1 in lactation. Conclusion: The studied variables reflect the breakdown of immunity against GIN in Blackbelly ewes before and after lambing.

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Author Biographies

Roberto González Garduño, Chapingo Autonomous University

AEZ., Dr., South-Southeast Regional University Unit, Chapingo Autonomous University, Teapa, Tabasco, Mexico.

María E. López Arellano, National Center for Disciplinary Research in Veterinary Parasitology

VMZ, PhD., National Center for Disciplinary Research in Veterinary Parasitology (INIFAP), Jiutepec, Morelos, Mexico.

Magnolia M. Conde Felipe, University of Las Palmas de Gran Canaria

VM, PhD., Faculty of Veterinary Medicine, University of Las Palmas de Gran Canaria, Las Palmas, Spain.

Pedro Mendoza de Gives, National Center for Disciplinary Research in Veterinary Parasitology

VMZ, PhD., National Center for Disciplinary Research in Veterinary Parasitology (INIFAP), Jiutepec, Morelos, Mexico.

Liliana Aguilar Marcelino, National Center for Disciplinary Research in Veterinary Parasitology

AEZ, Dr., National Center for Disciplinary Research in Veterinary Parasitology (INIFAP), Jiutepec, Morelos, Mexico.

Gabriela Jaso Díaz, Colegio de Postgraduados

VMZ, MC., Colegio de Postgraduados, Montecillo Campus, Montecillo, State of Mexico, Mexico.

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Published

2017-07-24

How to Cite

González Garduño, R., López Arellano, M. E., Conde Felipe, M. M., Mendoza de Gives, P., Aguilar Marcelino, L., & Jaso Díaz, G. (2017). Immune and haematological parameters of Blackbelly ewes infected with gastrointestinal nematodes. Revista Colombiana De Ciencias Pecuarias, 30(3), 219–230. https://doi.org/10.17533/udea.rccp.v30n3a05

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