Fishmeal substitution for Arthrospira platensis in juvenile mullet (Mugil liza) and its effects on growth and non-specific immune parameters

Victor Torres Rosas; Martin Bessonart; Luis Alberto Romano; Marcelo Borges Tesser

doi:10.17533/udea.rccp.v32n1a01

Authors

Victor Torres Rosas Federal University of Rio Grande
Martin Bessonart University of the Republic, National Directorate for Aquatic Resources
Luis Alberto Romano Federal University of Rio Grande
Marcelo Borges Tesser Federal University of Rio Grande

DOI:

https://doi.org/10.17533/udea.rccp.v32n1a01

Keywords:

apoptosis, CD3, fish, immunostimulant, microalgae, Spirulina

Abstract

Background: Cyanobacterium Athrospira platensis (Spirulina) is a potential fishmeal (FM) substitute in fish diets because of its high protein content, antioxidant and immunostimulant properties. Objective: To evaluate the effects of total and partial substitution of FM with A. platensis (0, 30, 50, 70 and 100% substitution) in juvenile mullet (Mugil liza). Methods: Juvenile mullets (n=210) were maintained in a recirculation system under optimal water parameters for the species. Mullets were fed five experimental diets for 80 days. Each diet was tested in triplicate tanks. At the end of the experimental period growth parameters were measured and samples of blood, liver and spleen were taken to evaluate the immune system. Results: Full replacement (100%) of FM resulted in growth deficits and low survival. The FM replacement induced changes in the proportion of macrophages and lymphocytes. Up to 50% FM replacement increased the expression of CD3 receptors in spleen T lymphocytes (T-Cells), whereas >50% FM replacement decreased the expression of CD3 receptors. We also found that partial FM substitution diminished the apoptotic process. Conclusions: Up to 50% FM substitution with A. platensis can improve performance of non-specific immune system of mullets.

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Author Biographies

Victor Torres Rosas, Federal University of Rio Grande

VMZ, MSc., Aquatic Organisms Nutrition Laboratory, Institute of Oceanography (IO), Federal University of Rio Grande (FURG), Rio Grande, Brazil.

Martin Bessonart, University of the Republic, National Directorate for Aquatic Resources

PhD., Natural Resources Laboratory, Faculty of Sciences, University of the Republic (UDeLaR), Montevideo, Uruguay. Marine Research and Aquaculture Experimental Station, National Directorate for Aquatic Resources (DINARA), Cabo Polonio, Uruguay.

Luis Alberto Romano, Federal University of Rio Grande

PhD., Laboratory of Pathology and Immunology of Aquatic Organisms, Institute of Oceanography (IO), Federal University of Rio Grande (FURG), Rio Grande, Brazil.

Marcelo Borges Tesser, Federal University of Rio Grande

PhD., PhD., Laboratory of Nutrition of Aquatic Organisms, Institute of Oceanography (IO), Federal University of Rio Grande (FURG), Rio Grande, Brazil.

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