The spawning method affects the reproductive efficiency of piracanjuba fish (Brycon orbignyanus) in restocking programs

  • Pedro L. de Castro State University of Mato Grosso do Sul
  • Nelson M. Lopera-Barrero State University of Londrina
  • Elenice S. dos Reis-Goes Federal University of Grande Dourados
  • Felipe P. de Souza State University of Londrina
  • Satia C. Bomfim State University of Mato Grosso do Sul
  • André L. Julien-Ferraz State University of Mato Grosso do Sul
  • Ricardo P. Ribeiro State University of Maringá
Keywords: aquaculture, breeders, broodfish, broodstock, Brycon orbignyanus, fertilization, fish, genetic diversity, hatching, microsatellite markers, mortality, reproductive efficiency, restocking, spawning, strip-spawning

Abstract

Background: Preserving the genetic diversity of wild fish is an important consideration for restocking programs, as inbreeding can compromise progeny survival as well as impact the resilience of natural populations. Objective: To evaluate the influence of spawning method: semi-natural (SN) or strip-spawning (ST), and the number of breeders (1♀:3♂ and 2♀:6♂) on the reproductive efficiency and genetic diversity of B. orbignyanus progeny destined for restoration of wild stocks. Methods: Rates of fertilization, hatching and broodfish mortality were recorded. For genetic evaluations (allele frequency, observed and expected heterozygosity, Shannon index, inbreeding coefficient, molecular variance analysis, and genetic differentiation), breeders (n=24), and their progenies (90 larvae/treatment) were sampled and analyzed using eight microsatellite markers. Results: Higher fertilization and hatching rates, and lower broodfish mortality were observed for the SN method (p<0.05), whereas the number of breeders did not affect these parameters (p>0.05). Interaction between spawning method and number of breeders was not significant (p>0.05). The amplified microsatellite loci produced a total of 30 alleles, with sizes between 80 and 225 bp and their frequencies indicated an increase (p<0.05) of genetic diversity in the progenies, but low genetic differentiation between treatments (p>0.05). Conclusion: The spawning methods and number of breeders tested increased equally the genetic diversity of the progeny, with low genetic differentiation between treatments. In contrast, rates of fertilization, hatching and brood fish mortality revealed that the SN method resulted in the best reproductive efficiency due to the handling stress and injuries caused by ST. Thus, SN proves to be the most suitable spawning-method for B. orbignyanus in restocking programs.

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Author Biographies

Pedro L. de Castro, State University of Mato Grosso do Sul

https://orcid.org/0000-0001-7351-6938
Animal Science Postgraduate Program, State University of Mato Grosso do Sul, Aquidauana, Mato Grosso do Sul, Brazil.

Nelson M. Lopera-Barrero, State University of Londrina

https://orcid.org/0000-0002-7154-5134
Animal Science Postgraduate Program, State University of Londrina, Londrina, Paraná, Brazil.

Elenice S. dos Reis-Goes, Federal University of Grande Dourados

https://orcid.org/0000-0003-2437-4800
Fishery engineering department, Federal University of Grande Dourados, Dourados, Mato Grosso do Sul, Brazil.

Felipe P. de Souza, State University of Londrina

https://orcid.org/0000-0003-3436-4147
Animal Science Postgraduate Program, State University of Londrina, Londrina, Paraná, Brazil.

Satia C. Bomfim, State University of Mato Grosso do Sul

https://orcid.org/0000-0002-6615-7700
Animal Science Postgraduate Program, State University of Mato Grosso do Sul, Aquidauana, Mato Grosso do Sul, Brazil.

André L. Julien-Ferraz, State University of Mato Grosso do Sul

https://orcid.org/0000-0002-0538-2867
Animal Science Postgraduate Program, State University of Mato Grosso do Sul, Aquidauana, Mato Grosso do Sul, Brazil.

Ricardo P. Ribeiro, State University of Maringá

https://orcid.org/0000-0001-7752-3692
Animal Science department, State University of Maringá, Maringá, Paraná, Brazil.

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Published
2020-06-17
Section
Original research articles