Presence of Chlamydia abortus in colostrum, milk and vaginal discharge samples of sheep

Authors

  • María Guadalupe Martínez-Serrano Universidad Nacional Autónoma de México
  • Efrén Díaz-Aparicio Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias
  • Gabriela Palomares-Reséndiz Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias
  • Jorge Luis Tórtora-Pérez Universidad Nacional Autónoma de México
  • Hugo Ramírez-Álvarez Universidad Nacional Autónoma de México
  • Nieves Ortega-Hernández Universidad de Murcia
  • Jesús Salinas-Lorente Universidad de Murcia
  • José Francisco Morales-Alvarez Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias
  • Juan Julio César Cervantes-Morali Universidad Nacional Autónoma de México

DOI:

https://doi.org/10.17533/udea.rccp.v35n2a04

Keywords:

Chlamydia abortus, chlamydial infection, colostrum, ewes, milk, ovine, secretion, sheep, vaginal discharge

Abstract

Background: The main transmission route of Chlamydia abortus is by ingesting the microorganism that has been eliminated in vaginal secretions, placental membranes or abortions that contaminate the environment and, possibly, through milk and colostrum. Elimination through vaginal secretions is well documented. However, there are no reports about isolation and identification of C. abortus in the colostrum or milk of infected sheep, so it is important to determine whether or not C. abortus may be present in these secretions, which are the only food of lambs. Objective: To detect C. abortus in colostrum, milk, and vaginal secretions of sheep with a history of reproductive disorders. Methods: Colostrum, milk, and vaginal exudates were collected from 66 sheep. The samples were inoculated in mouse fibroblast cell cultures and the presence of C. abortus determined by direct immunofluorescence. Results: 19 out of 66 colostrum samples (28.7%), 14 out of 66 milk samples (21.2%) and 17 out of 66 vaginal swabs (25.7%) were positive for C. abortus. The 50 samples positive for isolation and detected by immunofluorescence, together with 42 negative samples were subjected to qPCR to amplify a fragment of the ompA gene from C. abortus. Thirty-eight of the 92 samples processed by this technique were positive for C. abortus. Conclusion: The results demonstrated the presence of C. abortus in a high proportion in colostrum, milk and vaginal secretions of infected sheep. To the best of our knowledge, this is the first field study confirming the presence of C. abortus in colostrum, which shows that excretion of Chlamydia by lactogenesis could occur in the first hours after birth.

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Author Biographies

María Guadalupe Martínez-Serrano, Universidad Nacional Autónoma de México

Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510, Coyoacán, Ciudad de México.
https://orcid.org/0000-0002-7096-2227

Efrén Díaz-Aparicio, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias

Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias. Carretera Federal México Toluca, km 15.5, Colonia Palo Alto, Cuajimalpa, 05110 Ciudad de México
https://orcid.org/0000-0002-1669-1323

Gabriela Palomares-Reséndiz, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias

Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias. Carretera Federal México Toluca, km 15.5, Colonia Palo Alto, Cuajimalpa, 05110 Ciudad de México.
https://orcid.org/0000-0001-5561-338X

Jorge Luis Tórtora-Pérez, Universidad Nacional Autónoma de México

Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, San Sebastián Xhala, 54714 Cuautitlán Izcalli, Estado de México.
https://orcid.org/0000-0002-8085-5193

Hugo Ramírez-Álvarez, Universidad Nacional Autónoma de México

Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, San Sebastián Xhala, 54714 Cuautitlán Izcalli, Estado de México.
https://orcid.org/0000-0003-1682-8104

Nieves Ortega-Hernández, Universidad de Murcia

Facultad de Veterinaria, Universidad de Murcia, España. Calle Campus Universitario, 7, 30100 Murcia, España.
https://orcid.org/0000-0002-0941-8441

Jesús Salinas-Lorente, Universidad de Murcia

Facultad de Veterinaria, Universidad de Murcia, España. Calle Campus Universitario, 7, 30100 Murcia, España.
https://orcid.org/0000-0001-8573-4780

José Francisco Morales-Alvarez, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias

Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias. Carretera Federal México Toluca, km 15.5, Colonia Palo Alto, Cuajimalpa, 05110 Ciudad de México.
Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, San Sebastián Xhala, 54714 Cuautitlán Izcalli, Estado de México
https://orcid.org/0000-0001-5432-6653

Juan Julio César Cervantes-Morali, Universidad Nacional Autónoma de México

Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510, Coyoacán, Ciudad de México.
https://orcid.org/0000-0003-2272-9209

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Published

2022-07-11

How to Cite

Martínez-Serrano, M. G., Díaz-Aparicio, E., Palomares-Reséndiz, G., Tórtora-Pérez, J. L., Ramírez-Álvarez, H., Ortega-Hernández, N., Salinas-Lorente, J., Morales-Alvarez, J. F., & Cervantes-Morali, J. J. C. (2022). Presence of Chlamydia abortus in colostrum, milk and vaginal discharge samples of sheep. Revista Colombiana De Ciencias Pecuarias, 35(3), 165–173. https://doi.org/10.17533/udea.rccp.v35n2a04

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Original research articles