Presence of Chlamydia abortus in colostrum, milk and vaginal discharge samples of sheep

Authors

  • María Guadalupe Martínez-Serrano Universidad Nacional Autónoma de México
  • Efrén Díaz-Aparicio Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias
  • Gabriela Palomares-Reséndiz Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias
  • Jorge Luis Tórtora-Pérez Universidad Nacional Autónoma de México
  • Hugo Ramírez-Álvarez Universidad Nacional Autónoma de México
  • Nieves Ortega-Hernández Universidad de Murcia
  • Jesús Salinas-Lorente Universidad de Murcia
  • José Francisco Morales-Alvarez Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias
  • Juan Julio César Cervantes-Morali Universidad Nacional Autónoma de México

DOI:

https://doi.org/10.17533/udea.rccp.v35n2a04

Keywords:

Chlamydia abortus, chlamydial infection, colostrum, ewes, milk, ovine, secretion, sheep, vaginal discharge

Abstract

Background: The main transmission route of Chlamydia abortus is by ingesting the microorganism that has been eliminated in vaginal secretions, placental membranes or abortions that contaminate the environment and, possibly, through milk and colostrum. Elimination through vaginal secretions is well documented. However, there are no reports about isolation and identification of C. abortus in the colostrum or milk of infected sheep, so it is important to determine whether or not C. abortus may be present in these secretions, which are the only food of lambs. Objective: To detect C. abortus in colostrum, milk, and vaginal secretions of sheep with a history of reproductive disorders. Methods: Colostrum, milk, and vaginal exudates were collected from 66 sheep. The samples were inoculated in mouse fibroblast cell cultures and the presence of C. abortus determined by direct immunofluorescence. Results: 19 out of 66 colostrum samples (28.7%), 14 out of 66 milk samples (21.2%) and 17 out of 66 vaginal swabs (25.7%) were positive for C. abortus. The 50 samples positive for isolation and detected by immunofluorescence, together with 42 negative samples were subjected to qPCR to amplify a fragment of the ompA gene from C. abortus. Thirty-eight of the 92 samples processed by this technique were positive for C. abortus. Conclusion: The results demonstrated the presence of C. abortus in a high proportion in colostrum, milk and vaginal secretions of infected sheep. To the best of our knowledge, this is the first field study confirming the presence of C. abortus in colostrum, which shows that excretion of Chlamydia by lactogenesis could occur in the first hours after birth.

|Abstract
= 752 veces | PDF
= 414 veces| | HTML
= 0 veces|

Downloads

Download data is not yet available.

Author Biographies

María Guadalupe Martínez-Serrano, Universidad Nacional Autónoma de México

Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510, Coyoacán, Ciudad de México.
https://orcid.org/0000-0002-7096-2227

Efrén Díaz-Aparicio, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias

Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias. Carretera Federal México Toluca, km 15.5, Colonia Palo Alto, Cuajimalpa, 05110 Ciudad de México
https://orcid.org/0000-0002-1669-1323

Gabriela Palomares-Reséndiz, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias

Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias. Carretera Federal México Toluca, km 15.5, Colonia Palo Alto, Cuajimalpa, 05110 Ciudad de México.
https://orcid.org/0000-0001-5561-338X

Jorge Luis Tórtora-Pérez, Universidad Nacional Autónoma de México

Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, San Sebastián Xhala, 54714 Cuautitlán Izcalli, Estado de México.
https://orcid.org/0000-0002-8085-5193

Hugo Ramírez-Álvarez, Universidad Nacional Autónoma de México

Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, San Sebastián Xhala, 54714 Cuautitlán Izcalli, Estado de México.
https://orcid.org/0000-0003-1682-8104

Nieves Ortega-Hernández, Universidad de Murcia

Facultad de Veterinaria, Universidad de Murcia, España. Calle Campus Universitario, 7, 30100 Murcia, España.
https://orcid.org/0000-0002-0941-8441

Jesús Salinas-Lorente, Universidad de Murcia

Facultad de Veterinaria, Universidad de Murcia, España. Calle Campus Universitario, 7, 30100 Murcia, España.
https://orcid.org/0000-0001-8573-4780

José Francisco Morales-Alvarez, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias

Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias. Carretera Federal México Toluca, km 15.5, Colonia Palo Alto, Cuajimalpa, 05110 Ciudad de México.
Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, San Sebastián Xhala, 54714 Cuautitlán Izcalli, Estado de México
https://orcid.org/0000-0001-5432-6653

Juan Julio César Cervantes-Morali, Universidad Nacional Autónoma de México

Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510, Coyoacán, Ciudad de México.
https://orcid.org/0000-0003-2272-9209

References

Barati S, Moori-Bakhtiari N, Najafabadi MG, Momtaz H, Shokuhizadeh L. The role of zoonotic chlamydial agents in ruminants abortion. Iranian J of Microbiol 2017; 9(5):288–294. URL: https://pubmed.ncbi.nlm.nih.gov/29296274/

Castro N, Capote J, Bruckmaier RM, Argüello A. Management effects on colostrogenesis in small ruminants: A review. J Appl Anim Res 2011; 39(2):85–93. DOI: https://doi.org/10.1080/09712119.2011.581625

Elwell C, Mirrashidi K, Engel J. Chlamydia cell biology and pathogenesis. Nature Rev Microbiol 2016; 14(6):385–400. DOI: https://doi.org/10.1038/nrmicro.2016.30

Escuder VD, Espinosa MI, Rodríguez JM, Fernández L, Pallás ACR. Effect of HTST and holder pasteurization on the concentration of immunoglobulins, growth factors, and hormones in donor human milk. Front Immunol 2018. 9:2222. DOI: https://doi.org/10.3389/fimmu.2018.02222

Escuder VD, Rodríguez JM, Espinosa MI, Corzo N, Montilla A, García SA, Visitación CM, Fontecha J, Serrano J, Fernández L, Pallás ACR. High-temperature short-time and holder pasteurization of donor milk: impact on milk composition. Life 2021; 11(2):114. DOI: https://doi.org/10.3390/life11020114

Livingstone M, Wheelhouse N, Ensor H, Rocchi M, Maley S, Aitchison K, Wattegedera S, Wilson K, Sait M, Siarkou V, Vretou E, Entrican G, Dagleish M, Longbottom D. Pathogenic outcome following experimental infection of sheep with Chlamydia abortus variant strains LLG and POS. PLoS ONE 2017; 12(5):1–19. DOI: https://doi.org/10.1371/journal.pone.0177653

Longbottom D, Coulter LJ. Animal chlamydioses and zoonotic implications. J Comp Pathology 2003; 128(4):217–244. DOI: https://doi.org/10.1053/jcpa.2002.0629

Nietfeld JC. Chlamydial infections in small ruminants. The Veterinary Clinics of North America. Food Anim Practice 2001; 17(2):301–314. DOI: https://doi.org/10.1016/S0749-0720(15)30030-X

Ortega N, Caro M, Gallego C, Murcia-Belmonte A, Alvarez D, del Rio L, Cuello F, Buendía A, Salinas J. Isolation of Chlamydia abortus from a laboratory worker diagnosed with atypical pneumonia. Irish Vet J 2016; 69(8):1-4. DOI: https://doi.org/10.1186/s13620-016-0067-4

Oseikria M, Pellerin J, Rodolakis A, Vorimore F, Laroucau K, Bruyas J, Roux C, Michaud S, Larrat M, Fieni F. Can Chlamydia abortus be transmitted by embryo transfer in goats? Theriogenology 2016 (86): 1482–1488. DOI: http://dx.doi.org/10.1016/j.theriogenology.2016.05.006

Pantchev A, Sting R, Bauerfeind R, Tyczka J, Sachse K. New real-time PCR tests for species-specific detection of Chlamydophila psittaci and Chlamydophila abortus from tissue samples. Vet J 2009; 181(2):145–150. DOI: https://doi.org/10.1016/j.tvjl.2008.02.025

Papp JR, Shewen PE, Gartley CJ. Abortion and subsequent excretion of chlamydiae from the reproductive tract of sheep during estrus. Infect and Immun 1994; 62(9):3786–3792. DOI: https://doi.org/10.1128/iai.62.9.3786-3792.1994

Rocchi MS, Wattegedera S, Meridiani I, Entrican G. Protective adaptive immunity to Chlamydophila abortus infection and control of ovine enzootic abortion (OEA). Vet Microbiol 2009; 135(1–2):112–121. DOI: https://doi.org/10.1016/j.vetmic.2008.09.030

Rodolakis A, Laroucau K. Chlamydiaceae and chlamydial infections in sheep or goats. Vet Microbiol 2015; 181(1–2):107–118. DOI: https://doi.org/10.1016/j.vetmic.2015.07.010

Rojas M, Fort M, Bettermann S, Entrocassi C, Costamagna S, Sachse K, Rodríguez M. Detección de Chlamydia abortus en pérdidas reproductivas de bovinos en la provincial de La Pampa, Argentina. Rev Argent Microbiol 2018; 50 (3):269-274. DOI: https://doi.org/10.1016/j.ram.2017.10.002

Salinas J, Sanchez J, Buendia AJ, Souriau A, Rodolakis A, Bernabé A, Cuello F. The LPS localization might explain the lack of protection of LPS-specific antibodies in abortion-causing Chlamydia psittaci infections. Res Microbiol 1994; 145(8). DOI: https://doi.org/10.1016/0923-2508(94)90078-7

Sambrook J, Fritsch EF, MT. Molecular cloning a laboratory manual, Volumes 1, 2 and 3. 2nd ed. Cold Spring Harbor Laboratory Press; 1989; ISBN:0-87969-309-6. URL: https://is.muni.cz/publication/372112/en/Molecular-Cloning-A-laboratory-Manual/Sambrook-Fritsch-Maniatis

Selim A, Manaa E, Waheed R, Alanazi A. Seroprevalence, associated risk factors analysis and first molecular characterization of Chlamydia abortus among Egyptian sheep 2020; 74. DOI: https://doi.org/10.1016/j.cimid.2020.101600

Stuen S, Longbottom D. Treatment and control of chlamydial and rickettsial infections in sheep and goats. Vet Clin N Am-Food Anim Pract 2011; 27(1): 213–233. DOI: https://doi.org/10.1016/j.cvfa.2010.10.017

Thomas R, Davison HC, Wilsmore AJ. Use of the IDEIA ELISA to detect Chlamydia psittaci (ovis) in material from aborted fetal membranes and milk from ewes affected by ovine enzootic abortion. British Vet J 1990; 146(4):364–367. DOI: https://doi.org/10.1016/S0007-1935(11)80031-X

Wattegedra SR, Livingstone M, Maley S, Rocchi M, Lee S, Pang Y, Wheelhouse NM, Aitchison K, Palarea AJ, Buxton D, Longbottom D, Entrican G. Defining immune correlates during latent and active chlamydial infection in sheep. Vet Res 2020; 51(2):75. DOI: https://doi.org/10.1186/s13567-020-00798-6.

Downloads

Published

2022-07-11

How to Cite

Martínez-Serrano, M. G., Díaz-Aparicio, E., Palomares-Reséndiz, G., Tórtora-Pérez, J. L., Ramírez-Álvarez, H., Ortega-Hernández, N., Salinas-Lorente, J., Morales-Alvarez, J. F., & Cervantes-Morali, J. J. C. (2022). Presence of Chlamydia abortus in colostrum, milk and vaginal discharge samples of sheep. Revista Colombiana De Ciencias Pecuarias, 35(3), 165–173. https://doi.org/10.17533/udea.rccp.v35n2a04

Issue

Section

Original research articles