Characterization and antimicrobial resistance of Moraxella ovis isolates from clinical cases of contagious ovine keratoconjunctivitis in Mexico State, Mexico
DOI:
https://doi.org/10.17533/udea.rccp.v37n1a4Keywords:
antimicrobial resistance, contagious ovine keratoconjunctivitis, epidemiological surveillance, goat, Moraxella spp, multiresistance, resistance genes, sheepAbstract
Background: Contagious ovine keratoconjunctivitis (OKC) causes blindness in sheep and goats and it is associated with a set of bacterial genera of which some species show antimicrobial resistance. Objective: To identify phenotypic-genotypic relationship of antimicrobial resistance from Moraxella spp. isolates obtained from clinical cases of contagious ovine keratoconjunctivitis (OKC) in Mexico. Methods: A total of 209 samples were obtained from clinical cases of OKC in sheep and 60 Moraxella ovis isolates were identified by bacteriological techniques and amplification of 16s rRNA and rtxA genes by PCR. All isolates were evaluated in terms of antimicrobial resistance by the disk diffusion susceptibility test and amplification of resistance genes by PCR. Results: We found 14 Moraxella ovis isolates with antimicrobial resistance (AMR) and five multiresistant (MDR). The sul1, sul2, tetB, qnrA, qnrB, BlaTEM genes of antimicrobial resistance were amplified, while gene floR was not amplified. Conclusion: This is the first isolation report of Moraxella ovis from ocular lesions in sheep in the State of Mexico, with six antimicrobial resistance genes identified. Results suggest that Moraxella ovis plays an important role in the course of the disease and provides a panorama for molecular epidemiological surveillance and bacterial resistance.
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