Identifying signatures of recent selection in Holstein cattle in the tropic

Juan C. Rincón; Albeiro López; Julián Echeverri

doi:10.17533/udea.rccp.v31n1a06

Autores/as

Juan C. Rincón Universidad Tecnológica de Pereira, Universidad Nacional de Colombia
Albeiro López Universidad Nacional de Colombia
Julián Echeverri Universidad Nacional de Colombia

DOI:

https://doi.org/10.17533/udea.rccp.v31n1a06

Palabras clave:

ganado lechero, mapeo genético, QTLs, presión de selección, polimorfismos de nucleótido simple

Resumen

Antecedentes: El ganado Holstein ha sido sometido a procesos fuertes de selección en el mundo. Estas señales de selección pueden ser reconocidas y utilizadas para identificar regiones del genoma importantes para la producción de leche. Objetivo: Identificar señales de selección recientes en ganado Holstein de la Provincia de Antioquia (Colombia), mediante la metodología de puntaje haplotípico integrado (iHS). Métodos: A 150 animales se les extrajo DNA de sangre o semen mediante un kit comercial y posteriormente se genotiparon los animales con el chip BovineLD (6909 SNPs). Se realizó edición conservando los loci con frecuencia del alelo menor (MAF) superior a 0,05. Además, se descartaron los genotipos con errores mendelianos, usando el software R y PLINK v1.07. La determinación de la homocigosidad haplotípica extendida (EHH), iHS y el valor p se realizó utilizando el paquete “rehh” de R. Resultados: Las frecuencias del alelo menor mostraron una tendencia hacia alelos de frecuencias intermedias. En total, 144 marcadores focales fueron significativos (p < 0,001) para las señales de selección. Algunos cromosomas presentaron mayor número de señales de selección que otros. Muchas de las variantes focales se encontraron al interior de genes, aunque comúnmente en regiones intrónicas. Algunas de las regiones importantes estuvieron asociadas con genes como TRAPPC12, PANK3, ZNF16, OPLA y DPYSL4 que en general se encuentran asociados con funciones relacionadas con el transporte, excreción o metabolismo celular. Conclusión: La identificación de señales de selección usando el método iHS permitió determinar algunas regiones importantes para la selección en ganado Holstein del trópico alto, algunas de las cuales han sido previamente reportadas por su asociación a loci de características cuantitativas (QTLs).

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Biografía del autor/a

Juan C. Rincón, Universidad Tecnológica de Pereira, Universidad Nacional de Colombia

Zoot, MSc, PhD., Programa de Medicina Veterinaria y Zootecnia, Universidad Tecnológica de Pereira, Risaralda, Pereira, Colombia. Grupo de Investigación Biodiversidad y Genética Molecular (BIOGEM), Facultad de Ciencias Agrarias, Departamento de Producción Animal, Universidad Nacional de Colombia, sede Medellín, Colombia.

Albeiro López, Universidad Nacional de Colombia

MV, Zoot, MSc, PhD., Grupo de Investigación Biodiversidad y Genética Molecular (BIOGEM), Facultad de Ciencias Agrarias, Departamento de Producción Animal, Universidad Nacional de Colombia, sede Medellín, Colombia.

Julián Echeverri, Universidad Nacional de Colombia

Zoot, MSc, PhD., Grupo de Investigación Biodiversidad y Genética Molecular (BIOGEM), Facultad de Ciencias Agrarias, Departamento de Producción Animal, Universidad Nacional de Colombia, sede Medellín, Colombia.

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