Estandarización del aislamiento y caracterización de células estromales de decidua y útero murinos

Lina Cadavid; Cristina Ávila; Angela Patricia Cadavid

doi:10.17533/udea.rccp.324497

Autores

Lina Cadavid
Cristina Ávila
Angela Patricia Cadavid

DOI:

https://doi.org/10.17533/udea.rccp.324497

Palavras-chave:

BeWo cells, decidual macrophages, HeLa cells, murine decidua, stromal cells

Resumo

As células estromales são as células mais abundantes presentes na decídua e tem um papel muito importante durante a implementação, a nutrição fetal e a manutenção da gravidez. Os procedimentos descritos para o isolamento das células estromales necessitam o uso de muitos anticorpos monoclonais já que há contaminação com outros tipos celulares na decídua e demais alguns marcadores característicos das células estromales que tem variabilidade nos diferentes dias de gestação. No presente trabalho foi estandardizado um procedimento de isolamento por digestão enzimática, gradiente de densidade e aderência ao plástico e foram caracterizados as células estromales murinas por exclusão de marcadores que expressão em macrófagos (F4-80), células epiteliais e trofoblasto (citoqueratina-7), obtendo-se um 98% de células negativas para estes marcadores que corresponderia às células estromales. Esta técnica de isolamento permite obter células estromales com métodos menos custosos e altamente eficientes que facilita o aceso a um modelo celular de grande utilidade no estudo da fisiologia da gestação em diferentes espécies.

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