Determinación de la concentración de esmolol en plasma por cromatografía gaseosa acoplada a un detector nitrogeno-fósforo
DOI:
https://doi.org/10.17533/udea.vitae.453Keywords:
esmolol, β-bloqueadores, niveles plasmàticos, cromatografia de gases, validaciòn, β-blockers, plasmatic levels, gas chomatography, validationAbstract
Este artículo describe el desarrollo y validación de un método analítico alternativo para la cuantificación de esmolol, un β-bloqueador de acción ultra corta, en plasma humano, usando un cromatógrafo de gases acoplado a un detector de Nitrógeno–Fósforo. El procedimiento analítico es preciso y exacto. Con una precisión en el rango de: 4.1% al 19.7%, una exactitud en un rango de: 2.5% al 25.9%. La curva es lineal para concentraciones de concentraciones en el rango de 6,25-1000 ng/ml; con un coeficiente de correlación promedio de 0.9995 cuando se usa como estándar interno propranolol. Los tiempos de retención para el esmolol y el propanolol son de 12.87 ± 0.04 min y 11.65 ± 0.05 min respectivamente. El procedimiento es simple y sensible ya que no requiere de reextracción o derivatización del esmolol y su límite de cuantificación es de 6.25 ng/mL. Por esto, permite medir concentraciones plasmáticas de esmolol en individuos a los que se les administren dosis bajas.Downloads
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