Transient expression of the β glucuronidasa gene on somatic embryos of Heliconia stricta by Agrobacterium tumefaciens mediated transformation
DOI:
https://doi.org/10.17533/udea.acbi.329509Keywords:
H. stricta, genetic transformation, A. tumefaciens, transient expression, β glucuronidasaAbstract
Factors such as the bacterial strain preculture time and coculture time with the bacteria were evaluated in terms of the transient expression of gene Gus on somatic embryos of Heliconia stricta. The results showed that the bacteria strain and coculture time had significant effects on the percentage of embryos with transient expression, but not the preculture time.The strain LBA4404 with the plasmid pBI121, with three days of coculture, showed the best transient expression of the gene Gus in this specie. However, it will be necessary to evaluate the effect of certain antibiotics and longer coculture times on the process after transformation, in order to optimize a protocol that allows for the stable transformation of the embryos.
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Aldemita L, Hodges T. 1996. Agrobacteriumtumefaciens-mediatedtransformation of Japonica and Indica rice varieties. Planta199:612-617.Belarmino M, Mii M. 2000. Agrobacterium mediated genetictransformation of a Phalaenopsis orquid. Plant Cell Reports19:435-442.Berry F, Kress J. 1991. Heliconia: an identification guide.Smithsonian Institution Press. Hong Kong, China. 334 p.Bi Y, Cammue B, Krisnah Raj S, Saxena P. 1999. Resistance toBotrytis cinerea in scented Geranium transformed with agene encoding the antimicrobial protein Ace- AMP1. PlantCell Reports 18:835-840.Bytebier B, Deboeck F, Debreve H, Van Montagu M,Hernalsteens JP. 1987. T-DNA Organization in TumorCultures and Transgenic Plants of the MonocotyledonAsparagus officinalis. Proc Natl Acad Sci USA 84:5.345-5.349.Cao X, Liu Q, Rowland LJ, Hammerschlag FA. 1998. Gusexpression in blueberry factors influencing Agrobacterium-mediated gene transfer efficiency. Plant Cell Reports 18:266-270.Cervera M, Pina JA, Juárez J, Navarro L, Peña L. 1998.Agrobacterium-mediated transformation of citrange: factorsaffecting transformation and regeneration. Plant CellReports 18(3-4):271-278.Curtis L, Power J, Heoden P, Phillips A. 1999. A stabletransformation system for de ornamental plant, Daturameteloides D C. Plant Cell Reports 18:554-560.Chauvin J, Marhadour S, Cohat J, Le Nard M. 1999. Effects ofgelling agents on in vitro regeneration and kanamycinefficiency as selective agent in plant transformationprocedures. Plant Cell Tissue Organ Culture 58:213-217.De Bondt A, Eggermont,K, Druart P, De Vil M. 1994.Agrobacterium-mediated transformation of apple: anassesment of factors affecting gene transfer efficiency duringearly transformation steps. Plant Cell Reports 13:587-593.Deroles S, Boase M, Konczak L. 1997. Transformation protocolsfor ornamental plants. In: Geneve RL, Preece J E, Merkle S(eds.). Biotechnology of ornamental plants. CABINTERNATIONAL. Canadá, pp. 87-118
Dong J, Teng W, Bucholz W, Hall T. 1996. Agrobacteriummediated transformation of Javanica rice. Mol Breeding2:267-276.Eady C, Lister C. 1998. A comparison of four selective agentsfor use with Allium cepa L. immature embryos and immatureembryos derived cultures. Plant Cell Reports 18:117-121.Gasser CS, Fraley R. 1989. Genetically engineering plants forCrop Improvement. Science 244:1.293-1.299.Giovannini A, Zottini M, Morreale G, Spena A, Allavena A.1999. Ornamental traits modification by rol genes inOsteospermum ecklonis transformed with Agrobacteriumtumefaciens. In Vitro Cell Dev Biol Plant 35:70-75.Glick BR, Thompson JE. 1993. Methods in plant molecularbiology and biotechnology. CRC Press, Inc. United States.Gutterson NC, Napoli C, Morgan A. 1994. Modification offlower color in florist Chrysanthemum: production of awhite flowering variety through molecular genetics.Biotechnology 12:268-271.Haymes K, Davis T. 1998. Agrobacterium mediated transformationof ‘Alpine’ Fragaria vesca and transmission of trangenes toR1 progeny. Plant Cell Reports 17:279-283.Hiei Y, Ohta S, Komari T, Kumashiro T. 1994. Efficienttransformation fo rice (Oryza sativa L.) mediated byAgrobacterium and sequence analysis of the boundaries ofT-DNA. Plant Journal 6(2):271-282.Hiei Y, Komari T, Kubo T. 1997. Transformation of rice mediatedby Agrobacterium tumefaciens. Plant Mol Biol 35:205-218.Hoekema A, Hirsch PR, Hooykaas PJ, Schilperoort RA. 1983.A binary plant vector strategy based on separation of virand Ti region of the Agrobacterium tumefaciens Ti-plasmid.Nature 303:179-180.Hutchinson JF, Kaul V, Maheswaran G, Moran JR, GrahamM, Richards D. 1992. Genetic improvement of floriculturalcrops using biotechnology. Aust J Bot 40:765-787.Ishida Y, Saito H, Ohta S, Hiei Y, Komari T, Kumashiro T.1996. High efficiency transformation of maize mediatedby Agrobacterium tumefaciens. Nature Biotechnol 14:745-750.Jefferson RA. 1987. Assaying chimeric genes in plants: the genefusion systems. Plant Mol Biol Reporter 5(4):384-405.
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