Total polyphenols analysis of mature seeds and tissue cultures of some Colombian coca varieties

  • Luisa F. Rojas Universidad de Antioquia
  • Julián Londoño Universidad de Antioquia
  • Adriana Gallego Universidad de Antioquia
  • Andrea Herrera Universidad de Antioquia
  • Carolina Aguilera Universidad de Antioquia
  • Lucía Atehortúa Universidad de Antioquia
Palabras clave: plant biotechnology, polyphenols, secondary metabolites, Theobroma cacao


The aim of this research to establish cocoa ( Theobroma cacao) cell suspensions culture to analyze the total polypenols content for two Colombian cocoa varieties and to compare the result with the total polyphenols content from the same field varieties. The final results showed that it is possible to produce big amount of cocoa cell biomass able to synthesize the metabolites without loosing its organoleptic properties (smell, color, and flavor), and to produce an acceptable content of total polyphenols compared with the natural seeds. This peliminary study is a promising perspective for future production of the antioxidants and to supplement with them cocoa by-products.
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