Identificación de Candida glabrata y otras especies comunes del género Candida mediante el uso secuencial del medio de cultivo cromógeno y la prueba del tubo germinal

Johan Sebastián Hernández-Botero; Jorge Enrique Pérez-Cárdenas

doi:10.17533/udea.iatreia.v28n4a01

Authors

Johan Sebastián Hernández-Botero Caldas University. Autonomous University of Manizales
Jorge Enrique Pérez-Cárdenas University of Caldas

DOI:

https://doi.org/10.17533/udea.iatreia.v28n4a01

Keywords:

candida glabrata, candida albicans, candida tropicalis, species identification

Abstract

Introduction: Chromogenic agar and germ tube test have been used for decades to identify C. albicans. However, neither of these tests separately allows identification of C. glabrata.

Objective: To test the efficacy of chromogenic agar and germ tube test used serially to identify the most common Candida species found in clinical practice, with emphasis on C. glabrata.

Methods: 436 isolates were identified using the chromogenic medium followed by the germ tube test. Biochemical tests were used as gold standard. Sensitivity, specificity and confidence intervals (95 %) of the serial identification system were determined.

Results: Sensitivity was 97.9 % (IC95 %: 96.0-99.9) to identify C. albicans/dubliniensis, and 90.9 % (IC95 %: 84.0-97.8) for C. tropicalis; specificity was 100 % for both species. Sensitivity was 92.5 % (IC95 %: 86.2-98.8) for identification of C. glabrata with 96.6 % (IC95 %: 95.0- 98.6) specificity. Concerning identification of the C. parapsilosis complex, specificity was 96.3 % (IC95 %: 94.5-98.1).

Conclusion: The proposed serial scheme has adequate sensitivity and specificity for identification of C. albicans/dubliniensis, C. tropicalis and C. glabrata. It could be useful in low-resources clinical settings.

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Author Biographies

Johan Sebastián Hernández-Botero, Caldas University. Autonomous University of Manizales

Surgeon, Master in Biomedical Sciences. Professor of the Medicine Program, Faculty of Health Sciences, University of Caldas and of the Medicine Program, Faculty of Health Sciences, Manizales, Colombia. Department of Basic Biological Sciences, Autonomous University of Manizales, Colombia.

Jorge Enrique Pérez-Cárdenas, University of Caldas

Associate Professor. Faculty of Health Sciences, Microbiology and Immunology Area, Department of Basic Health Sciences, University of Caldas, Manizales, Colombia.

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