Speciation of iron, nickel and cobalt in the anaerobic biodegradation of rice straw

Keywords: Biogas, metals, mineral, productivity, waste treatment


The bioavailability of Fe, Ni and Co and its impact on the mono-digestion of rice straw with addition of a natural nutrient source were assessed. The chemical forms and the degree of bioavailability of Fe, Ni and Co were investigated in two reactors during 311 days of experimentation at 37°C. The reactor (R2) was supplemented with 1 g/L of mineral once a week. The control reactor R1 was limited in mineral during the study. The greatest methane yield of 238 mL/g VS was obtained with mineral supply with an increment of 45% respect to reactor without metals (164 mL/g VS). The sequential extraction confirmed that 70, 88 and 75% of Fe, Ni and Co were mainly in bioavailable forms (interchangeable and carbonate), respectively, with mineral supply in R2. Fe and Co were mainly associated to the carbonate and oxidizable fractions (43% and 41% respectively), while the Ni concentrations were not detected during the experimentation. As a result, the methane yield in R2 increased by 45%, with a potential bioavailability (Ni > Co > Fe) higher compared to control reactor (Co > Fe). Trace elements deficit in R1 result in the reduction of metal-binding forms until the end of experiment, with a negative impact on methanogenic community.

= 240 veces | PDF
= 201 veces|


Download data is not yet available.

Author Biographies

Aimeé González Suárez, Universidad Tecnológica de La Habana "José Antonio Echeverría" (Cujae)

 Professor, Center for Process Engineering Studies (CIPRO)

Ileana Pereda Reyes, Universidad Tecnológica de La Habana "José Antonio Echeverría" (Cujae)

 Professor at the Engineering Process Centre, Research Director of CUJAE


Deny Oliva Merencio, Universidad Tecnológica de La Habana "José Antonio Echeverría" (Cujae)

Full Professor at the Renewable Energy Center, Vicerrector CUJAE

Silvio Jacinto Montalvo Martínez, Universidad Santiago de Chile

Chemical Engineering professor, Environmental Biotechnology Laboratory


C. Sawatdeenarunat, K. Surendra, D. Takara, H. Oechsner, and S. Khanal, “Anaerobic digestion of lignocellulosic biomass: Challenges and opportunities,” Bioresource Technology, vol. 178, february 2015. [Online]. Available: https://doi.org/10.1016/j.biortech.2014.09.103

I. Nges, B. Wang, Z. Cui, and J. Liu, “Digestate liquor recycle in minimal nutrients-supplemented anaerobic digestion of wheat straw,” Biochemical Engineering Journal, vol. 94, february 15 2015. [Online]. Available: https://doi.org/10.1016/j.bej.2014.11.023

T. Schmidt, M. Nelles, F. Scholwin, and J. Pröter, “Trace element supplementation in the biogas production from wheat stillage – optimization of metal dosing,” Bioresource Technology, vol. 168, september 2014. [Online]. Available: https://doi.org/10.1016/j.biortech.2014.02.124

W. Zhang, L. Zhang, and A. Li, “Enhanced anaerobic digestion of food waste by trace metal elements supplementation and reduced metals dosage by green chelating agent [S, S]-EDDS via improving metals bioavailability,” Water Research, vol. 84, november 1 2015. [Online]. Available: https://doi.org/10.1016/j.watres.2015.07.010

F. Fermoso, J. Bartacek, S. Jansen, and P. Lens, “Metal supplementation to uasb bioreactors: from cell-metal interactions to full-scale application,” Science of The Total Environment, vol. 407, june 1 2009. [Online]. Available: https://doi.org/10.1016/j.scitotenv.2008.10.043

Z. Lei, J. Chen, Z. Zhang, and N. Sugiura, “Methane production from rice straw with acclimated anaerobic sludge: Effect of phosphate supplementation,” Bioresource Technology, vol. 101, no. 12, june 2010. [Online]. Available: https://doi.org/10.1016/j.biortech.2010.01.083

K. Khatri and et al, “Synergistic effect of alkaline pretreatment and fe dosing on batch anaerobic digestion of maize straw,” Applied Energy, vol. 158, november 15 2015. [Online]. Available: https://doi.org/10.1016/j.apenergy.2015.08.045

W. Zhong and et al, “Effect of biological pretreatments in enhancing corn straw biogas production,” Bioresource Technology, vol. 102, no. 24, december 2011. [Online]. Available: https://doi.org/10.1016/j.biortech.2011.09.077

K. Pilarski and et al, “The impact of extrusion on the biogas and biomethane yield of plant substrates,” Journal of Ecological Engineering, vol. 17, no. 4, september 2016. [Online]. Available: https://doi.org/10.12911/22998993/64563

B. Evranos and B. Demirel, “The impact of Ni, Co and Mo supplementation on methane yield from anaerobic mono-digestion of maize silage,” enviromental technology, vol. 36, no. 9-12, mayjune 2016. [Online]. Available: https://doi.org/10.1080/09593330.2014.997297

H. Pobeheim, B. Munk, H.Lindorfer, and G. Guebitz, “Impact of nickel and cobalt on biogas production and process stability during semi-continuous anaerobic fermentation of a model substrate for maize silage,” Water Research, vol. 45, no. 2, january 2011. [Online]. Available: https://doi.org/10.1016/j.watres.2010.09.001

A. Suárez, K. Nielsen, S. Köhler, D. MerencioIII, and I. Reyes, “Enhancement of anaerobic digestion of microcrystalline cellulose (mcc) using natural micronutrient sources,” Brazilian Journal of Chemical Engineering, vol. 31, no. 2, april/june 2014. [Online]. Available: http://dx.doi.org/10.1590/0104-6632.20140312s00002689

A. González and et al, “Effect of natural mineral on methane production and process stability during semi-continuous monodigestion of maize straw,” Applied Biochemistry and Biotechnology, vol. 178, no. 8, april 2016. [Online]. Available: http://dx.doi.org/10.1007/s12010-015-1965-8

Fermentation of organic materials - Characterization of the substrate, sampling, collection of material data, fermentation tests, VDI, 2016.

A. González, I. Pereda, D. Oliva, T. Suárez, A. da Silva, and M. Zaiat, “Bioavailability and dosing strategies of mineral in anaerobic monodigestion of maize straw,” Engineering in Life Sciences, vol. 18, no. 8, august 2018. [Online]. Available: https: //doi.org/10.1002/elsc.201700018

M. Begoña, E. van, M. Zandvoorta, J. Izab, and P. Lens, “Effect of cobalt sorption on metal fractionation in anaerobic granular sludge,” Journal of Environmental Quality Abstract, vol. 33, no. 4, july-august 2004. [Online]. Available: https://doi.org/10.2134/jeq2004.1256

A. Tessier, P. Campbell, and M. Bisson, “Sequential extraction procedure for the speciation of particulate trace metals,” Analytical Chemistry, vol. 51, no. 7, june 1 1979. [Online]. Available: https://doi.org/10.1021/ac50043a017

L. H. and et al, “The valuation of malnutrition in the monodigestion of maize silage by anaerobic batch tests,” Water Science and Technology, vol. 58, no. 7, october 2008. [Online]. Available: https://doi.org/10.2166/wst.2008.491

C. L. and et al, “Improving biomethane production and mass bioconversion of corn stover anaerobic digestion by adding naoh pretreatment and trace elements,” Journal of Biomedicine and Biotechnology, vol. 2015, no. 13, july 2015. [Online]. Available: https://doi.org/10.1155/2015/125241

M. Ortner, M. Ramedera, L. Rachbauer, G. Bochmann, and W. Fuchs, “Bioavailability of essential trace elements and their impact on anaerobic digestion of slaughterhouse waste,” Biochemical Engineering Journal, vol. 99, july 15 2015. [Online]. Available: https://doi.org/10.1016/j.bej.2015.03.021

J. Moestedt and et al, “Effects of trace element addition on process stability during anaerobic co-digestion of ofmsw and slaughterhouse waste,” Waste Management, vol. 47, no. Part A, january 2016. [Online]. Available: https://doi.org/10.1016/j.wasman.2015.03.007

B. Demirel and P.Scherer, “Trace element requirements of agricultural biogas digesters during biological conversion of renewable biomass to methane,” Biomass and Bioenergy, vol. 35, no. 3, march 2011. [Online]. Available: https://doi.org/10.1016/j.biombioe.2010.12.022

W. Mussoline, G. Espositoa, P. Lens, A. Spagnic, and A. Giordano, “Enhanced methane production from rice straw co-digested with anaerobic sludge from pulp and paper mill treatment process,” Bioresource Technology, vol. 148, november 2013. [Online]. Available: https://doi.org/10.1016/j.biortech.2013.08.107

S. Menardo, V. Cacciatore, and P. Balsari, “Batch and continuous biogas production arising from feed varying in rice straw volumes following pre-treatment with extrusion”, Bioresource Technology, vol. 180, march 2015. [Online]. Available: http://dx.doi.org/10.1016/j.biortech.2014.12.104

R. Zhao and et al., “Methane production from rice straw pretreated by a mixture of acetic–propionic acid”, Bioresource Technology, vol. 101, no. 3, February 2010. [Online]. Available: http://dx.doi.org/10.1016/j.biortech.2009.09.020

L. M. Contreras, H. Schelle, C. Sebrango, and I. Pereda, “Methane potential and biodegradability of rice straw, rice husk and rice residues from the drying process”, Water Science and Technology, vol. 65, no. 6, march 2012. [Online]. Available: http://dx.doi.org/10.2166/wst.2012.951

V. Facchin and et al, “Effect of trace element supplementation on the mesophilic anaerobic digestion of foodwaste in batch trials: The influence of inoculums origin”, Biochemistry Engineering Journal, vol. 70, january 15 2013. [Online]. Available: http://dx.doi.org/10.1016/j.bej.2012.10.004

M. H. Zandvoort, E. D. Van Hullebusch, F. Fermoso, and P. N. Lens, “Trace metals in anaerobic granular sludge reactors: Bioavailability and dosing strategies”, Engineering Life in Science, vol. 6 no. 3, june 2006. [Online]. Available: http://dx.doi.org/10.1002/elsc.200620129

A. Van der Veen, F. Fermoso, and P. N. Lens, “Bonding form analysis of metals and sulphur fractionation in methanol-grown anaerobic granular sludge”, Engineering in Life Sciences, vol. 7, no. 5, october 2007. [Online]. Available: http://dx.doi.org/10.1002/elsc.200720208

J. Gustavsson, S. Yekta, A. Karlsson, U. Skyllberg, and B. Svensson, “Potential bioavailability and chemical forms of Co and Ni in the biogas process - An evaluation based on sequential and acid volatile sulfide extractions”, Engineering in Life Sciences, vol. 13, no. 6, november 2013. [Online]. Available: http://dx.doi.org/10.1002/elsc.201200162

How to Cite
González Suárez A., Pereda Reyes I., Oliva Merencio D., & Montalvo Martínez S. J. (2020). Speciation of iron, nickel and cobalt in the anaerobic biodegradation of rice straw . Revista Facultad De Ingeniería Universidad De Antioquia, (101), 55-63. https://doi.org/10.17533/udea.redin.20200366