Toxic effect evaluation of the lead acetate and chromium chloride on anaerobic bacterial metabolism

  • Beatriz Wills Universidad de Antioquia
  • Fernando Naranjo Universidad de Antioquia
Keywords: toxicity, inhibition, anaerobic digestion, UASB, heavy metal, methanogenesis


The toxicity of trivalent chromium and lead to anaerobic sludge system was studied. Performed assay was tested in 60 mL serum vials, the toxicity test to succeed in two steps, first with formic acid and then whey powder as a substrate. Anaerobic toxicity assays were performed taking into account, methane gas production and oxidation-reduction potential (ORP) rate, these tests were considered a useful indicator for monitoring a anaerobic sludge suffering from toxicants, over 72 hours with metals dosage against the control. First of all, exploratory assays in order to know different chromium and lead concentrations were carried out. The assays degradation activity reduced methane by 50% that came into contact with 3.322 mg Cr3+/L and 1.415 mg Pb/L and 2.291 mg Cr3+/L and 1.982 mg Pb/L with formic acid and whey powder as a substrate respectively. Heavy metal concentrations that caused 50% inhibition of methanogenesis during whey and formic acid methanation indicated that toxicity decreased in order Pb>>Cr.

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Author Biographies

Beatriz Wills, Universidad de Antioquia

Ingeniería Sanitaria y Ambiental. Grupo de Ingeniería y Gestión Ambiental, GIGA.

Fernando Naranjo, Universidad de Antioquia

Estudiante de la Maestría en Ingeniería Ambiental. Facultad de Ingeniería. 


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How to Cite
Wills B., & Naranjo F. (2004). Toxic effect evaluation of the lead acetate and chromium chloride on anaerobic bacterial metabolism. Revista Facultad De Ingeniería Universidad De Antioquia, (32), 17-25. Retrieved from