Chitosan/hydroxyapatite scaffolds for tissue engineering manufacturing method effect comparison

Diana Marcela Escobar-Sierra; Johan Martins; Claudia Patricia Ossa-Orozco

doi:10.17533/udea.redin.n75a04

Authors

Diana Marcela Escobar-Sierra University of Antioquia https://orcid.org/0000-0002-6013-7039
Johan Martins University of Franche-Comte
Claudia Patricia Ossa-Orozco University of Antioquia

DOI:

https://doi.org/10.17533/udea.redin.n75a04

Keywords:

chitosan, hydroxyapatite in situ, hydroxyapatite in powder, scaffolds chitosan/ hydroxyapatite, tissue engineering, bone

Abstract

The regeneration of bone is one of the main challenges of modern medicine because many diseases including trauma and tumor can cause bone defects. Tissue engineering (TE) is a promising approach to cure these bone diseases since it allows the reconstruction of tissue by colonization and proliferation of a patient is healthy cells is in an artificial extracellular matrix (scaffolds). The aim of this project was to prepare chitosan/hydroxyapatite CH/HA scaffolds, using various ratios and two different methods: powder hydroxyapatite (commercial) and in situ hydroxyapatite, and then compare their properties. The morphology, chemical composition, and mechanical properties were evaluated by Scanning Electron Microscopy (SEM), X-ray diffraction (XRD), Raman spectroscopy, and compression tests. The scaffolds obtained showed an interconnected porous structure. Scaffolds with better applications in tissue engineering are the scaffolds with chitosan and hydroxyapatite in situ protocol, due to their better morphology; they allow the cell growth. These scaffolds meet the requirements of tissue engineering.

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Author Biographies

Diana Marcela Escobar-Sierra, University of Antioquia

Professor Bioengineering Program, Faculty of Engineering. Researcher Biomaterials Research Group (BIOMAT).

Johan Martins, University of Franche-Comte

Franche-Comte Higher Institute of Engineering.

Claudia Patricia Ossa-Orozco, University of Antioquia

Professor of the Bioengineering Program, Faculty of Engineering. Researcher of the Biomaterials Research Group (BIOMAT).

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