The effect of hank’s balanced salt solution (HBSS) storage time on transdentinal diffusion in extracted third molars

  • Francisco Araya Universidad de Chile
  • Claudia Sommariva Universidad de Chile
  • Gustavo Moncada Universidad de Chile
  • Álvaro Cartagena Universidad de Chile
  • Claudia Letelier Universidad de Chile
  • Osmir Oliveira-Junior UNESP Araraquara
  • Javier Martín Universidad de Chile
  • Eduardo Fernández Universidad de Chile
Keywords: Dentin, Conductance, Diffusion

Abstract

Introduction: the solution used to store dentin disks while being studied has proven to be vital in reproducing possible “in vivo” conditions, and it is ultimately critical in evaluating hydraulic conductance studies. The goal of this in vitro study was to determine transdentin filtration rate variation in human dentin disks after 2, 4, 6, 7, 14, 21, and 28 days stored in Hank’s Balanced Salt Solution (HBSS), 10% formalin, and saline solution. Methods: this study included 41 unerupted healthy human third molars. These teeth were disinfected in 0.1% thymol for 24 hours before being embedded in epoxy resin blocks and then cut to obtain 1 mm thick dentin disks. The disks’ hydraulic conductance was later measured, and then they were separated and stored in different solutions. They were sorted out in three groups: a) 10% formalin, b) Hank solution (HBSS) and c) saline solution. Hydraulic conductance was measured after 1, 2, 4, 6, 7, 14, 21, and 28 days. Statistical analysis was performed by ANOVA and Tukey’s post hoc (SPSS v.15). Results: the results suggest that the average of dentine disks’ hydraulic conductance increased in all the storage solutions when comparing the analysis between day 1 and day 28. Conclusions: no significant differences were found (p > 0.005) in terms of hydraulic conductance among the various times the disks were stored in Hank’s solution.

|Abstract
= 40 veces | PDF (ESPAÑOL (ESPAÑA))
= 11 veces|

Downloads

Download data is not yet available.

Author Biographies

Francisco Araya, Universidad de Chile

Dental Surgeon, Universidad de Chile

Claudia Sommariva, Universidad de Chile
Assistant Professor, Department of Restorative Dentistry, Universidad de Chile.
Gustavo Moncada, Universidad de Chile

Professor, Department of Restorative Dentistry, Universidad de Chile

Álvaro Cartagena, Universidad de Chile
Instructors, Department of Restorative Dentistry, Universidad de Chile.
Claudia Letelier, Universidad de Chile
Instructors, Department of Restorative Dentistry, Universidad de Chile.
Osmir Oliveira-Junior, UNESP Araraquara
Professor, Department of Restorative Dentistry, Cosmetic Dentistry Studies, Universidade Estadual Paulista, São Paulo, Brasil
Javier Martín, Universidad de Chile

Assistant Professor, Department of Restorative Dentistry, Universidad de Chile 

Eduardo Fernández, Universidad de Chile

Assistant Professor, Department of Restorative Dentistry, Universidad de Chile 

References

Reeder OW Jr, Walton RE, Livingston MJ, Pashley DH. Dentin permeability: determinants of hydraulic conductance. J Dent Res 1978; 57(2): 187-193

Maita E, Simpson MD, Tao L, Pashley DH. Fluid and protein flux across the pulpodentine complex of the dog in vivo. Arch Oral Biol 1991; 36(2): 103-110.

Pashley DH, Andringa HJ, Derkson GD, Derkson ME, Kalathoor SR. Regional variability in the permeability of human dentine. Arch Oral Biol 1987; 32(7): 519-523.

Pashley DH, Nelson R, Williams EC. Dentin hydraulic conductance: changes produced by red blood cells. J Dent Res 1981; 60(10): 1797-1802.

Pashley DH, Michelich V, Kehl T. Dentin permeability: effects of smear layer removal. J Prosthet Dent 1981; 46(5): 531-537.

Pashley DH, Thompson SM, Stewart FP. Dentin permeability: effects of temperature on hydraulic conductance. J Dent Res 1983; 62(9): 956-959.

Derkson GD, Pashley DH, Derkson ME. Microleakage measurement of selected restorative materials: a new in vitro method. J Prosthet Dent 1986; 56(4): 435-440.

Pashley DH. Dentin permeability, dentin sensitivity, and treatment through tubule occlusion. J Endod 1986; 12(10): 465-474.

Pashley DH Depew DD. Effects of the smear layer, Copalite, and oxalate on microleakage. Oper Dent 1986; 11(3): 95-102.

Pashley EL, Tao L, Derkson G, Pashley DH. Dentin permeability and bond strengths after various surface treatments. Dent Mater 1989; 5(6): 375-378.

Pashley DH. Mechanisms of dentin sensitivity. Dent Clin North Am 1990; 34(3): 449-473.

Pashley DH. Dentine permeability and its role in the pathobiology of dentine sensitivity. Arch Oral Biol 1994; 39 Supl: 73S-80S.

Gillam DG, Mordan NJ, Newman HN. The Dentin Disc surface: a plausible model for dentin physiology and dentin sensitivity evaluation. Adv Dent Res 1997; 11(4): 487-501.

Pashley DH, Livingston MJ, Reeder OW, Horner J. Effects of the degree of tubule occlusion on the permeability of human dentine in vitro. Arch Oral Biol 1978; 23(12): 1127-1133.

Lanza CR, de Souza Costa CA, Furlan M, Alécio A, Hebling J. Transdentinal diffusion and cytotoxicity of self-etching adhesive systems. Cell Biol Toxicol 2009; 25(6): 533-543.

Lessa FC, Nogueira I, Huck C, Hebling J, Costa CA. Transdentinal cytotoxic effects of different concentrations of chlorhexidine gel applied on acid-conditioned dentin substrate. J Biomed Mater Res B Appl Biomater 2010; 92(1): 40-47.

Lessa FC, Nogueira I, Vargas Fda S, Spolidorio DM, Hebling J, Garcia-Godoy F, Costa CA. Direct and transdentinal antibacterial activity of chlorhexidine. Am J Dent 2010; 23(5): 255-259.

Soares DG, Ribeiro AP, Sacono NT, Coldebella CR, Hebling J, Costa CA. Transenamel and transdentinal cytotoxicity of carbamide peroxide bleaching gels on odontoblast-like MDPC-23 cells. Int Endod J 2011; 44(2): 116-125.

Turrioni AP, de Oliveira CF, Basso FG, Moriyama LT, Kurachi C, Hebling J et al. Correlation between light transmission and permeability of human dentin. Lasers Med Sci 2012; 27(1): 191-196.

Lima AF, Lessa FC, Mancini MN, Hebling J, Costa CA, Marchi GM. Transdentinal protective role of sodium ascorbate against the cytopathic effects of H2O2 released from bleaching agents. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010; 109(4): e70-76.

Thomas T, Gopikrishna V, Kandaswamy D. Comparative evaluation of maintenance of cell viability of an experimental transport media “coconut water” with Hank’s balanced salt solution and milk, for transportation of an avulsed tooth: An in vitro cell culture study. J Conserv Dent 2008; 11(1): 22-29.

Cummins D. Dentin hypersensitivity: from diagnosis to a breakthrough therapy for everyday sensitivity relief. J Clin Dent 2009; 20(1): 1-9.

Habelitz S, Marshall GW Jr, Balooch M, Marshall SJ. Nanoindentation and storage of teeth. J Biomech 2002; 35(7): 995-998.

Anjum A, Otsuki M, Matin K, Tagami J. Preservation in the liquid media produces alterations in enamel surface properties. J Dent 2009; 37(11): 884-890.

De Souza BD, Bortoluzzi EA, da Silveira Teixeira C, Felippe WT, Simões CM, Felippe MC. Effect of HBSS storage time on human periodontal ligament fibroblast viability. Dent Traumatol 2010; 26(6): 481-483.

Blomlöf L. Milk and saliva as possible storage media for traumatically exarticulated teeth. Swed Dent J Supl 1981; 8: 1-26.

Lindskog S, Blomlöf L, Hammarström L. Mitoses and microorganisms in the periodontal membrane after storage in milk or saliva. Scand J Dent Res 1983; 91(6): 465-472.

Goodis HE, Marshall GW Jr, White JM. The effects of storage after extraction of the teeth on human dentine permeability in vitro. Arch Oral Biol 1991; 36(8): 561-566.

Gopikrishna V, Baweja PS, Venkateshbabu N, Thomas T, Kandaswamy D. Comparison of coconut water, propolis, HBSS, and milk on PDL cell survival. J Endod 2008; 34(5): 587-589.

Ashkenazi M, Marouni M, Sarnat H. In vitro viability, mitogenicity and clonogenic capacity of periodontal ligament cells after storage in four at room temperature. Endod Dent Traumatol 2000; 16(2): 63-70.

Outhwaite WC, McKenzie DM, Pashley DH. A versatile split-chamber device for studying dentin permeability. J Dent Res 1974; 53(6): 1503.

Ozok AR, Wu MK, Wesselink PR. Comparison of the in vitro permeability of human dentine according to the dentinal region and the composition of the simulated dentinal fluid. J Dent 2002; 30(2-3): 107-111.

Schneider CA, Rasband WS, Eliceiri KW. NIH Image to ImageJ: 25 years of image analysis. Nat Methods 2012; 9(7): 671-675.

Sekimoto T, Derkson GD, Richardson AS. Effect of cutting instruments on permeability and morphology of the dentin surface. Oper Dent 1999; 24(3): 130-136.

Ciucchi B, Bouillaguet S, Holz J, Pashley D. Dentinal fluid dynamics in human teeth, in vivo. J Endod 1995; 21(4): 191-194.

Greenhill JD, Pashley DH. The effects of desensitizing agents on the hydraulic conductance of human dentin in vitro. J Dent Res 1981; 60(3): 686-698.

Tagami J, Tao L, Pashley DH, Hosoda H, Sano H. Effects of high-speed cutting on dentin permeability and bonding. Dent Mater 1991; 7(4): 234-239.

Camps J, Martin P, Ladeque P, Rieu R, Fuseri J. Influence of tooth cryopreservation on human dentin permeability, in vitro. Dent Mater 1994; 10(3): 210-214.

Published
2014-01-14
How to Cite
Araya F., Sommariva C., Moncada G., Cartagena Álvaro, Letelier C., Oliveira-Junior O., Martín J., & Fernández E. (2014). The effect of hank’s balanced salt solution (HBSS) storage time on transdentinal diffusion in extracted third molars. Revista Facultad De Odontología Universidad De Antioquia, 25(1), 158-175. Retrieved from https://revistas.udea.edu.co/index.php/odont/article/view/14102