Antibacterial activity of silver nanoparticles immobilized in zinc oxide-eugenol cement against Enterococcus Faecalis: an in vitro study


  • Juan Camilo Duque-Aristizábal Universidad CES
  • Liza María Isaza-Areiza Universidad CES
  • Diego Tobón-Calle Universidad CES
  • Martha Elena Londoño-López Universidad EIA



Endodontics, Sealing, Cements, Microorganisms, Enterococcus faecalis, Kirby-Bauer, Nanotechnology, In-vitro


Introduction: the aim of this study is to evaluate the antimicrobial capacity of silver nanoparticles immobilized in a zinc oxide-eugenol (ZOE) cement against Enterococcus faecalis for potential use in endodontic treatments. Method: experimental in vitro study, performing synthesis of silver nanoparticles (AgNPs) and UV-visible spectroscopy to confirm presence of AgNPs in the prepared solutions. The ZOE mixture was standardized, producing the integrated AgNPs/ZOE material. Scanning electron microscopy (SEM) and Fourier-Transform Infrared Spectroscopy (FTIR) were used to characterize the integrated material; a KirbyBauer assay was finally run to measure the inhibition halos produced by the compound-microorganism interaction. Results: the UV-visible spectroscopy showed presence of AgNPs in the created solution; both SEM and FTIR show that the AgNPs are integrated into the ZOE system, not altering their properties when performed under conditions like those found in the mouth. The Kirby-Bauer assay shows that all samples had inhibition halos. The AgNPs in guava extract had statistically significant differences with the halos of the other samples (p < 0.05). Conclusions: the obtained AgNPs show bactericidal activity against Enterococcus faecalis, as a statistically significant difference was found between the AgNPs suspended in guava extract and the other groups; this will be the starting point for future research

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Author Biographies

Juan Camilo Duque-Aristizábal, Universidad CES

DDS, Endodontics Specialist, Master of Dental Sciences, Universidad CES

Liza María Isaza-Areiza, Universidad CES

DDS, Endodontics Specialist, Master of Dental Sciences, Universidad CES

Diego Tobón-Calle, Universidad CES

DDS, CES Endodontics Specialist, Head of Graduate Endodontics Studies, Universidad CES

Martha Elena Londoño-López, Universidad EIA

Physicist, Doctor of Science and Materials Technology, Universidad EIA


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How to Cite

Duque-Aristizábal, J. C., Isaza-Areiza, L. M., Tobón-Calle, D., & Londoño-López, M. E. (2019). Antibacterial activity of silver nanoparticles immobilized in zinc oxide-eugenol cement against Enterococcus Faecalis: an in vitro study. Revista Facultad De Odontología Universidad De Antioquia, 30(2), 154–165.