Effect of Aloe vera and vitamin E supplementation on the immune response of broilers


  • Babak Darabighane University of Mohaghegh Ardabili
  • Ali Mahdavi Semnan University
  • Farzad Mirzaei Aghjehgheshlagh University of Mohaghegh Ardabili
  • Abolfazl Zarei Islamic Azad University
  • Eleni Kasapidou Technological Educational Institute of Western Macedonia
  • Samuel N. Nahashon Tennessee State University




antibiotic, immune system, medicinal plant, poultry


Background: herbs are among the feed supplements under investigation that could replace antibiotics to improve humoral and cellular immune response in birds. Objective: this study evaluated the potential of Aloe vera (Aloe barbadensis Miller) and vitamin E as immunostimulants on humoral and cellular immune responses in broilers. Methods: broilers were randomly assigned to three dietary treatments: a negative control (basal diet + with no additive), basal diet + 1% Aloe vera gel in drinking water, and basal diet + 100 mg/Kg vitamin E in the feed. Antibody titers against sheep red blood cells and Newcastle disease virus were used to examine the humoral immune response, whereas cellular immune response was evaluated using the phytohemagglutinin-P tests. Results: the highest level of antibody titer against sheep red blood cells on examination days 28 and 38, and the highest response to injection of phytohemagglutinin-P on day 38 was observed in the Aloe vera gel group (p<0.05). However, the response of broilers fed Aloe vera gel was not different from those receiving vitamin E (p>0.05). In addition, the greatest antibody level against Newcastle disease virus was obtained on days 25 and 35 in the vitamin E group, with no significant difference from the Aloe vera gel group (p>0.05). Conclusion: in general, our findings demonstrated that both Aloe vera gel and vitamin E can enhance humoral and cellular immune responses of broilers, while Aloe vera gel can be used as an immunostimulant in chickens.

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Author Biographies

Babak Darabighane, University of Mohaghegh Ardabili

MSc., Department of Animal Science, University of Mohaghegh Ardabili, Ardabil, Iran.

Ali Mahdavi, Semnan University

PhD., Faculty of Veterinary Medicine, Semnan University.

Farzad Mirzaei Aghjehgheshlagh, University of Mohaghegh Ardabili

PhD., Department of Animal Science, University of Mohaghegh Ardabili, Ardabil, Iran.

Abolfazl Zarei, Islamic Azad University

PhD., Department of Animal Science, Karaj Branch, Islamic Azad University, Karaj, Iran.

Eleni Kasapidou, Technological Educational Institute of Western Macedonia

PhD., Department of Agricultural Technology, Division of Agricultural Products Quality Control, School of Agriculture Technology, Food Technology and Nutrition, Technological Educational Institute of Western Macedonia, Florina, Greece.

Samuel N. Nahashon, Tennessee State University

PhD., Department of Agricultural Sciences, Tennessee State University, Nashville, USA.


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How to Cite

Darabighane, B., Mahdavi, A., Aghjehgheshlagh, F. M., Zarei, A., Kasapidou, E., & Nahashon, S. N. (2017). Effect of Aloe vera and vitamin E supplementation on the immune response of broilers. Revista Colombiana De Ciencias Pecuarias, 30(2), 159–164. https://doi.org/10.17533/udea.rccp.v30n2a07



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