Tissue fibrosis and its correlation with malignancy in canine mammary tumors
DOI:
https://doi.org/10.17533/udea.rccp.v31n4a06Keywords:
biological biomarkers, breast cancer, dogs, histological stain, histopathology, Masson’s trichromeAbstract
Background: Fibrosis is present in several pathologies associated with mammary carcinogenesis. Objective: To evaluate and quantify the fibrosis present in malignant and benign mammary neoplasms in bitches. Methods: Eighty-three samples were divided according to histopathological diagnosis into benign (n= 21) and malignant (n= 62) neoplasms. Haematoxylin-eosin and Masson’s trichrome were used to locate the connective tissue, and the extent of fibrosis was assessed with image software. Results: Benign neoplasms were classified into adenomas (cystic, complex, and tubular), benign mixed tumor, and ductal and lobular hyperplasia. Malignant neoplasms were classified as carcinomas (complex, mixed tumor, in situ tubular, tubulopapillary, and solid). Grade I was the most prevalent histopathological class, followed by grade II and III. Fibrosis was classified as severe, moderate, or discrete. No significant (p>0.05) difference was observed for the percentage of fibrosis between malignant and benign group neoplasms. However, difference (p=0.028) was found for fibrosis percentage between histopathological subtypes of tumors. The benign subtype of lobular hyperplasia presented differences between cystic adenoma and benign mixed tumor. The in situ malignant tubular carcinoma subtype presented differences between solid and tubulopapillary carcinoma. Conclusions: Fibrosis in canine mammary tumors can be estimated with Massons’s trichrome staining.
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Ahn S, Cho J, Sung J, Lee JE, Nam SJ, Kim KM, Cho EY. The prognostic significance of tumor-associated stroma in invasive breast carcinoma. Tumor Biol 2012; 33:1573-1580.
Barr RG, Nakashima K, Amy D, Cosgrove D, Farrokh A, Schaffer F, Bamber JC, Castera L, Choi BI, Chou YH, Dietrich CF, Ding H, Ferraioli G, Filice C, Friedrich-Rust M, Hall TJ, Nightingale KR, Palmeri ML, Shiina T, Suzuki S, Sporea I, Wilson S, Kudo M. WFUMB guidelines and recommendations for clinical use of ultrasound elastography: Part 2: breast. Ultrasound Med Biol 2015; 41:1148-1160.
Brown E, Mckee T, Ditomaso E. Dynamic imaging of collagen and its modulation in tumors in vivo using second-harmonic generation. Nature Med 2003; 9:796-800.
Carvalho MI, Carvalho RS, Pires i, Prada J, Bianchini R, Jensen-Jarolim E, Queiroga FL. A Comparative Approach of Tumor-Associated Inflammation in Mammary Cancer between Humans and Dogs. BioMed Res Int 2016. 4917387.
Cassali GD, Lavalle GE, Nardi AB de, Ferreira E, Bertagnolli AC, Estrela-Lima A, Alessi AC, Daleck CR, Salgado BS, Fernandes CG, Sobral RA, Amorim RL, Gamba CO, Damasceno KA, Auler PA, Magalhães GM, Silva JO, Raposo JB, Ferreira AMR, Oliveira LO, Malm C, Zuccari DAPC, Tanaka NM, Ribeiro LR, Campos LC, Souza CM, Leite JS, Soares LMC, Cavalcanti MF, Fonteles ZGC, Schuch ID, Paniago J, Oliveira TS, Terra EM, Castanheira TLL, Felix AOC, Carvalho GD, Guim TN, Garrido G, Fernandes SC, Maia FCL, Dagli MLZ, Rocha NS, Fukumasu H, Grandi F, Machado JP, Silva SMMS, Bezerril JE, Frehse MS, Almeida ECP, Campos CB. Consensus for the Diagnoses, Prognosis and Treatment of Canine Mammary Tumors. Brazilian J Vet Pathol 2011; 4:153-180.
Cassali GD, Lavalle GE, Ferreira E, Estrela-Lima A, Nardi AB de, Ghever C, Sobral RA, Amorim RL, Oliveira LO, Sueiro FAR, Beserra HEO, Bertagnolli AC, Gamba CO, Damasceno KA, Campos CB, Araujo MR, Campos LC, Monteiro LN, Nunes FC, Horta RS, Reis DC, Luvizotto MCR, Magalhães GM, Raposo JB, Ferreira AMR, Tanaka NM, Grandi F, Ubukata R, Batschinski K, Terra EM, Salvador RCL, Jark P, Delecrodi JER, Nascimento AN, Silva DN, Silva LP, Ferreira KCRS, Frehse MS, Di Santis GW, Silva EO, Guim TN, Kerr B, Cintra PP, Silva FBF, Leite JS, Mello MFV, Ferreira MLG, Fukumasu H, Salgado BS, Torres R. Consensus for the Diagnosis, Prognosis and Treatment of Canine Mammary Tumors - 2013. Brazilian J Vet Pathol 2014; 7:38-46.
Cintra PP, Paula CAT, Calazans SG, Souza JL, Magalhães GM. Reclassificação e determinação do tipo histológico predominante em neoplasias mamárias caninas do Hospital Veterinário da Universidade de Franca nos anos de 2010 a 2012. Enciclopédia Biosfera, Centro Científico Conhecer 2014; 10:2067.
Elston CW, Ellis IO. Assessment of histological grade. ELSTON CW., ELLIS IO. Eds. Systemic Pathology. The breast. London: Churchill Livingstone, 1998, 365-384.
Feliciano MAR, Silva AS, Peixoto RVR, Galera PD, Vicente, WRR. Estudo clínico, histopatológico e imunoistoquímico de neoplasias mamárias em cadelas. Arq Bras Med Vet Zootec 2012; 64:1094-1100.
Goldschmidt M, Peña L, Zappulli V. Tumors of the mammary gland. In: MEUTEN, D.J. Tumors in domestic animals, 5.ed. Ames: Iowa State Press, p. 723-757, 2017.
Goldschmidt M, Peña L, Rasotto R, Zappuli V. Classification and grading of canine mammary tumors. Vet Pathol 2011; 48:117-131.
Guarino M, Tosoni A, Nebuloni M. Direct contribution of epithelium to organ fibrosis: epithelial-mesenchymal transition. Human Pathol 2009; 40:1365-76.
Hanahan DE, Weinberg RA. The hallmarks of cancer. Cell 2000; 100:57–70.
Hasebe T, Tsuda H, Hirorashi S, Shimosato Y, Iwai M, Imoto S, Mukai K. Fibrotic focus in invasive ductal carcinoma: an indicatoror high tumor aggressiveness. Jpn J Cancer Res 1996; 87:385-394.
Hasebe T, Mukai K, Tsuda H, Ochiai A. New prognostic histological parameter of invasive ductal carcinoma of the breast: clinicopathological significance of fibrotic focus. Pathol Int 2000;50:263-272.
Itoh T, Uchida K, Ishikawa K, Kushima K, Kushima E, Tamada H, Moritake T, Nakao H, Shii H. Clinicopathological survey of 101 canine mammary gland tumors: Differences between small-breeddogs and others. J Vet Med Sci 2005; 67:345-347.
Jensen-Jarolim E, Fazekas J, Singer J, Hofstetter G, Oida K, Matsuda H, Tanaka A. Crosstalk of carcino embryonic antigen and transforming growth factor-β via their receptors: comparing human and canine cancer. Cancer Immunol Immunother 2015; 64:531-537.
Kalluri R. Basement membranes: structure, assembly and role in tumor angiogenesis. Nature Rev Cancer 2003; 3:422–433.
Kalluri R, Zeisberg M. Fibroblasts in cancer. Nature Rev Cancer 2006; 6:392-40.
Klopfleisch R, Von Euler H, Sarli G, Pinho SS, Gärtner F, Gruber AD. Molecular Carcinogenesis of Canine Mammary Tumors: News From an Old Disease. Vet Pathol 2011; 48:98-116.
Lana SE, Rutteman GR, Withrow SJ, Macewen EG. Tumors of the mammary gland. In: Withrow SJ, Macewen EG. Small Animal Clinical Oncology. 5 th ed. St Louis: Elsevier Saunders, 2013, 619-636.
Liu B, Zheng Y, Huang G, Lin M, Shan Q, Lu Y, Tian W, Xie X. Breast Lesions: Quantitative Diagnosis Using Ultrasound Shear Wave Elastography - A Systematic Review and Meta-Analysis. Ultrasound Med Biol 2016; 42:835-847.
Mcphail LD, Robinson SP. Intrinsic susceptibility MR Imaging of Chemically Induced Rat Mammary Tumors: Relationship to Histologic Assessment of Hypoxia and Fibrosis. Radiology 2010;254:110-118.
Misdorp W, Else RW, Hellmén E. Histological classification of mammary tumors of the dog and the cat. Armed Forces Institute of Pathology. Washington: 1999; 7:1-59.
Moulton JE. 1990. Tumors of mammary gland. In: MOULTON, J. E (Ed) Tumors in domestic animals. 3rd ed. p. 518-552.
Nardi AB, Rodaski S, Sousa RS, Costa TA, Macedo TR, Rodigheri SM, Rios A, Piekarz CH. Prevalence of neoplasms and kind of treatments in dogs seen in Veterinary Hospital at University Federal of Paraná. Arch Vet Sci 2002; 7:15-26.
Oliveira LO, Oliveira RT, Loretti AP, Rodrigues R, Driemeier D. Aspectos epidemiológicos da neoplasia mamária canina. Acta Sci Vet 2003; 31:105-110.
Oliveira Filho JC, Kommers GD, Masuda EK, Marques BMFPP, Fighera RA, Irigoyen LF, Barros CSL. Estudo retrospectivo de 1.647 tumores mamários em cães. Pesq Vet Bras 2010; 30:177-185.
Owen LN. The TNM Classification of tumors in domestic animals. Geneva: World Health Organization; 1980.
Queiroga FL, Raposo T, Carvalho MI, Prada J, Pires I. Canine Mammary Tumours as a Model to Study Human Breast Cancer: Most Recent Findings. In vivo 2011; 25:455-466.
Solez K, Axelsen RA, Benediktsson H, Burdick JF, Cohen AH, Colvin RB et al. International standardization of criteria for the histologic diagnosis of renal allograft rejection: The Banff working classification of kidney transplant pathology. Kidney International 1993; 44:411–422.
Soler M, Dominguez E, Lucas X, Novellas R, Gomes-Coelho KV, Espada Y, Agut A. Comparison between ultrasonographic findings of benign and malignant canine mammary gland tumours using B-mode, colour Doppler, power Doppler and spectral Doppler. Res Vet Sci 2016; 107:141-146.
Sorenmo K. Canine mammary gland tumors. Vet Clin North Am Small Anim Practice 2003; 33:573-596.
Sorenmo KU, Kristiansen VM, Cofone MA, Shofer FS, Breen AM, Langeland M, Mongil CM, Grondahl AM, Teige J, Goldschmidt MH. Canine mammary gland tumors; a histological continuum from benign to malignant; clinical and histopathological evidence. Vet Comp Oncol 2009; 7:162-172.
Thannickal VJ, Toews GB, White ES, Lynch JP, Martinez FJ. Mechanisms of pulmonary fibrosis. Annu Rev Med 2004; 5:395-417.
Van Den Eynden G G, Colpaert CG, Couvelard A, Pezzella F, Dirix LY, Vermeulen PB, Van Marck EA, Hasebe T. A fibrotic focus is a prognostic factor and a surrogate marker for hypoxia and (lymph) angiogenesis in breast cancer: review of the literature and proposal on the criteria of evaluation. Histopathol 2007;51:440-451.
Visan S, Balacescu O, Berindan-Neagoe I, Catoi C. In vitro comparative models for canine and human breast cancers. Clujul Medical 2016; 89:38-49.
Wynn TA. Cellular and molecular mechanisms of fibrosis. J Pathol2008; 214:199-210.
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