Pregnancy and delivery rates after vitrification of in vitro-produced Nelore (Bos indicus) embryos under field conditions

Authors

  • Lucas C. Pereira Federal Rural University of Pernambuco
  • José C. Ferreira-Silva Federal Rural University of Pernambuco
  • Ludymila C. Cantanhêde Federal Rural University of Pernambuco
  • Sarah R. L. Basto Federal Rural University of Pernambuco
  • Humberto F. Veloso Neto Federal Rural University of Pernambuco
  • Marcelo T. Moura Federal Rural University of Pernambuco
  • Marcos Antonio L. Oliveira Federal Rural University of Pernambuco

DOI:

https://doi.org/10.17533/udea.rccp.v32n1a05

Keywords:

cattle reproduction, cryobiology, cryoinjury, cryotolerance, embryo surplus, embryo transfer

Abstract

Background: Cryopreservation preserves cellular viability under low temperatures, resulting in diminished intracellular enzymatic activity and reduced cellular metabolism that ultimately allows preserving genetic material for indefinite periods of time. Embryos submitted to cryopreservation suffer from considerable morphological and functional damage, resulting in reduced survival and development rates. Objective: To evaluate pregnancy and delivery rates of in vitro-produced (IVP) Nellore (Bos indicus) embryos after vitrification under field conditions. Methods: The IVP embryos at blastocyst (Bl) and expanded blastocyst (Bx) were transferred fresh (n= 137) or after vitrification (n= 127). Results: Pregnancy rates at 35 d for fresh embryos were lower in Bl (41.6) than in Bx (60.9) (p0.05). Pregnancy loss at 60 d were similar (p>0.05) for both fresh (Bl: 3.1 and Bx: 4.8) and vitrified embryos (Bl: 1.9 and Bx: 4.7). Delivery rates were similar between groups (p>0.05). Conclusion: Both pregnancy and delivery rates of Bos indicus IVP embryos vitrified under field conditions are indistinguishable from fresh embryos.

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Author Biographies

Lucas C. Pereira, Federal Rural University of Pernambuco

VM, MSc., Laboratory of Reproductive Biotechnics, Department of Veterinary Medicine, Federal Rural University of Pernambuco, Brazil.

José C. Ferreira-Silva, Federal Rural University of Pernambuco

VM, MSc., Laboratory of Reproductive Biotechnics, Department of Veterinary Medicine, Federal Rural University of Pernambuco, Brazil.

Ludymila C. Cantanhêde, Federal Rural University of Pernambuco

Biol., MSc., Laboratory of Reproductive Biotechnics, Department of Veterinary Medicine, Federal Rural University of Pernambuco, Brazil.

Sarah R. L. Basto, Federal Rural University of Pernambuco

Biol., MSc., Laboratory of Reproductive Biotechnics, Department of Veterinary Medicine, Federal Rural University of Pernambuco, Brazil.

Humberto F. Veloso Neto, Federal Rural University of Pernambuco

VM, MSc., Laboratory of Reproductive Biotechnics, Department of Veterinary Medicine, Federal Rural University of Pernambuco, Brazil.

Marcelo T. Moura, Federal Rural University of Pernambuco

Biol., Dr., Laboratory of Reproductive Biotechnics, Department of Veterinary Medicine, Federal Rural University of Pernambuco, Brazil.

Marcos Antonio L. Oliveira, Federal Rural University of Pernambuco

VM, PhD., Laboratory of Reproductive Biotechnics, Department of Veterinary Medicine, Federal Rural University of Pernambuco, Brazil.

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Published

2019-03-27

How to Cite

Pereira, L. C., Ferreira-Silva, J. C., Cantanhêde, L. C., Basto, S. R. L., Veloso Neto, H. F., Moura, M. T., & Oliveira, M. A. L. (2019). Pregnancy and delivery rates after vitrification of in vitro-produced Nelore (Bos indicus) embryos under field conditions. Revista Colombiana De Ciencias Pecuarias, 32(1), 43–49. https://doi.org/10.17533/udea.rccp.v32n1a05

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