Estimating biological reference intervals for cell blood counts in a dog population

  • Valentina Ochoa-Ruíz University of Antioquia
  • Víctor D. Velasco-Pérez University of Antioquia
  • Margarita Patiño TestLab Laboratory
  • Jhonatan S. Restrepo-León TestLab Laboratory
  • Jannet Zapata-Bailarín University of Antioquia
  • Nathalia M. Correa-Valencia University of Antioquia
  • Patricia E. Jaramillo-Arbeláez University of Antioquia
Keywords: Barr chromatin, biological intervals, blood, blood cells, canines, cellular count, chromatin, dog, erythroblasts, erythrogram, hematology, hemogram

Abstract

Background: The results from automated equipment and peripheral blood smears allow correlating clinical data with cellular blood counts (CBC), generating information on pathologies of hematological and non-hematological origin in dogs. Objective: To describe qualitative and quantitative magnitudes of CBC in healthy donor dogs of a blood- bank in Medellín (Colombia). Methods: A descriptive-prospective study was carried out from 146 records of CBC results from a blood-bank. The samples were processed by automated equipment, and trained personnel performed the observation of peripheral blood smears to collect qualitative information. Variables such as age, sex, breed, quantitative results, and observations of the peripheral blood smears were considered. For the definition of biological intervals (BIs), the mean and two standard deviations were used for the data with a normal distribution. Otherwise, the 2.5th and 97.5th percentiles were used. Results: The size, granularity, and cytoplasmic vacuoles in monocytes and polymorphonuclear eosinophils, as well as the presence of Barr chromatin and occasional circulating erythroblasts, were remarkable findings to the peripheral blood smear. With the standardization of data obtained from dogs of large and giant breeds, the present work provides BIs for some of the CBC data in the studied population. Conclusion: The findings of the present study allow approximations to the definition of alterations in blood cells and their counts, which can guide the veterinarian towards an early diagnosis in dogs.

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Author Biographies

Valentina Ochoa-Ruíz, University of Antioquia

https://orcid.org/0000-0003-2393-4167
School of Microbiology, University of Antioquia, Medellín, Colombia.

Víctor D. Velasco-Pérez, University of Antioquia

https://orcid.org/0000-0002-5660-4335
School of Microbiology, University of Antioquia, Medellín, Colombia.

Margarita Patiño, TestLab Laboratory

https://orcid.org/0000-0003-1597-6316
TestLab Laboratory, Medellín, Colombia.

Jhonatan S. Restrepo-León, TestLab Laboratory

https://orcid.org/0000-0002-6355-1040
TestLab Laboratory, Medellín, Colombia.

Jannet Zapata-Bailarín, University of Antioquia

https://orcid.org/0000-0001-9053-4601
School of Microbiology, University of Antioquia, Medellín, Colombia.

Nathalia M. Correa-Valencia, University of Antioquia

https://orcid.org/0000-0001-8836-8827
Centauro Research Group, School of Veterinary Medicine, Faculty of Agrarian Sciences, University of Antioquia, Medellín, Colombia.

Patricia E. Jaramillo-Arbeláez, University of Antioquia

https://orcid.org/0000-0002-6832-0276
Molecular Hematopathology Group (HEMO), School of Microbiology, University of Antioquia, Medellín, Colombia.

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Published
2020-09-25
How to Cite
Ochoa-Ruíz V., Velasco-Pérez V. D., Patiño M., Restrepo-León J. S., Zapata-Bailarín J., Correa-Valencia N. M., & Jaramillo-Arbeláez P. E. (2020). Estimating biological reference intervals for cell blood counts in a dog population. Revista Colombiana De Ciencias Pecuarias, 34(2), 126-144. https://doi.org/10.17533/udea.rccp.v34n2a07
Section
Original research articles