Oxidative stress parameters in dogs naturally infected with sarcoptic mange

Authors

  • Kerim Emre Yanar Ataturk University
  • Sefa Kucukler Ataturk University
  • Emre Eren Ataturk University
  • Muhammed Sertaç Eroglu Ataturk University
  • Murat Ilgun Ataturk University
  • Cihan Gur Ataturk University
  • Fatih Mehmet Kandemir Aksaray University
  • Mustafa Sinan Aktas

DOI:

https://doi.org/10.17533/udea.rccp.v36n4a2

Keywords:

biomarker, dog, infestation, oxidative stres, Sarcoptes, sarcoptic mange, scabies, zoonosis

Abstract

Background: Scabies is one of the most common diseases in dogs. It threatens both animals and humans due to its zoonotic potential. Objective: The purpose of this study was to evaluate the oxidant/antioxidant balance with hematological findings in dogs infested with sarcoptic mange. Methods: The animals evaluated in this study consisted of 32 mixed-breed dogs between 1 and 2 years of age. The dogs were allocated into two groups: a control group (infestation-free animals; n=10), and a sarcoptic mange-infected group (Sarcoptes, n=22). Dogs in the Sarcoptes group showed infestation signs such as intense itching, excoriations, alopecia, and blistering of the elbow and auricular margins. Results: Significant increase (p<0.01) levels were observed in total oxidant status (TOS), malondialdehyde (MDA), oxidative stress index (OSI), and nitric oxide (NO), while glutathione (GSH) and total antioxidant status (TAS) levels in dogs infested with Sarcoptes decreased significantly (p<0.01). In addition, a significant increase (p<0.01) of WBC count in dogs in the sarcoptic group in comparison with the control was found. Conversely, there was significant decrease (p<0.01) in RBC, HGB, and PCV counts in Sarcoptes-infested dogs. Conclusions: Our study suggests a possible relationship between oxidant/antioxidant imbalance and hematological findings in dogs infested with sarcoptic mange. Furthermore, in addition to MDA, TAS, TOS, and OSI markers, NO as well as GSH might be also used to assess the oxidative stress in dogs naturally infected with Sarcoptes scabiei.

|Abstract
= 288 veces | PDF
= 238 veces|

Downloads

Download data is not yet available.

Author Biographies

Kerim Emre Yanar, Ataturk University

Department of Internal Medicine, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey
https://orcid.org/0000-0001-7302-7077

Sefa Kucukler, Ataturk University

Department of Biochemistry, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey
https://orcid.org/0000-0002-8222-5515

Emre Eren, Ataturk University

Department of Internal Medicine, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey
https://orcid.org/0000-0002-2966-5466

Muhammed Sertaç Eroglu, Ataturk University

Department of Internal Medicine, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey
https://orcid.org/0000-0003-4243-8273

Murat Ilgun, Ataturk University

Department of Internal Medicine, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey
https://orcid.org/0000-0002-0062-2825

Cihan Gur, Ataturk University

Department of Biochemistry, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey
https://orcid.org/0000-0001-6775-7858

Fatih Mehmet Kandemir, Aksaray University

Department of Biochemistry, Faculty of Medicine, Aksaray University, Aksaray, Turkey
https://orcid.org/0000-0002-8490-2479

Mustafa Sinan Aktas

Department of Internal Medicine, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey
https://orcid.org/0000-0002-7206-5757

References

Aatish HU, Zia-Ud-Din S, Iqbal Z, Jabbar A, Tasawar Z. Prevalence of sheep mange in district Dera Ghazi Khan (Pakistan) and associated hematological/biochemical disturbances. IJAB 2007; 9(6):917-920.

Allaam MA, Allam TS, Elkhatam AO. Biochemical and circulating oxidative stress biomarkers in Egyptian buffaloes (Bubalus bubalis) infested by sarcoptic mange. Glob Vet 2014; 13(4):656-661.

Beigh SA, Soodan JS, Bhat AM. Sarcoptic mange in dogs: Its effect on liver, oxidative stress, trace minerals and vitamins. Vet Parasitol 2016; 227:30-34. https://doi.org/10.1016/j.vetpar.2016.07.013

Bickers DR, Athar M. Oxidative stress in the pathogenesis of skin disease. J Invest Dermatol 2006; 126(12):2565-2575. https://doi.org/10.1038/sj.jid.5700340

Cam Y, Atasever A, Erslan G, Kibar M, Atalay O, Beyaz L, Incı A, Lıman BC. Eimeria steidae: experimental infection in rabbits and the effect of treatment with toltrazuril and ivermectin. Exp Parasitol 2008; 119(1):164-172. https://doi.org/10.1016/j.exppara.2008.01.005

Camkerten I, Sahin T, Borazan G, Gokcen A, Das A. Evaluation of blood oxidant/antioxidant balance in dogs with sarcoptic mange. Vet Parasitol 2009; 161(1-2):106–109. https://doi.org/10.1016/j.vetpar.2008.12.019

Cemek M, Enginar H, Karaca T, Unak P. In vivo radio-protective effects of Nigella satival oil and reduced glutathi-one against irradiation-induced oxidative injury and number of peripheral blood lymphocytes in rats. Photochem Photobiol 2006; 82(6):1691–1696. https://doi.org/10.1111/j.1751-1097.2006.tb09832.x

Chandramathi S, Kumar S, Sinnadurai S, Kuppusamy UR. Stress exacerbates infectivity and pathogenicity of blastocystis hominis. In vitro and in vivo evidence. Plos One 2014; 9(5):e94567. https://doi.org/10.1371/journal.pone.0094567

De UK, Dey S. Evaluation of organ function and oxidant/antioxidant status in goats with sarcoptic mange. Trop Anim Health Prod 2010; 42(8):1663-1668. https://doi.org/10.1007/s11250-010-9618-y

Dimri U, Sharma MC, Swarup D, Ranjan R, Kataria M. Alterations in hepatic lipid peroxides and antioxidant profile in Indian water buffaloes suffering from sarcoptic mange. Res Vet Sci 2008; 85(1):101-105. https://doi.org/10.1016/j.rvsc.2007.07.006

Dimri U, Bandyopadhyay S, Singh SK, Ranjan R, Mukherjee R, Yatoo MI, Patra PH, De UK, Dar AA. Assay of alterations in oxidative stress markers in pigs naturally infested with Sarcoptes scabiei var. suis. Vet Parasitol 2014; 205(1-2):295-299. https://doi.org/10.1016/j.vetpar.2014.06.015

Ercan N, Fidancı UR. Urine 8-hydroxy-2’-deoxyguanosine (8-OHdG) levels of dogs in pyoderma. Vet J Ankara Univ 2012; 59:163–168.

Erel O: A new automated colorimetric method for measuring total oxidant status. Clin Biochem 2005; 38(12):1103-1111. https://doi.org/10.1016/j.clinbiochem.2005.08.008

Erel O: A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem 2004; 37(2):112-119. https://doi.org/10.1016/j.clinbiochem.2003.10.014

Espinosa J, Perez MJ, Jorge RL, R´Aezbravo A, Francisco JC, Paulino F, Soriguer CR, Enrique JG, Romero D. Evaluation of oxidant/antioxidant balance in Iberian ibex (Capra pyrenaica) experimentally infested with Sarcoptes scabiei Vet Parasitol 2017; 242:63-70. https://doi.org/10.1016/j.vetpar.2017.05.027

Hampel V, Knaus M, Schäfer J, Beugnet F, Rehbein S. Treatment of canine sarcoptic mange with afoxolaner (NexGard) and afoxolaner plus milbemycin oxime (NexGard Spectra) chewable tablets: efficacy under field conditions in Portugal and Germany. Parasite 2018; 25:63. https://doi.org/10.1051/parasite/2018064

Kaya S, Suutcu R, Cetin ES, Aridogan BC, Delibas N, Demirci M. Lipid peroxidation level and antioxidant enzyme activities in the blood of patients with acute and chronic fascioliasis. Int J Infect Dis 2007; 11(3):251-255. https://doi.org/10.1016/j.ijid.2006.05.003

Kemp DJ, Walton SF, Harumal P, Currie BJ. The scourge of scabies. Biologist 2002; 49(1):19-24.

Kleczkowski M, Kluciński W, Sikora J, Zdanowicz M, Dziekan P: Role of the antioxidants in the protection against oxidative stress in cattle--nonenzymatic mechanisms (Part 2). Pol J Vet Sci 2004; 7(3):233–240.

Knight JA: Review: free radicals, antioxidants, and the immune system. Ann Clin Lab Sci 2000; 30(2):145–158.

Kocyigit A, Keles H, Selek S, Guzel S, Celık H, Erel O: Increased DNA damage and oxidative stress in patients with cutaneous leishmaniasis. Mutat Res 2005; 585(1-2):71–78. https://doi.org/10.1016/j.mrgentox.2005.04.012

Ljunggren EL. Molecular analysis of Sarcoptes scabiei. Thesis, Swedish University of Agricultural Sciences, 47, 2005.

Mark R, Johannes B, Maria KS, Andrea T, Klaus R. Oxidative stress in aging human skin. Biomolecules 2015; 5(2):545-589. https://doi.org/10.3390/biom5020545

Mcclain D, Dana AN, Goldenberg G. Mite infestations. Dermatol Ther 2009; 22(4):327-346. https://doi.org/10.1111/j.1529-8019.2009.01245.x

Parmar AJ, Sıngh V, Chaudhary SS, Prajapati BH, Sengar YS. Hematobiochemical studies on sarcoptic mange in camel (Camelus dromedarius) in Banaskantha district (North Gujarat). J Parasitic Dis 2005; 29(1):71-73.

Paterson TE, Halliwell RE, Fields PJ, Louw ML, Ball G, Louw J, Pinckney R. Canine generalized demodicosis treated with varying doses of a 2.5% moxidectin+ 10% imidacloprid spot-on and oral ivermectin: Parasiticidal effects and long-term treatment outcomes. Vet Parasitol 2014; 205(3-4):687-696. https://doi.org/10.1016/j.vetpar.2014.08.021

Pérez JM, Serrano E, Sorıguer RC, González FJ, Sarasa M, Granados JE, Cuenca R, Fandos P. Distinguishing disease effects from environmental effects in a mountain ungulate: seasonal variation in body weight, hematology, and serum chemistry among Iberian ibex (Capra pyrenaica) affected by sarcoptic mange. J Wildl Dis 2015; 51(1):148–156. https://doi.org/10.7589/2014-01-008

Lalli PN, Morgan MS, Arlian LG. Skewed Th1/Th2 immune response to Sarcoptes scabiei. J Parasitol 2004; 90(4):711-714. https://doi.org/10.1645/GE-214R

Placer ZA, Cushman L, Johnson BC. Estimation of products of lipid peroxidation (malonyl dialdehyde) in biological fluids. Anal Biochem 1966; 16(2):359-364. https://doi.org/10.1016/0003-2697(66)90167-9

Sedlak J, Lındsay RH. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem 1968; 25(1):192-205. https://doi.org/10.1016/0003-2697(68)90092-4

Serarslan G, Yılmaz HR, Sogut S. Serum antioxidant activities, malondial dehyde and nitric oxide levels in human cutaneous leishmaniasis. Clin Exp Dermatol 2005; 30(3):267–271. https://doi.org/10.1111/j.1365-2230.2005.01758.x

Shalaby MF, El-Din AN, El-Hamd MA. Isolation, identification, and in vitro antifungal susceptibility testing of dermatophytes from clinical samples at Sohag University Hospital in Egypt. electron. Physician 2016; 8(6):2557-2567. https://doi.org/10.19082/2557

Stevanović O, Vujanić D, Dobrijević M, Trbojević I, Sladojević Ž, Laušević D, Nedić D. Severe form of generalized sarcoptic mange with secondary piotraumatic folliculitis in dogs: differential diagnosis and epizootiological approach. Ветеринарски Журнал Републике Српске 2020; 201-2.

Uzuegbu M. Oluchi Sarcoptic mange in a dog: A case study Merit Research. IJBBB 2015; 3:005-008.

Downloads

Published

2023-02-06

How to Cite

Emre Yanar, K., Kucukler, S., Eren, E., Sertaç Eroglu, M., Ilgun, M., Gur, C., Kandemir, F. M., & Aktas, M. S. (2023). Oxidative stress parameters in dogs naturally infected with sarcoptic mange. Revista Colombiana De Ciencias Pecuarias, 36(4), 172–180. https://doi.org/10.17533/udea.rccp.v36n4a2

Issue

Section

Accepted manuscripts

Similar Articles

> >> 

You may also start an advanced similarity search for this article.