Effects of dietary supplementation with Erythrina americana on seminal characteristics of rams
DOI:
https://doi.org/10.17533/udea.rccp.v37n4a5Keywords:
artificial insemination, antioxidants, Erythrina americana, fresh semen, rams, refrigerated semen, seminal quality, sheep, spermatozoaAbstract
Background: Antioxidants of natural origin can be used to improve cryopreservation of semen. Objective: To evaluate the effect of dietary supplementation with Erythrina americana on the seminal characteristics of rams. Methods: Ten rams were randomly distributed in two treatments: l) Group supplemented with E. americana (20% dry matter basis; AEA, n=5), and 2) Control group not supplemented (SEA, n=5). Both diets were iso-proteic and iso-energetic. The feeding period was eleven weeks. Ejaculates were obtained through an artificial vagina and were evaluated fresh (37 °C) and refrigerated (5 °C). Semen volume (VOL) was assessed by visual examination in a calibrated (mm) tube; sperm concentration (CON x106) with a photometer; and mass motility (MM, 1-5), individual motility (IM), normality (N), and live sperm (L) were assessed by microscopic observation. Results: Feeding E. americana did not affect (p>0.05) VOL, CON, MM, and N in fresh semen, but decreased (p<0.05) IM and L. The L and N variables did not differ (p>0.05) among treatment groups in the refrigerated semen; however, IM improved in the AEA group (p<0.05) compared to the SEA group. Conclusion: Dietary supplementation of rams with E. americana reduces sperm quality in fresh semen but is beneficial for the cooling process of refrigerated semen.
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Abadjieva D, Yotov S, Mladenova V, Lauberte L, Kalvanov I, Krasilnikova J, Telesheva G, Kistanova E. Positive effect of natural antioxidant oregonin from Alnus incana bark on ram semen quality stored at 5 °C for 48 h. Res Vet Sci 2020; 131:153–158. https://doi.org/10.1016/j.rvsc.2020.04.021
Abdi-Benemar H, Khalili B, Zamiri MJ, Ezazi H, Sotoudeh, AG, Hamidzadeh S, Simanoorf N. Effects of astaxanthin supplementation on the freezability, lipid peroxidation, antioxidant enzyme activities and post-thawing fertility of ram semen. Small Rum Res 2020; 192:106213. https://doi.org/10.1016/j.smallrumres.2020.106213
Allai L, Benmoula A, Maia MD, Nasser B, Amiri BE. Supplementation of ram semen extender to improve seminal quality and fertility rate. Anim Reprod Sci 2018; 192:6–17. https://doi.org/10.1016/j.anireprosci.2018.03.019
Al-Najar EM, Abdullah AM, Al-Rubaye TA, Hadi, SM. Evaluation of Marjoram Leaves (Oregano vulgar) as Feed Supplement on Quality of Semen in Awasian Pollination Rams. Arch Razi Inst 2022; 77(5):1831–1835. https://doi.org/10.22092/ari.2022.358366.2211
Association of Official Analytical Chemists (AOAC). Official methods of analysis Association Official Analytical Chemists. 20th Edition. Arlington, VA. 2017.
Attia Y, Hamed R, Bovera F. Semen quality, antioxidant status and reproductive performance of rabbit’s bucks fed milk thistle seeds and rosemary leaves. Anim Repro Scie 2017; 184:178–186. https://doi.org/10.1016/j.anireprosci.2017.07.014
Baghshahi H, Riasi A, Mahdavi AH, Shirazi A. Antioxidant effects of clove bud (Syzygium aromaticum) extract used with different extenders on ram spermatozoa during cryopreservation. Cryobiology 2014; 69:482–487. https://doi.org/10.1016/j.cryobiol.2014.10.009
Bucak MN, Ateşşahin A, Yüce A. Effect of anti-oxidants and oxidative stress parameters on ram semen after the freeze–thawing process. Small Rumin Res 2008; 75:128–134. https://doi.org/10.1016/j.smallrumres.2007.09.002
Cai L, Li YP, Wei ZX, Li XL, Jian XR. Effects of dietary gallic acid on growth performance, diarrhea incidence, intestinal morphology, plasma antioxidant indices, and immune response in weaned piglets. Anim Feed Sci Tec 2020; 261:114391. https://doi.org/10.1016/j.anifeedsci.2020.114391
Câmara DR, Mello-Pinto MMC, Pinto LC, Brasil OO, Nunes JF, Guerra MMP. Effects of reduced glutathione and catalase on the kinematics and membrane functionality of sperm during liquid storage of ram semen. Small Rumin Res 2011; 100:44–49. https://doi.org/10.1016/j.smallrumres.2011.05.010
CIOMS (Council for international Organizations of Medical Sciences). “International Guiding Principles for Biomedical Research Involving Animals”. 2016. Geneva, Switzerland.
Córdova IA, Lang CR, Córdova C, Córdova M, Guerra J, Rodríguez B, Arancibia K. Estrés oxidativo y antioxidantes en la conservación espermática. Rev Comp Cien Vet 2009; 3(1):01–38.
Davendra K, Krishnappa B. Recent advances in cryopreservation of semen and insemination in sheep: A review. Indian Journal of Small Ruminants 2019; 25(2):134–137. https://doi.org/10.5958/0973-9718.2019.00043.6
Del Valle I, Souter A, Maxwell WM, Muiño-Blanco T, Cebrián-Pérez JA. Function of ram spermatozoa frozen in diluents supplemented with casein and vegetable oils. Anim Rep Sci 2013; 138:213–219. https://doi.org/10.1016/j.anireprosci.2013.02.022
DOF (Diario Oficial de la Federación). “Norma Oficial Mexicana NOM-062-ZOO-1999: Especificaciones técnicas para la producción, cuidado y uso de animales de laboratorio”. 2001. México, D.F.
Embuscado ME. Spices and herbs: Natural sources of antioxidants–a mini review. J Fun Foods 2015; 18:811–819. https://doi.org/10.1016/j.jff.2015.03.005
Ezazi H, Abdi-Benemar H, Taghizadeh A, Khalili B, Seifdavati J, Jafaroghli M, Elghandour M, Salem AZ. The influence of dietary sunflower oil, rich in n-6 polyunsaturated fatty acids, in combination with vitamin C on ram semen parameters, sperm lipids and fertility. J Sci Food Agric 2019. 99(8): 3803–3810. https://doi.org/10.1002/jsfa.9602
Forouzanfar M, Sharafi M, Hosseini SM, Ostadhosseini S, Hajian M, Hosseini L, Abedi P, Nili N, Rahmani HR, Nasr-Esfahani MH. In vitro comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of ram semen. Theriogenology 2010; 73:480–487. https://doi.org/10.1016/j.theriogenology.2009.10.005
García, E. Modificaciones al Sistema de Clasificación Climática de Köppen (para adaptarlo a las condiciones de la República Mexicana) 5° ed. México: Instituto de Geografía, Universidad Nacional Autónoma de México (UNAM); 2004. p. 50–56.
García-Mateos R, Soto-Hernández M, Vibrans H. Erythrina americana Miller “Colorín”: Fabaceae, a versatile resource from Mexico: A Review. Economic Botany 2001; 55(3):391–400. https://doi.org/10.1007/BF02866562
Ibarra EE, Pacheco SM, García MR, San Miguel CR, Ramírez VG, Soto HR. Actividad antioxidante de alcaloides de Erythrina americana Miller. Rev Fit Mex 2011; 34(4):241–246. https://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0187-73802011000400005
Kaeoket K, Sang-urai P, Thamniyom A, Chanapiwat P, Techakumphu, M. Effect of Docosahexaenoic Acid on Quality of Cryopreserved Boar Semen in Different Breeds. Reprod Dom Anim 2010; 45:458–463. https://doi.org/10.1111/j.1439-0531.2008.01239.x
Kasimanickam R, Pelzer KD, Kasimanickam V, Swecker WS, Thatcher CD. Association of classical semen parameters, sperm DNA fragmentation index, lipid peroxidation and antioxidant enzymatic activity of semen in ram-lambs. Theriogenology 2006; 65:1407–1421. https://doi.org/10.1016/j.theriogenology.2005.05.056
Loizzo MR, y Tundis R. Plant Antioxidant for Application in Food and Nutraceutical Industries. Antioxidants (Basel, Switzerland) 2019; 8(10):453. https://doi.org/10.3390/antiox8100453
Liu Q, Duan RJ, Zhou YF, Wei HK, Peng J, Li JL. Supplementing oregano essential oil to boar diet with strengthened fish oil: Effects on semen antioxidant status and semen quality parameters. Andrologia 2016; 49(10):1–8. https://doi.org/10.1111/and.12764
Marti E, Marti JI, Muiño-Blanco T, Cebrián-Pérez JA. Effect of the cryopreservation process on the activity and immunolocalization of antioxidant enzymes in ram spermatozoa. J. Androl 2008; 29:459–467. https://doi.10.2164/jandrol.107.003459
Mata-Campuzano M, Álvarez-Rodríguez M, Tamayo-Canul J, López-Urueña E, de Paz P, Anel L, Martínez-Pastor F, Álvarez M. Refrigerated storage of ram sperm in presence of Trolox and GSH antioxidants: Effect of temperature, extender and storage time. Anim Reprod Sci 2014; 151:137–147. https://doi.org/10.1016/j.anireprosci.2014.10.006
Mata-Campuzano M, Soleilhavoup C, Tsikis G, Martinez-Pastor F, de Graaf SP, Druart X. Motility of liquid stored ram spermatozoa is altered by dilution rate independent of seminal plasma concentration. Anim Reprod Sci 2015; 162:31–36. https://doi: 10.1016/j.anireprosci.2015.09.004
Motlagh MK, Sharafi M, Zhandi M, Mohammadi-Sangcheshmeh A, Shakeri M, Soleimani M, Zeinoaldini S. Antioxidant effect of rosemary (Rosmarinus officinalis L.) extract in soybean lecithin-based semen extender following freeze-thawing process of ram sperm. Cryobiology 2014; 69:217–222. https://doi.org/10.1016/j.cryobiol.2014.07.007
Pino RS, Prieto G, Pérez M, Molina, TJ. Género Erythrina: Fuente de metabolitos secundarios con actividad biológica. Acta Farmacéutica Bonaerense 2004; 23(2):252–258.
Purdy PH, Stobart EM, Murdoch WJ, Moss GE, Larson B, Ramsey S, Graham JK, Blackburn HD. The fertility of ram sperm held for 24h at 5°C prior to cryopreservation. Anim Repro Sci 2010; 118(2):231–235. https://doi.org/10.1016/j.anireprosci.2009.06.014.
Quintero EJ, Sánchez FC, Olguín AH. Contribución en el desarrollo de un índice de calidad del semen para la valoración de sementales ovinos. Veterinaria 2016; 58(1):105–109. https://erevistas.uacj.mx/ojs/index.php/culcyt/article/view/1395
Salamon S, Maxwell WMC. Storage of ram semen. Anim Reprod Sci 2000; 62:77–111. https://doi.org/10.1016/S0378-4320(00)00155-X
Rehman F, Zhao C, Shah MA, Qureshi MS, Wang X. Semen extenders and artificial insemination in Ruminants. Veterinaria 2013; 1:1–8.
Russel AJF, Doney JM, Gunn RG. Subjective assessment of fat in live sheep. J Agricult Sci 1969; 72: 451–454.
Sapanidou V, Taitzoglou I, Tsakmakidis I, Kourtzelis I, Fletouris D, Theodoridis A, Lavrentiadou S, Tsantarliotou M. Protective effect of crocetin on bovine spermatozoa against oxidative stress during in vitro fertilization. Andrology 2016; 4:1138–1149. https://doi.org/10.1111/andr.12248
Statistical Analysis System Institute (SAS). SAS User’s Guide: Statistics (Version 5). Cary, N.C. USA. 2002. Inst. Inc. 584 pp.
Vahedi V, Hedayat EN, Behroozlak M, Dirandeh E. Antioxidant effects of thyme (Thymus vulgaris) extract on ram sperm quality during cryopreservation. Iran Jour Appl Anim Sci 2018; 8(2):263–269.https://ijas.rasht.iau.ir/article_540373.html
Vizzari F, Massányi M, Knízatová N, Corino C, Rossi R, Ondruška L, Tirpák F, Halo M, Massányi P. Effects of dietary plant polyphenols and seaweed extract mixture on male-rabbit semen: Quality traits and antioxidant markers. Saudi Jour Biol Sci 2021; 28(1):1017–1025. https://doi.org/10.1016/j.sjbs.2020.11.043
Zhao J, Jin Y, Du M, Liu W, Ren Y, Zhang C, Zhang J. The effect of dietary grape pomace supplementation on epididymal sperm quality and testicular antioxidant ability in ram lambs, Theriogenology 2017; 97:50–56. https://doi.org/10.1016/j.theriogenology.2017.04.010
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