Coffee pulp supplementation prior to breeding improves oxidative status without affecting fertility of primiparous ewes

Keywords: antioxidant capacity, caffeine, coffee pulp, estrus synchronization, ewes, fertility lipoperoxidation, oxidative stress, reproductive parameters, sheep


Background: Coffee pulp has a high content of antioxidants capable of modifying the oxidative status in small ruminants. However, high amounts for a prolonged time can reduce fertility. Objective: To determine the effect of two inclusion levels of coffee pulp during estrous synchronization on reproductive variables and oxidative status of primiparous ewes. Methods: Sixty Suffolk x Dorset primiparous ewes were distributed into three treatments in a completely randomized design; T0: (n=20) 1.5 kg balanced diet, Tl: (n=20) 1.5 kg balanced diet and 5% coffee pulp, T2: (n=20) 1.5 kg balanced diet and 10% coffee pulp. Supplementation was given for 16 days before estrus synchronization and until the beginning of the breeding season. A progestogen (CIDR®) was inserted for ll days and a dose of PGF2a was applied two days prior to its withdrawal. Estrus detection started 12 hours after CIDR withdrawal. Blood samples were obtained during the supplementation period to measure oxidative status, antioxidant capacity, glucose and insulin, and up to 9 days after breeding to determine progesterone concentration. Pregnancy diagnosis was performed at 30 and 60 days post-breeding. An analysis of repeated measures of mixed effects and frequency analysis were carried out. Results: Inclusion of coffee pulp for a short period prior to breeding did not affect reproductive parameters, nor progesterone, glucose or insulin concentrations (p>0.05); however, antioxidant capacity increased, while lipid oxidation showed an opposite trend (p<0.05). Conclusion: Inclusion of up to 10% coffee pulp in the diet of ewe lambs for 16 days prior to breeding improves oxidative status without causing adverse effects on pregnancy, estrus or prolificacy.

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Author Biographies

Lizbet K. Gutiérrez-Prado, Colegio de Postgraduados

Livestock Program, Colegio de Postgraduados, Montecillo Campus, Texcoco, State of México, México.

María T. Sánchez-Torres-Esqueda, Colegio de Postgraduados

Livestock Program, Colegio de Postgraduados, Montecillo Campus, Texcoco, State of México, México.

Teodulo Salinas-Ríos, Benito Juárez Autonomous University of Oaxaca

Faculty of Veterinary Medicine and Zootechnics, Benito Juárez Autonomous University of Oaxaca, Oaxaca, México.

José L. Figueroa-Velasco, Colegio de Postgraduados

Livestock Program, Colegio de Postgraduados, Montecillo Campus, Texcoco, State of México, México.

José A. Martínez-Aispuro, Colegio de Postgraduados

Livestock Program, Colegio de Postgraduados, Montecillo Campus, Texcoco, State of México, México.

David A. Contreras-Caro-Del-Castillo, National Autonomous University of México

Department of Veterinary Medicine and Ruminant Zootechnics (FMVZ), National Autonomous University of México (UNAM), México City, México.

Mario Cárdenas-León, Salvador Zubirán National Institute of Medical Sciences and Nutrition
Laboratorio de Biología de la Reproducción, Salvador Zubirán National Institute of Medical Sciences and Nutrition, Tlalpan, Ciudad de México, México.  
Cuauhtémoc Nava-Cuellar, National Autonomous University of México

Department of Biochemistry, (FMV), National Autonomous University of México, México City, México.

José L. Cordero-Mora, Colegio de Postgraduados

Livestock Program, Colegio de Postgraduados, Montecillo Campus, Texcoco, State of México, México.

José L. García-Cue, Colegio de Postgraduados

Livestock Program, Colegio de Postgraduados, Montecillo Campus. Statistical and Informatics Socioeconomics Program, Colegio de Postgraduados, Montecillo Campus, Texcoco, State of México.


Arellano GM, Ramírez CA, Mancera TT, Pérez MG, Saucedo CG. Antioxidant activity of fermented and on-fermented coffee (Coffea arabica) pulp extracts. Food Technol Biotechnol 2011; 49:374–378.

Agarwal A, Gupta S, Sharma RK. Role of oxidative stress in female reproduction. Reprod Biol Endocrinol 2005; 14:3-28. https: 10.1186/1477-7827-3-28

Balaro MF, Brandão FZ, Peneiras AB, Oba E, da Fonseca JF, Almosny NR, da Cruz Cardoso E. Reproductive performance, metabolic and hormonal profiles of Santa Inês ewes in winter and summer under tropical conditions. Trop Anim Health Prod 2015; 47(3):627-631. https: 10.1007/s11250-015-0757-z

Benowitz NL. Clinical pharmacology of caffeine. Annu Rev Med 1990; 41(1):277-288. https: 10.1146/

Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Anal Biochem 1996; 239:70–76. https: 10.1006/abio.1996.0292

Berchieri-Ronchi CB, Kim SW, Kim SW, Zhao Y, Correa CR, Yeum KJ, Ferreira AL. Oxidative stress status of highly prolific sows during gestation and lactation. Animal 2011; 5(11):1774-1779. https: 10.1017/S1751731111000772.

Bilge U, nlüoğlu İ, Gültekin GS, Özgül MN, ŞuleYN, Bilgin M. The acute effect of coffee intake on hormones that affect glucose and glucose metabolism in healthy individuals. Biomed Res 2017; Special Issue: S531-S536.

Casamassima D, Palazzo M, Martemucci G, Vazzarri F, Corino C. Effects of verbascoside on plasma oxidative status and blood and milk production parameters during the peripartum period in Lacaune ewes. Small Rumin Res 2012; 105:1-8. https:10.1016/j.smallrumres.2011.12.001

Castillo C, Hernández J, Bravo A, Lopez-Alonso M, Pereria V, Benedito JL. Oxidative status during late pregnancy and early lactation in dairy cows. Vet J 2005; 169:286–292. https: 10.1016/j.tvjl.2004.02.001

Catunda AGV, Lima ICS, Bandeira GC, Gadelha, Pereira ES, Salmito-Vanderley CSB, Araújo AA, GA Martins, Campos CAN. Blood leptin, insulin and glucose concentrations in hair sheep raised in a tropical climate. Small Rumin Res 2013; 114:272–279. https: 10.1016/j.smallrumres.2013.07.008

Daly JW. Mechanism of action of caffeine. Caffeine, Coffee, and Health, Raven Press, New York 1993; pp. 97–150.Diskin MG, Morris DG. Embryonic and early foetal losses in cattle and other ruminants. Reprod Domest Anim 2008; 43:260–267. https: 10.1111/j.1439-0531.2008.01171.x

Du Y, Melchert HU, Knopf H, Braemer-Hauth, Pabel E. Association of serum caffeine concentrations with serum glucose levels in caffeine‐drug users and non‐users – results of German National Health Surveys. Diabetes, Obes Metab 2006; 9:756–758. https: 10.1111/j.1463-1326.2006.00689.x

Echeverria MC, Nuti M. Valorisation of the residues of coffee agro-industry: perspectives and limitations. Open Waste Manag J 2017; 10:13-22. https: 10.2174/1876400201710010013

Flores O, Velázquez R, López R, Benítez S, Oropeza A, Hernández C. Porcentaje de concepción en vacas lecheras tratadas con progesterona cinco días después de la inseminación. Rev Mex Cienc Pecu 2013; 4(4):507-514

Fumihiro S, Hideto Y, Kana S, Yasuaki S, Emi H, Mamoru M. Caffeine intake, CYP1A2 polymorphism and the risk of recurrent pregnancy loss. Mol Hum Reprod 2005; 11:357-60. https: 10.1093/molehr/gah175

Guerin P, El Mouatassim S, Ménezo Y. Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings. Hum Reprod Update 2001; 7(2):175-189. https: 10.1093/humupd/7.2.175

Gupta S, Agarwal A, Banerjee J, Alvarez J. The role of oxidative stress in spontaneous abortion and recurrent pregnancy loss: a systematic review. Obstet Gynecol Surv 2007; 62(5):335–347. https: 10.1097/01.ogx.0000261644.89300.df

Herrera E. Metabolismo lipídico durante la gestación. Alim Nut Salud 1997; 4(2):43-49.Kaneko JJ. Clinial Biochemistry of Domestic Animals. Third edition; 1980.

Kankofer M, Albera E, Feldman M, Gundling N, Hoedemaker M. Comparison of antioxidative/oxidative profiles in blood plasma of cows with and without retained fetal placental membranes. Theriogenology 2010; 74:1385–1395. https: 10.1016/j.theriogenology.2010.06.009

Lima de Souza A, García R, Cabral LS, Albuquerque PM, Ferreira DV. Coffee hull in the diet of dairy heifers: nitrogen balance and microbial protein synthesis. Rev Bras Zootec 2010; 39(5):1141-1145. https: 10.1590/S1516-35982010000500027

Londoño L, Naranjo C, Quintero O, Mónica M. Estudio de los cambios de la actividad antioxidante en bebidas de café durante su periodo de vida útil usando métodos in-vitro y ex− vivo. Vitae 2013; 20(2):95-104.

Mahmoud S, Azab M. Regulation of glucose level durng late pregnancy and onset of lactation in Egyptian female Baladi goats. Small Rumin Res 2014; 121:320-324. https: 10.1016/j.smallrumres.2014.06.0091

Molina SFP, I Alapont VM. Restringir la ingesta de cafeína en embarazadas a partir de las 20 semanas de embarazo no tiene impacto en el peso de nacimiento del neonato ni en la duración de la gestación. Evid Pediatr 2007; 3(2):45. https: vol3/2007_numero_2/2007_vol3_numero2.16.htm

Murthy PS, Naidu MM. Recovery of Phenolic Antioxidants and functional compounds from coffee industry By-Products. Food Bioproc Tech 2012; 5:897–903. https: 10.1007/s11947-010-0363-z

Natarajan G, Lulic-Botica M, Aranda JV. Pharmacology Review: Clinical Pharmacology of caffeine in the newborn. Neo Rev 2007; 5:214-221. https: 10.1542/neo.8-5-e214

Nawrot P, Jordan S, Eastwood J. Effects of caffeine on human health. Food Addit Contam 2003; 20:1-30. https: 10.1080/0265203021000007840

Nieto G, Díaz P, Bañon S, Garrido MD. Dietary administration of ewe diets with a distillate from rosemary leaves (Rosmarinus officinalis L.): Influence on lamb meat quality. Meat Sci 2010; 84:23-29. https: 10.1016/j.meatsci.2009.08.001

Noro M, Vargas V, Pulido RG, Wittwer F. Efecto del tipo de concentrado sobre indicadores sanguíneos del metabolismo de energía y de proteínas en vacas lecheras en pastoreo primaveral. Arch Med Vet 2006; 38(3):227-232. https: org/10.4067/S0301-732X2006000300005

NRC. Nutrient Requirements of Sheep, Sixth Revised. Washington, D.C; 1985.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem1979; 95:351-358. https: 10.1016/0003-2697(79)90738-3

Pedraza-Beltrán P, Estrada-Flores JG, Martínez-Campos AR, Estrada-López I, Rayas-Amor AA, Yong-Angel G, Figueroa-Medina M, Avilés Nova F, Castelán-Ortega OA. On-farm evaluation of the effect of coffee pulp supplementation on milk yield and dry matter intake of dairy cows grazing tropical grasses in central Mexico. Trop Anim Health Prod 2012; 44(2):329–336. https: 10.1007/s11250-011-0025-9

Pérez-Hernández L, Chávez-Quiroz K, Medina-Juárez L, Gámez-Meza N. Compuestos fenólicos, melanoidinas y actividad antioxidante de café verde y procesado de las especies Coffea arabica y Coffea canephora. Biotecnia 2013; 15(1):51-56. https: 10.18633/bt.v15i1.136

Razz R, Clavero T. Niveles de urea, fósforo, glucosa e insulina de vacas en ordeño suplementadas con concentrado en un sistema de Panicum maximum y Leucaena leucocephala. REV CIENT-FAC CIEN V 2004; 14(4):365-369.

Restrepo-Sánchez DC, Narváez-Cuenca CE, Restrepo-Sánchez LP. Extracción de compuestos con actividad antioxidante de frutos de guayaba cultivada en Vélez-Santander, Colombia. Quím Nova 2009; 32:1517-1522. https: 10.1590/S0100-40422009000600030

SAGARPA, Secretaría de Agricultura, Ganadería, Desarrollo Rural, Pesca y Alimentación. Norma Oficial Mexicana NOM-062-ZOO-1999, especificaciones técnicas para la producción, cuidado y uso de los animales de laboratorio. Diario Oficial de la Federación 2001. 75:113-160.

Salinas-Rios T, Ortega-Cerrilla ME, Sánchez-Torres-Esqueda MT, Hernández-Bautista J, Díaz-Cruz A, Figueroa-Velasco JL, Guinzberg-Perrusquía R, Cordero-Mora JL. Productive performance and oxidative status of sheep fed diets with coffee pulp. Small Rum Res 2015; 123(1):17-21. https: 10.1016/j.smallrumres.2014.09.008

Salinas-Rios T, Sánchez-Torres-Esqueda MT, Díaz-Cruz A, Cordero-Mora JL, León MC, Hernández-Bautista J, Aquino RN. Oxidative status and fertility of ewes supplemented coffee pulp during estrous synchronization and early pregnancy. Rev Colomb Cienc Pec 2016; 29(4):255-263. https: 10.17533/udea.rccp.v29n4a02

Salinas-Rios T, Sánchez-Torres-Esqueda MT, Ortega-Cerilla ME, Soto-Hernández M, Díaz-Cruz A, Hernández-Bautista J, Nava-Cuellar C, Vaquera-Huerta H. Changes in composition, antioxidant content, and antioxidant capacity of coffee pulp during the ensiling process. Rev Bras Zootec 2014; 43(9):492-498. https: 10.1590/S1516-35982014000900006

SAS®, Statistical Analisys System. SAS/STAT User ́s Guide. Proceeding Versión 9. SAS Institute Incorporation. Cary, NC, USA; 2002.

Sönmez M, Bozkurt T, Türk G, Gür S, Kizil M, Yüce A. The effect of vitamin E treatment during preovulatory period on reproductive performance of goats following estrous synchronization using intravaginal sponges. Anim Reprod Sci 2009; 114:183-192. https: 10.1016/j.anireprosci.2008.09.007

Spears JW, Weiss WP. Role of antioxidants and trace elements in healt and immunity of transition dairy cows. Vet J 2008; 176:70-76.10.1016/j.tvjl.2007.12.015

Valenzuela A. El café y sus efectos en la salud cardiovascular y en la salud materna. Rev Chil Nutr 2010; 37(4):514-523. https: 10.4067/S0717-75182010000400013

Van Dam RM, Pasman WJ, Verhoef P. 2004. Effects of Coffee Consumption on Fasting Blood Glucose and Insulin Concentrations:randomized controlled trials in healthy volunteers. Diabetes Care 2004; 27(12):2990-2992. https: 10.2337/diacare.27.12.2990

Wesselink AK, Wise LA, Rothman KJ, Hahn KA, Mikkelsen EM, Mahalingaiah y Hatch EE. Caffeine and caffeinated beverage consumption and fecundability in apreconception cohort. Reprod Toxicol 2016; 62:39–45. https: 10.1016/j.reprotox.2016.04.022

Zhang W, Zhang KY, Ding XM, Bai SP, Hernandez JM, Yao B, Zhu Q. Influence of canthaxanthin on broiler breeder reproduction, chick quality, and performance. Poult Sci 2011; 90(7):1516-1522. https: 10.3382/ps.2010-01126

How to Cite
Gutiérrez-Prado L. K., Sánchez-Torres-Esqueda M. T., Salinas-Ríos T., Figueroa-Velasco J. L., Martínez-Aispuro J. A., Contreras-Caro-Del-Castillo D. A., Cárdenas-León M., Nava-Cuellar C., Cordero-Mora J. L., & García-Cue J. L. (2019). Coffee pulp supplementation prior to breeding improves oxidative status without affecting fertility of primiparous ewes. Revista Colombiana De Ciencias Pecuarias, 32(4), 261-273.
Original research articles