Cryopreservation method and composition of the vitrification solution affect viability of in vitro bovine embryos

José N Vargas Reyes; Liliana Chacón Jaramillo

doi:10.17533/udea.rccp.324972

Autores/as

José N Vargas Reyes Universidad de la Salle
Liliana Chacón Jaramillo Universidad de la Salle

DOI:

https://doi.org/10.17533/udea.rccp.324972

Palabras clave:

congelación lenta, eclosión, etilenglicol, expansión

Resumen

no existe una formula específica ideal para la congelación de embriones bovinos producidos in vitro (IVP). Objetivo: estimar si la composición de diferentes soluciones de vitrificación afecta la viabilidad de embriones bovinos IVP en comparación con embriones criopreservados mediante el método convencional de congelación lenta. Métodos: los embriones bovinos producidos in vitro se congelaron a través de los siguientes métodos: 1) congelación lenta (1,5 M de etilenglicol (EG)), 2) por vitrificación utilizando dos soluciones vitrificantes: (1) Protocolo V1: solución vitrificante comercial, (2) Protocolo V2: solución vitrificante (20% EG, 20% dimetil sulfóxido (DMSO), 20% suero fetal bovino (FBS) y de calentamiento (20% FBS, 0,2 M sucrosa). La viabilidad embrionaria se evaluó a las 24, 48 y 72 h post-descongelación, a través de la medición del porcentaje de embriones que se expandieron y llegaron al estadio de eclosión. Resultados: el porcentaje de expansión embrionaria a 24 h fue más alto en los embriones que fueron vitrificados con los protocolos V1 y V2 (89,0%, 86,2%, respectivamente), que aquellos que fueron criopreservados con el método de congelación lenta (73,6%, p < 0,05). Igualmente, un porcentaje mayor de embriones criopreservados por el método de vitrificación eclosionó a las 72 h, resultando en un mayor porcentaje en los embriones vitrificados con el protocolo V2 (84,3%), en comparación con el protocolo V1 (64,0%, p < 0,05), y ambos porcentajes fueron más altos que los obtenidos con la congelación lenta (55,2%, p < 0,05). Conclusiones: el método de congelación y la composición de la solución de vitrificación afectan la viabilidad post-congelación de los embriones bovinos IVP.

|Resumen

= 568 veces | PDF (ENGLISH)

= 155 veces| | HTML (ENGLISH)

= 24 veces|

Descargas

Los datos de descargas todavía no están disponibles.

Citas

Abdalla H, Shimoda M, Hara H, Morita H, Kuwayama M, Hirabayashi M, Hochi S: Vitrification of ICSI-and IVF-derived bovine blastocysts by minimum volume cooling procedure: effect of developmental stage and age. Theriogenology 2010; 74:1028-1035.Caamaño J, Gómez E, Trigal B, Muñoz M, Carrocera S, Martín D, Díez C. Survival of vitrified in vitro–produced bovine embryos after a one-step warming in-straw cryoprotectant dilution procedure. Theriogenology 2015; 83(5):881-890.George F, Vrancken M, Verhaeghe B, Verhoeye F, Schneider Y-J, Massip A, Donnay I. Freezing of in vitro produced bovine embryos in animal protein-free medium containing vegetal peptones. Theriogenology 2006; 66:1381-1390.

Ha AN, Park HS, Jin JI, Lee SH, Ko DH, Lee DS, Kong IK. Postthaw survival of in vitro-produced bovine blastocysts loaded onto the inner surface of a plastic vitrification straw. Theriogenology 2014; 81:467-473.Hasler JF. The current status and future of commercial embryo transfer in cattle. Anim Reprod Scie 2003; 79:245-264.Holm P, Booth PJ, Schmidt MH, Greve T, Callesen H. High bovine blastocyst development in a static in vitro production system using SOFaa medium supplemented with sodium citrate and myo-inositol with or without serum-proteins. Theriogenology 1999; 52:683-700.Imai K, Kobayashi S, Goto Y, Dochi O, Shimohira I. Cryopreservation of bovine embryos obtained by in-vitro culture of IVM-IVF oocytes in the presence of linoleic acid albumin. Theriogenology 1997; 47:347-347.Inaba Y, Aikawa Y, Hirai T, Hashiyada Y, Yamanouchi T, Misumi K, Ohtake M, Somfai T, Kobayashi S, Saito N, Matoba S, konishi K, Imai K. In-straw cryoprotectant dilution for bovine embryos vitrified using cryotop. J Reprod Dev 2011; 57:437-443.Kaidi S, Donnay I, Van Langendonckt A, Dessy F, Massip A. Comparison of two co-culture systems to assess the survival of in vitro produced bovine blastocysts after vitrification. Anim Reprod Sci 1998; 52:39-50.Kuwayama M. Highly efficient vitrification for cryopreservation of human oocytes and embryos: the Cryotop method. Theriogenology 2007; 67:73-80.Lim KT, Jang G, Ko K H, Lee WW, Park HJ, Kim JJ, Lee BC. Improved cryopreservation of bovine preimplantation embryos cultured in chemically defined medium. Anim Reprod Sci 2008; 103:239-248.Mucci N, Aller J, Kaiser GG, Hozbor F, Cabodevila J, Alberio RH. Effect of estrous cow serum during bovine embryo culture on blastocyst development and cryotolerance after slow freezing or vitrification. Theriogenology 2006; 65:1551-1562.Naik BR, Rao BS, Vagdevi R, Gnanprakash M, Amarnath D, Rao V. Conventional slow freezing, vitrification and open pulled straw (OPS) vitrification of rabbit embryos. Anim Reprod Sci 2005; 86:329-338.Nedembale TL, Dinnyés A, Groen W, Dobrinsky J R, Tian XC, Yang X. Comparison on in vitro fertilized bovine embryos cultured in KSOM or SOF and cryopreserved by slow freezing or vitrification. Theriogenology 2004; 62:437-449.Perry G. 2012 statistics of embryo collection and transfer in domestic farm animals. Data retrieval Committee reports, International Embryo transfer Society, IETS December 2013. Pollard JW, Leibo SP. Chilling sensitivity of mammalian embryos. Theriogenology 1994; 41:101-106.Rios GL, Mucci NC, Kaiser GG, Alberio RH. Effect of container, vitrification volume and warming solution on cryosurvival of in vitro-produced bovine embryos. Anim Reprod Sci 2010; 118:19-24. Rizos D, Gutierrez-Adan A, Perez-Garnelo S, De La Fuente J, Boland MP, Lonergan P. Bovine embryo culture in the presence or absence of serum: implications for blastocyst development, cryotolerance, and messenger RNA expression. Biol Reprod 2003; 68:236-243.Saragusty J, Arav A. Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification. Reproduction 2011, 141(1):1-19.Schmidt M, Greve T, Avery B, Beckers JF, Sulon J, and Hansen HB. Pregnancies, calves and calf viability after transfer of in vitro produced bovine embryos. Theriogenology 1996; 46:527-539.Shirazi A, Soleimani M, Karimi M, Nazari H, Ahmadi E, Heidari B: Vitrification of in vitro produced ovine embryos at various developmental stages using two methods. Cryobiology 2010, 60:204-210.Statistix 8, 2003. Statistix8: Analytical software user’s manual. Tallahassee, Florida.Stinshoff H, Wilkening S, Hanstedt A, Brüning K, Wrenzycki C: Cryopreservation affects the quality of in vitro produced bovine embryos at the molecular level. Theriogenology 2011, 76:1433-1441.Trigal B, Gómez E, Caamaño JN, Muñoz M, Moreno J, Carrocera S, Martin D, Diez C. In vitro and in vivo quality of bovine embryos in vitro produced with sex-sorted sperm. Theriogenology 2012; 78:1465-1475.Vajta G. Holm P, Kuwayama M, Booth PJ, Jacobsen H, Greve T, Callesen H. Open pulled straw (OPS) vitrification: a new way to reduce cryoinjuries of bovine ova and embryos. Mol Reprod Dev 1998; 5:53-58.Vajta G. Vitrification of the oocytes and embryos of domestic animals. Anim Reprod Sci 2000; 60:357-364.Vajta G, Nagy ZP. Are programmable freezers still needed in the embryo laboratory? Review on vitrification. Reprod BioMed Online 2006; 12:779-796.Vajta G. Vitrification in human and domestic animal embryology: work in progress. Reprod Fert Dev 2013; 25:719-727.Wrenzycki C, Herrmann D, Niemann H. Messenger RNA in oocytes and embryos in relation to embryo viability. Theriogenology 2007; 68:S77-S83.Xu J, Guo Z, Su L, Nedambale T, Zhang J, Schenk J, Moreno J, Dinnyés A, Ji W, Tian X. Developmental potential of vitrified Holstein cattle embryos fertilized in vitro with sex-sorted sperm. J Dairy Sci 2006; 89:2510-2518.Zhao Xm, Du Wh, Wang D, Hao Hs, Qin T, Liu Y, Zhu Hb. Controlled freezing and open-pulled straw (OPS) Vitrification of in vitro produced bovine blastocysts following analysis of ATP content and reactive oxygen species (ROS) level. J Integr Agr 2012; 11:446-455.