Effect of increasing levels of Chlorella spp. on the in vitro fermentation and methane production of a corn silage-based diet

Juan de J Vargas; Federico Tarnonsky; Araceli Maderal; Ignacio Fernández-Marenchino; Federico Podversich; Tessa M Schulmeister; Nicolás DiLorenzo

doi:10.17533/udea.rccp.v37n1a2

Autores/as

Juan de J Vargas University of Florida
Federico Tarnonsky University of Florida
Araceli Maderal University of Florida
Ignacio Fernández-Marenchino University of Florida
Federico Podversich University of Florida
Tessa M Schulmeister University of Florida
Nicolás DiLorenzo University of Florida

DOI:

https://doi.org/10.17533/udea.rccp.v37n1a2

Palabras clave:

aditivos, dietas bajas en proteína, forrajes conservados, metanogénesis, microalgas verdes, sistemas de cría, sistemas de levante, suplementación proteica

Resumen

Antecedentes: Generalmente, los forrajes en los sistemas de producción de cría y levante de ganado tienen baja cantidad de proteína cruda y alta de fibra, limitando la productividad animal e incrementando la emisión de gases de efecto invernadero. Chlorella spp., una microalga verde, presenta características promisorias para proveer nutrientes, especialmente nitrógeno, en dietas bajas en proteína. Sin embargo, existe información limitada relacionada con la inclusión de Chlorella spp. sobre la fermentación y la producción de metano (CH4) in vitro de la dieta. Objetivo: Evaluar el efecto de incrementar la inclusión de alga (Chlorella spp.) sobre el perfil de fermentación in vitro y la producción de CH4 de una dieta basada en ensilaje de maíz. Métodos: Las incubaciones fueron realizadas en tres días diferentes usando ensilaje de maíz y residuo de algodón como sustrato (en relación 70:30, respectivamente). Los tratamientos fueron: un tratamiento control (sin alga), e inclusiones de 1, 5 y 10% de alga en el sustrato. El fluido ruminal fue colectado de dos novillos mestizos Angus con cánula ruminal, alimentados con una dieta de ensilaje de maíz y residuo de algodón a voluntad. El pH final, la concentración de ácidos grasos volátiles (VFA) y nitrógeno amoniacal (NH3-N), la digestibilidad in vitro de la materia orgánica (IVOMD), y la producción de gas total y CH4 fueron determinadas después de 24 h de fermentación. Las variables fueron evaluadas usando el procedimiento MIXED del software SAS y las medias fueron comparadas usando contrastes de polinomios ortogonales. Resultados: Niveles crecientes de alga incrementaron (p<0,01) linealmente la IVOMD. Sin embargo, el pH final y la concentración de AGV y NH3-N no fueron diferentes (p>0,05) entre los niveles de alga. Además, las proporciones molares de VFA y la relación acetato:propionato no se afectaron con el incremento (p>0,05) en la concentración de alga. Finalmente, la producción de gas y de CH4 no fueron diferentes (p>0,05) entre tratamientos. Conclusión: La inclusión de Chlorella spp. no modifica la fermentación in vitro ni la producción de CH4 en una dieta basada en ensilaje de maíz.

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Biografía del autor/a

Juan de J Vargas, University of Florida

North Florida Research and Education Center. University of Florida. Marianna, FL, USA
https://orcid.org/0000-0002-7674-3850

Federico Tarnonsky, University of Florida

North Florida Research and Education Center. University of Florida. Marianna, FL, USA
https://orcid.org/0000-0002-5563-1956

Araceli Maderal, University of Florida

North Florida Research and Education Center. University of Florida. Marianna, FL, USA
https://orcid.org/0000-0003-2267-1131

Ignacio Fernández-Marenchino, University of Florida

North Florida Research and Education Center. University of Florida. Marianna, FL, USA
https://orcid.org/0000-0001-6712-9587

Federico Podversich, University of Florida

North Florida Research and Education Center. University of Florida. Marianna, FL, USA
https://orcid.org/0000-0003-0852-790X

Tessa M Schulmeister, University of Florida

North Florida Research and Education Center. University of Florida. Marianna, FL, U
https://orcid.org/0000-0002-8297-7442

Nicolás DiLorenzo, University of Florida

North Florida Research and Education Center. University of Florida. Marianna, FL, USA
https://orcid.org/0000-0001-5174-3427

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