Cinética de biohidrogenação ruminal in vitro dos ácidos linoleico e alfa-linolênico misturados
DOI:
https://doi.org/10.17533/udea.rccp.v31n3a06Palavras-chave:
ácidos graxos, cinética de biohidrogenação, suplemento lipídico, modelo multi-compartimentalizado, termodinâmica de biohidrogenaçãoResumo
Antecedêntes: o ácido linoleico (LA) e alfa-linolênico (LN) da dieta, são extensivamente isomerizados e biohidrogenados pelos microorganismos do rúmen, o que pode contribuir ao perfil de ácidos graxos dos produtos derivados de ruminantes. Objetivo: avaliar o efeito da relação LA:LN em suplementos lipídicos sobre a cinética de biohidrogenação ruminal do LA e LN como também sobre a produção do ácido transvaccênico (VA), utilizando um sistema in vitro. Métodos: coletou-se fluido ruminal de um novilho fistulado, o qual foi diluído com tampão de incubação e, em seguida, incubado com 500 mg de pasto kikuyu (Cenchrus clandestinus) suplementado com 16,3 mg de diferentes misturas LA:LN (100:0, 75:25 , 50:50, 25:75 ou 0:100). As incubações foram desenvolvidas em triplicata, durante 0, 2, 4, 6, 8 ou 16 horas. Diferenças entre tratamentos foram avaliadas utilizando-se um delineamento inteiramente casualizado. Alternativamente, foram determinadas as mudanças em energia livre de Gibbs (ΔGrxn) a 39 °C para as principais etapas da biohidrogenação do LA e LN, utilizando-se química computacional. Resultados: a substituição parcial de LA por LN diminuiu a concentração de VA e sua taxa de acumulação, como também aumentou a concentração de ácido esteárico e as taxas de transferência do LA para o ácido linoleico conjugado (CLA) e do CLA para VA. A conversão do CLA para VA (ΔGrxn = -2,65 kJ/ mol) foi mais espontânea que a conversão do ácido trans-11, cis-15 octadecadienóico (TA) para VA (ΔGrxn = -0,29 kJ/mol). Conclusões: a relação LA:LN em lipídeos pode modular a cinética de biohidrogenação (BH) do LA e LN e a produção de VA no rúmen.
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