Exploring bovine three-dimensional chondrocyte culture models in osteoarthritis research: A systematic review
DOI:
https://doi.org/10.17533/udea.rccp.357017Palavras-chave:
cartilagem, colheita de tecidos, cultura celular, hidrogel, obtenção de tecidos, osteoartrite, risco de viés, tecidos musculoesqueléticosResumo
Antecedentes: O uso de diferentes espécies animais para a cultura de condrócitos tem sido empregado para investigar doenças que afetam a cartilagem, incluindo osteoartrite. Cartilagem bovina e condrócitos podem ser usados para estabelecer culturas de células tridimensionais, que oferecem um modelo in vitro mais confiável em comparação com culturas convencionais de monocamadas. No entanto, condrócitos bovinos em culturas tridimensionais não foram amplamente implementados, faltando uma fonte potencial de tecido de mamíferos, o que poderia ser útil para estudos pré-clínicos de osteoartrite. Objetivo: Consequentemente, nosso objetivo foi realizar uma revisão abrangente da literatura científica existente empregando culturas tridimensionais de cartilagem bovina para investigar osteoartrite. Métodos: Foi realizada uma busca sistemática, utilizando as bases de dados eletrônicas PubMed e Scopus, para identificar estudos clínicos utilizando cultura de células 3D para osteoartrite. Os termos de pesquisa incluíram: ´3D culture’, ‘3D cell culture’, ‘bovine cartilage’ e ‘chondrocyte’. Foram coletados 59 artigos e, após seleção, 12 artigos foram incluídos na análise final. A avaliação do risco de viés foi realizada classificando-se cada um dos estudos em "baixo", "médio" ou "alto" risco de viés. Resultados: Verificamos que nos artigos incluídos nesta revisão houve alta variabilidade nos sítios de isolamento, incluindo as articulações do carpo, metacarpo e joelho, bem como alta variação nos métodos de cultura, utilizando passagens celulares que variam da passagem zero à passagem nove. Além disso, detectamos um risco médio e alto de viés em todos os artigos, provavelmente devido a dificuldades de randomização e cegamento dos estudos. Em resumo, esta revisão examina criticamente a cultura de células tridimensionais para a pesquisa de distúrbios da cartilagem, com ênfase particular na cartilagem bovina. Conclusões: Estudos futuros devem incluir métodos consistentes ao longo da fase in vitro do estudo, como locais de colheita uniformes, bem como o uso de passagens precoces de condrócitos para preservar o fenótipo celular. Além disso, a comparação de modelos translacionais relevantes deve incluir condições pareadas por idade para evitar mais fatores de confusão.
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