Evaluation of antimicrobial susceptibility of Salmonella Spp. strains isolated from pork carcasses on Colombia
DOI:
https://doi.org/10.17533/udea.rfnsp.14304Keywords:
nontyphoidal salmonellosis, pig saughter, food safety, antimicrobial resistance, Bauer & KirbyAbstract
Objective: this study was aimed to establish the patterns of antimicrobial resistance in 155 strains of Salmonella spp. isolated from pig carcasses in slaughter house in Colombia. Methodology: using the disc diffusion method (Bauer & Kirby, 1966) we evaluated nine antibiotics: amoxicillinclavulanic acid (30 ug), ampicillin (10 ug), ceftiofur (30 ug), ciprofloxacin (5 ug), chloramphenicol (30 ug), florfenicol (30 ug), gentamicin (10 ug), sulfadiazine / trimethoprim (25 ug) and tetracycline (30 ug). Results: the results confirm the presence of multiresistant strains of Salmonella spp. in pig slaughter house, showing 30 different patterns of multidrug resistance, the most common was ampicillin, amoxicillin, chloramphenicol, florfenicol and tetracycline in 16.77% (n = 26) strains. Tetracycline and florfenicol were less effective with 94.84% (n = 147) and 47.74% (n = 74) of the resistant strains, respectively. Discussion: it must consolidate monitoring programs and surveillance and control systems to prevent the spread of multiresistant strains in foods of animal origin, with an approach of supply chain of pork, as an essential element of food safety system in Colombia.
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References
(1). Multi-drug resistant Salmonellatyphimurium. Fact Sheet No 139. January 1997
(2). Gray JT, Fedorka-Cray PJ. Long-term survival and infectivity of Salmonellacholeraesuis. Berl Munch TierarztlWochenschr. 2001;114:370–4. DOI: https://doi.org/10.31274/safepork-180809-1162
(3). James J. Microbial Hazard Identification in Fresh Fruits and Vege-tables. Wiley Editorial; 2006. p. 46-9. DOI: https://doi.org/10.1002/0470007761
(4). Craig DE, Slauch JM. The Prokaryotes: The Genus Salmone-llaspp.Springer Editorial; 2006. p. 123–158. DOI: https://doi.org/10.1007/0-387-30746-X_7
(5). Cruchaga S, Echeita A, Aladueña A, García-Peña J, Frias N, et al.Antimicrobial resistance in Salmonellae from humans, food and animals in Spain in 1998. J Antimicrob Chemother. 2001;47:315-21. DOI: https://doi.org/10.1093/jac/47.3.315
(6). Varma JK, Greene KD, Ovitt J, Barrett TJ, MedallaF,et al. Hos-pitalization and antimicrobial resistance in Salmonella outbreaks, 1984–2002. Emerg Infect Dis. 2005;11:943–946. DOI: https://doi.org/10.3201/eid1106.041231
(7). Organización Mundial de la Salud, 1997; Organización Mundial de Sanidad Animal, 2001.INFOSAN: Resistencia Antimicrobiana a Salmonella. Nota de Información INFOSAN 3/2005.
(8). World Organisation for Animal Health (OIE). Terrestrial Animal Health Code. Harmonisation of national antimicrobial resistance surveillance and monitoring programmes. 2009. Chapter 6.8.
(9). Rajic ́A, McFall ME, Deckert AE, Reid-Smith R, Manninen K, Poppe C, et al. Antimicrobial resistance of Salmonella isolated from finishing swine and the environment of 60 Alberta swine farms. Vet Microbiol. 2004; 104: 189–196. DOI: https://doi.org/10.1016/j.vetmic.2004.09.013
(10). Hendriksen RS, Seyfarth AM, Jensen AB, Whichard J, Karlsmose S, Joyce K, et al. Results of Use of WHO Global Salm-Surv Ex-ternal Quality Assurance System for Antimicrobial Susceptibility Testing of SalmonellaIsolates from 2000 to 2007. J ClinMicro-biol. 2009; 47(1): 79–85. DOI: https://doi.org/10.1128/JCM.00894-08
(11). Bahnson, PB, Fedorka-Cray PJ, Ladely SR, Mateus-Pinilla NE. Herd-level risk factors for Salmonellaenterica subsp. enterica in U.S. market pigs. Prev. Vet. Med. 2006; 76: 249–262. DOI: https://doi.org/10.1016/j.prevetmed.2006.05.009
(12). Pasterán F, Galas M. Manual de Procedimientos Sensibilidad a los antimicrobianos en Salmonella, Shigella y E. coli . Departamento de Bacteriología Instituto Nacional de Enfermedades Infecciosas A.N.L.I.S. “Dr. Carlos G. Malbrán”. Centro Regional de Referen-cia WHO-Global SalmSurv para América del Sur. 2008.
(13). Clinical Laboratory and Standards Institute.Performance Stan-dards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated From Animals.Third Edition. 2008; M31-A3.
(14). World Organisation for Animal Healt (OIE). Manual of Diagnos-tic Tests and Vaccines for Terrestrial AnimalsResolution No. XX-VIII, 2007.
(15). Hendriksen R, Karlsmose S, Jensen AB, Aarestrup F. The Exter-nal Quality Assurance System of the WHO Global Salm-Surv, 2008. National Food Institute. Technical University of Denmark. [Documento en Internet]. 2009. [Acceso 22 de Febrero de 2012]. Disponible en http://www.food.dtu.dk/Publikationer.aspx
(16). Karczmarczyk M, Martins M, McCusker M, Mattar S, Amaral L, Leonard N, et al. Characterization of antimicrobial resistance in Salmonellaenterica food and animal isolates from Colombia: Identification of a qnrB19-mediated quinolone resistance marker in two novel serovars. FEMS MicrobiolLett. 2010; 313, 10–19. DOI: https://doi.org/10.1111/j.1574-6968.2010.02119.x
(17). Hur J, et al., Antimicrobial resistance of Salmonella isolated from food animals: A review.Food ResearchInternational. 2011;[In Press, Corrected Proof] doi:10.1016/j.foodres.2011.05.014. DOI: https://doi.org/10.1016/j.foodres.2011.05.014
(18). Zhao S, White DG, Friedman SL, Glenn A, Blickenstaff K, Ayers SL, et al. Antimicrobial Resistance in Salmonella entericaSerovar HeidelbergIsolates from Retail Meats, Including Poultry, from 2002 to 2006.Applied and Environmental Microbiology. (2008) 74(21): 6656–6662.. DOI: https://doi.org/10.1128/AEM.01249-08
(19). Foley SL, Lynne AM.Food animals-associated Salmonella cha-llenges: Pathogenicity and antimicrobial resistance. J Anim Sci. 2008; 86(E. Suppl.), E173–E187. DOI: https://doi.org/10.2527/jas.2007-0447
(20). Quinn PJ,Markey BK,Leonard FC,Carter MC, Donnelly WJ.Microbiología y Enfermedades Infecciosas Veterinarias. Acri-bia Editorial; 2000. Págs. 37 – 46
(21). Reunión conjunta FAO/OMS/OIE de expertos sobre los antimi-crobianos de importancia crítica.Informe de la reunión de exper-tos.Sede de la FAO, Roma (Italia)del 26 al 30 de noviembre de 2007
(22). Reunión conjunta FAO/OMS/OIE de expertos sobre los antimi-crobianos de importancia crítica.Informe de la reunión de exper-tos.Sede de la FAO, Roma (Italia)del 26 al 30 de noviembre de 2007
(23). Berends BR, Van Knapen F, Snijders JMA, MosselDA. Identifi-cation and quantification of risk factors regarding Salmonella spp. on pork carcasses.Int J Food Microbiol. 1997;36 (2-3,20):199-206. DOI: https://doi.org/10.1016/S0168-1605(97)01267-1
(24). Parra J, Polanco N, Rangel E, Arguello O, Flórez H, Rojas A. Ca-racterización nutricional, perfil lipídico y buenas prácticas gana-deras para mejorar la calidad de la leche cruda del Piedemonte Llanero para la Industria. Informe Final. 2010
(25). Phillips I, Casewell M, Cox T, De Groot B, Friis C, Jones R, et al. Does the use of antibiotics in food animals pose a risk to hu-man health? A critical review of published data.J AntimicrobChe-mother. 2004; 53: 28–52. DOI: https://doi.org/10.1093/jac/dkg483
(26). World Health Organization (WHO). Global principles for the con-tainment of antimicrobialresistance in animals intended for food.[Documento en Internet] 2000. [Acceso 22 de Febrero de 2012]. Disponible en: http://www.who.int/emc/diseases/zoo/who_glo-bal_principles.html.
(27). World Organisation for Animal Health (OIE). International Com-mittee of the OIE during its 77th General Session 24 – 29 May 2009. Resolution N° 24. Animal Production Food Safety.
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