Biocompatibility of bismuth silicate coatings deposited on 316L stainless steel by sol-gel process

Jorge Hernando Bautista-Ruiz; Jhon Jairo Olaya-Flórez; William Arnulfo  Aperador-Chaparro

doi:10.17533/udea.redin.n84a04

Authors

Jorge Hernando Bautista-Ruiz Francisco de Paula Santander University https://orcid.org/0000-0003-3430-2173
Jhon Jairo Olaya-Flórez National University of Colombia
William Arnulfo Aperador-Chaparro Nueva Granada Military University https://orcid.org/0000-0003-1778-0851

DOI:

https://doi.org/10.17533/udea.redin.n84a04

Keywords:

MTT assay, bismuth silicate films, sol-gel, biocompatibility

Abstract

Bismuth silicate, (BSO) thin films have been fabricated by sol–gel process. The stable sol was synthesized by using bismuth nitrate (III) pentahydrate and tetraethyl orthosilicate (TEOS). The films were deposited by spin-coating at 1500 rpm on Stainless Steel 316L. The films were characterized by Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM), X-ray diffraction (XRD) besides the measured of the films thickness. This study aimed to evaluate the cell adhesion and cellular proliferation of osteoblast cells on BSO thin films and substrate 316L by MTT assay. It is concluded that the growth of osteoblasts is homogeneous in the surface of the coatings indicating that the medium offered by the films does not present active cytotoxicity and exceed in large number the level of cellular growth compared to Stainless Steel.

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Author Biographies

Jorge Hernando Bautista-Ruiz, Francisco de Paula Santander University

Department of Physics, Faculty of Basic Sciences.

Jhon Jairo Olaya-Flórez, National University of Colombia

Faculty of Engineering.

William Arnulfo Aperador-Chaparro, Nueva Granada Military University

Faculty of Engineering.

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