effect of silver diamine fluoride on caries induced in Wistar rats

Authors

  • Susana Vanegas Universidad de Los Andes
  • Astrid Godoy Universidad de Los Andes
  • Leonidas Urdaneta Universidad de Los Andes
  • Daniela Olávez Universidad de Los Andes
  • Karla Padrón Universidad de los Andes
  • Eduvigis Solórzano Universidad de Los Andes

DOI:

https://doi.org/10.17533/udea.rfo.15953

Keywords:

Dental caries, Primary teeth, Silver Diamine Fluoride (SDF), Animal model

Abstract

Introduction: dental caries is considered a public health problem. Various biomaterials have been suggested for treating it, including fluride+silver nitrate mix, known as silver diamine fluoride (SDF) which contains cariostatic, remineralizing, and bactericide properties and is used on primary teeth. Only a few studies have assessed the response of dental tissue when this substance is applied; the objective of this study was therefore to determine the effect of SDF on caries induced in Wistar rats. Methods: dental caries was induced in Wistar rats’ molars by inoculating them with S. mutans CVCM656 and feeding them a sucrose-rich diet for 12 weeks. Once caries were diagnosed and sorted out into control groups (C1, C2) and experimental groups (E1, E2), softened dentine was removed from molars; only the molars from experimental groups received a topical application of 3.8% SDF. Finally, clinical assessment was performed and samples were collected for histological study 7 and 13 weeks after treatment. Results: the control groups experienced an increased number of carious lesions and progression in severity, while the same parameters in the experimental groups remained unchanged, showing hard dark dentine surfaces. The histological report showed increased predentine thickness in molars from the experimental groups only. Conclusions: the protocol of caries induction in Wistar rats was successful. Treatment with SDF deactivated the carious processes, arresting not only severity progression but also the increase in number of caries; these findings may be extrapolated to humans.

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Author Biographies

Susana Vanegas, Universidad de Los Andes

DMD. Universidad de Los Andes, Mérida, Venezuela. Dentist in private practice

Astrid Godoy, Universidad de Los Andes

DMD. Universidad de Los Andes, Mérida, Venezuela. Dentist in private practice 

Leonidas Urdaneta, Universidad de Los Andes

BA in Bioanalysis. Magister Scientiae in Clinical Microbiology, Biopatology Research/Comprehensive Laboratory of Cellular and Molecular Biology Research Group. Adjunct Microbiology Professor, School of Dentistry, Universidad de Los Andes. Mérida, Venezuela.

Daniela Olávez, Universidad de Los Andes

DMD. Biopatology Research/Comprehensive Laboratory of Cellular and Molecular Biology Research Group. Adjunct Histology Professor, School of Dentistry, Universidad de Los Andes. Mérida, Venezuela

Karla Padrón, Universidad de los Andes

DMD. Biopatology Research/Comprehensive Laboratory of Cellular and Molecular Biology Research Group. Adjunct Histology Professor, School of Dentistry, Universidad de Los Andes. Mérida, Venezuela

Eduvigis Solórzano, Universidad de Los Andes

DMD. PhD in Biologic Anthropology. Biopatology Research/Comprehensive Laboratory of Cellular and Molecular Biology Research Group. Adjunct Histology Professor, School of Dentistry, Universidad de Los Andes. Mérida, Venezuela.

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Published

2014-07-14

How to Cite

Vanegas, S., Godoy, A., Urdaneta, L., Olávez, D., Padrón, K., & Solórzano, E. (2014). effect of silver diamine fluoride on caries induced in Wistar rats. Revista Facultad De Odontología Universidad De Antioquia, 26(1), 76–88. https://doi.org/10.17533/udea.rfo.15953

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