Contamination of -percha cones in clinical use by endodontic specialists and general practitioners
DOI:
https://doi.org/10.17533/udea.rfo.v28n2a6Keywords:
Bacterial contamination, Endodontic infection, Gutta-percha, Endodontic treatment, Root canalAbstract
Introduction: the present study evaluated the microbial contamination of gutta-percha cones proceeding from packages used clinically by endodontic specialists and general practitioners. Methods: two gutta-percha cones were selected from 30 original packages, already in clinical use, in dental clinics. The cones were transferred directly to test tubes containing thioglycolate broth and incubated at 37 °C for 21 days in aerobiosis. All tests were done in triplicate. Fractions proceeding from the tubes that presented turbidity were plated in CLED agar and Gram staining. Results: Among the gutta-percha cone boxes tested, 9 (30%) showed bacterial contamination in the tested cones, 4 (13%) of those coming from general practitioners and 5 (17%) coming from specialists. There was no significant difference in the contamination of cones in relation to their origin (p>0,05). Conclusion: The results of the present study reinforce the need for both clinical dentists and endodontics specialists to implement a strict disinfection protocol before using gutta-percha cones, due to the frequency of contamination
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References
Sundqvist G, Figdor D, Persson S, Sjögren U. Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative re-treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1998; 85(1): 86-93.
Kakehashi S, Stanley HR, Fitzgerald RJ. The effects of surgical exposures of dental pulps in germ-free and conventional laboratory rats. Oral Surg Oral Med Oral Pathol 1965; 20: 340-349.
Sundqvist G. Bacteriological studies of necrotic dental pulps. Dissertation. Umea, Sweden: University of Umea; 1976.
Siqueira JF Jr. Aetiology of root canal treatment failure: why well-treated teeth can fail. Int Endod J 2001; 34(1): 1-10.
Vianna ME, Horz HP, Conrads G, Zaia AA, Souza-Filho FG, Gomes BP. Effect of root canal procedures on endotoxins and endodontic pathogens. Oral Microbiol Immunol 2007; 22(6): 411-418. DOI: 10.1111/j.1399-302X.2007.00379.x URL: https://doi.org/10.1111/j.1399-302X.2007.00379.x
Gomes BP, Vianna ME, Matsumoto CU, Rossi-Vde P, Zaia AA, Ferraz CC et al. Disinfection of gutta-percha cones with chlorhexidine and sodium hypochlorite. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2005; 100(4): 512-517. DOI: 10.1016/j.tripleo.2004.10.002 URL: https://doi.org/10.1016/j.tripleo.2004.10.002
Seabra-Pereira OL, Siqueira JF Jr. Contamination of gutta-percha an Resilon cones taken directly from the manufacturer. Clin Oral Investig 2010; 14(3): 327-330. DOI: 10.1007/s00784-009-0295-z URL: https://doi.org/10.1007/s00784-009-0295-z
Kayaoglu G, Gürel M, Omürlü H, Bek ZG, Sadik B. Examination of gutta-percha cones for microbial contamination during chemical use. J Appl Oral Sci 2009; 17(3): 244-247.
Siqueira JF Jr, Silva CH, Cerqueira MC, Lopes HP, de Uzeda M. Effectiveness of four chemical solutions in eliminating Bacillus subtilis spores on gutta-percha cones. Endod Dent Traumatol 1998; 14(3): 124-126:
Siqueira JF Jr, Rôças IN. Update on endodontic microbiology: candidate pathogens and patterns of colonisation. ENDO 2008a; 2(1): 7-20.
Siqueira JF Jr, Rôças IN. Clinical implications and microbiology of bacterial persistence after treatment procedures. J Endod 2008b; 34(11): 1291-1301. DOI: 10.1016/j.joen.2008.07.028 URL: https://doi.org/10.1016/j.joen.2008.07.028
Torabinejad M, Kutsenko D, Machnick TK, Ismail A, Newton CW. Levels of evidence for the outcome of nonsurgical endodontic treatment. J Endod 2005; 31(9): 637-646.
Schilder H. Filling root canals in three dimensions. J Endod 2006; 32(4): 281-290. DOI: 10.1016/j.joen.2006.02.007 URL: https://doi.org/10.1016/j.joen.2006.02.007
Friedman CM, Sandrik JL, Heuer MA, Rapp GW. Composition and mechanical properties of gutta-percha endodontic points. J Dent Res 1975; 54(5): 921-925. DOI: 10.1177/00220345750540052901 URL: https://doi.org/10.1177/00220345750540052901
Friedman CE, Sandrik JL, Heuer MA, Rapp GW. Composition and physical properties of gutta-percha endodontic filling materials. J Endod 1977; 3(8): 304-308. DOI: 10.1016/S0099-2399(77)80035-6 URL: https://doi.org/10.1016/S0099-2399(77)80035-6
Moorer WR, Genet JM. Evidence for antibacterial activity of endodontic gutta-percha cones. Oral Surg Oral Med Oral Pathol 1982; 53(5): 503-507.
Montgomery S. Chemical decontamination of gutta-percha cones with polyvinylpyrrolidone-iodine. Oral Surg Oral Med Oral Pathol 1971; 31(2): 258-266.
Podbielski A, Boeckh C, Haller B. Growth inhibitory activity of gutta-percha points containing root canal medications on common endodontic bacterial pathogens as determined by an optimized quantitative in vitro assay. J Endod 2000; 26(7): 398-403. DOI: 10.1097/00004770-200007000-00005 URL: https://doi.org/10.1097/00004770-200007000-00005
Lui JN, Sae-Lim V, Song KP, Chen NN. In vitro antimicrobial effect of chlorhexidine-impregnated gutta-percha points on Enterococcus faecalis. Int Endod J 2004; 37(2): 105-113.
Chogle S, Mickel AK, Huffaker SK, Neibaur B. An in vitro assessment of iodoform gutta-percha. J Endod 2005; 31(11): 814-816.
Higgins JR, Newton CW, Palenik CJ. The use of paraformaldehyde powder for the sterile storage of gutta-percha cones. J Endod 1986; 12(6): 242-248. DOI: 10.1016/S0099-2399(86)80255-2 URL: https://doi.org/10.1016/S0099-2399(86)80255-2
Namazikhah MS, Sullivan DM, Trnavsky GL. Gutta-percha: a look at the need for sterilization. J Calif Dent Assoc 2000; 28(6): 427-432.
Anbu R, Nandini S, Velmurugan N. Volumetric analysis of root fillings using spiral computed tomography: an in vitro study. Int Endod J 2010; 43(1): 64-68. DOI: 10.1111/j.1365-2591.2009.01638.x URL: https://doi.org/10.1111/j.1365-2591.2009.01638.x
James BL, Brown CE, Legan JJ, More BK, Bail MM. An in vitro evaluation of the contents of root canals. J Endod 2007; 33(11): 1359-1363. DOI: 10.1016/j.joen.2007.07.021 URL: https://doi.org/10.1016/j.joen.2007.07.021
da-Motta PG, de-Figueiredo CB, Maltos SM, Nicoli JR, Ribeiro-Sobrinho AP, Maltos KL et al. Efficacy of chemical sterilization and storage conditions of gutta-percha cones. Int Endod J 2001; 34(6): 435-439.
Attin T, Zirkel C, Pelz K. Antibacterial properties of electron beam sterilized gutta-percha cones. J Endod 2001; 27(3): 172-174. DOI: 10.1097/00004770-200103000-00006 URL: https://doi.org/10.1097/00004770-200103000-00006
Siqueira JF Jr, Rôças IN. Exploiting molecular methods to explore endodontic infections: Part I- Current molecular technologies for microbial diagnosis. J Endod 2005; 31(6): 411-423.
Nabeshima CK, Machado ME, Britto ML, Pallotta RC. Effectiveness of different chemical agents for disinfection of gutta-percha cones. Aust Endod J 2011; 37(3): 118-121. DOI: 10.1111/j.1747-4477.2010.00256.x URL: https://doi.org/10.1111/j.1747-4477.2010.00256.x
Pang NS, Jung IY, Bae KS, Baek SH, Lee WC, Kum KY. Effects of short-term chemical disinfection of gutta-percha cones: identification of affected microbes and alterations in surface texture and physical properties. J Endod 2007; 33(5): 594-608. DOI: 10.1016/j.joen.2007.01.019 URL: https://doi.org/10.1016/j.joen.2007.01.019
Subha N, Prabhakar V, Koshy M, Abinaya K, Prabu M, Thangavelu L. Efficacy of peracetic acid in rapid disinfection of Resilon and gutta-percha cones compared with sodium hypochlorite, chlorhexidine, and povidone-iodine. J Endod 2013; 39(10): 1261-1264. DOI: 10.1016/j.joen.2013.06.022. URL: https://doi.org/10.1016/j.joen.2013.06.022.
Marciano J, Michailesco PM, Abadie MJ. Stereochemical structure characterization of dental gutta-percha. J Endod 1993; 19(1): 31-34.
Marciano J, Michailesco PM. Dental gutta-percha: chemical composition, X-ray identification, enthalpic studies, and clinical implications. J Endod 1989; 15(4): 149-153. DOI: 10.1016/S0099-2399(89)80251-1 URL: https://doi.org/10.1016/S0099-2399(89)80251-1
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