Contamination of -percha cones in clinical use by endodontic specialists and general practitioners




Bacterial contamination, Endodontic infection, Gutta-percha, Endodontic treatment, Root canal


Introduction: the present study evaluated the microbial contamination of gutta-percha cones proceeding from packages used clinically by endodontic specialists and general practitioners. Methods: two gutta-percha cones were selected from 30 original packages, already in clinical use, in dental clinics. The cones were transferred directly to test tubes containing thioglycolate broth and incubated at 37 °C for 21 days in aerobiosis. All tests were done in triplicate. Fractions proceeding from the tubes that presented turbidity were plated in CLED agar and Gram staining. Results: Among the gutta-percha cone boxes tested, 9 (30%) showed bacterial contamination in the tested cones, 4 (13%) of those coming from general practitioners and 5 (17%) coming from specialists. There was no significant difference in the contamination of cones in relation to their origin (p>0,05). Conclusion: The results of the present study reinforce the need for both clinical dentists and endodontics specialists to implement a strict disinfection protocol before using gutta-percha cones, due to the frequency of contamination

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Author Biographies

Marcia Cristina André Moreira-Nacif, Estacio de Sá University

DDS, MSc in Endodontics, Estácio de Sá University, Rio de Janeiro, Brazil

Marilia Fagury Videira Marceliano-Alves, Estacio de Sá University

DDS, MSc, PhD Post Graduation Program in Endodontics at Estácio de Sá University, Rio de Janeiro, Brazil

Flavio Rodrigues Ferreira-Alves, Estacio de Sá University

DDS, MSc, PhD Post Graduation Program in Endodontics at Estácio de Sá University, Rio de Janeiro, Brazil 


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How to Cite

Moreira-Nacif, M. C. A., Marceliano-Alves, M. F. V., & Ferreira-Alves, F. R. (2017). Contamination of -percha cones in clinical use by endodontic specialists and general practitioners. Revista Facultad De Odontología Universidad De Antioquia, 28(2), 327–340.

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