Effects of corn oil on the gastric mucosa of horses with induced ulcer
equine gastric ulcer syndrome (EGUS) has a multifactorial nature and it affects both the squamous and glandular mucosa of the stomach. Multiple therapeutic strategies involving large periods of medication are used to treat this condition. Objective: to evaluate the effects of administering corn oil (CO) to horses with induced gastric ulcers, and to describe the mechanism of action of the mucosal repair. Methods: fifteen horses divided into three groups were used. A combination of confinement and phenylbutazone was used during the ulcer-induction phase. After the gastroscopic evaluation and laboratory tests, animals were treated with sucralfate (SA; group I), and CO at doses of 70 and 90 mL/100 Kg (groups II and III, respectively). Gastroscopy, gastric content collection, and biopsy of the glandular mucosa were performed to analyze prostaglandin E2 (PGE2), pH, and antioxidant and oxidant parameters. Results: mild to moderate gastric lesions were induced in the glandular and non-glandular mucosa. Among the mechanisms of the treatments, reestablishment of the antioxidant parameters and inhibition of myeloperoxidase (MPO) oxidant enzyme were prominent, but PGE2 concentration had a weak influence. Conclusion: similarly to SA, CO only had therapeutic effects in the glandular mucosa.
Abdallah DM. Nicotinamide alleviates indomethacin-induced gastric ulcers: A novel antiulcer agent. Eur J Pharmacol 2010; 627:276-280. Aebi H. Catalase in vitro. Method Enzymol 1984; 105:121-126. Andrews F, McConnico R. Cause for concern: evidence that therapeutic dosing of nonselective NSAID contributes to gastrointestinal injury. Equine Vet Educ 2009; 21:663-664. Basiviredy J, Jacob M, Ramamoorthy P, Pulimood A, Balasubramanian K. Indomethacin-induced free radical mediate changes in the intestinal brush border membranes. Biochem Pharmacol 2003; 65:683-695. Beall CM, Laskowski D, Erzurum SC. Nitric oxide in adaptation to altitude. Free Radical Biol Med 2012; 52:1123-1134. Belo AV, Barcelos LS, Ferreira M, Texeira MM, ANDRADE SP. Inhibition of inflammatory angiogenesis by distant subcutaneous tumor in mice. Life Sciences 2004; 74:2827-2837. Cargile JL, Burrow JA, Kim I, Cohen ND, Merritt AM. Effect of dietary corn oil supplementation on equine gastric fluid acid, sodium, and prostaglandin E2 content before and during pentagastrin infusion. J Vet Intern Med 2004; 18:545-549. Carollo M, Hogaboam C, Kunkel S, Delaney S, Christie M, Perretti M. Analysis of the temporal expression of chemokines and chemokines receptors during experimental granulomatous inflammation: role and expression of MIP-1α and MCP-1. Brit J Pharmacol 2001; 134:1166-1179. Cicerale S, Lucas LJ, Keast RS. Antimicrobial, antioxidant and anti-inflammatory phenolic activities in extra virgen olive oil. Curr Opinion Biotechnol 2012; 23:129-135. Cryer B. Mucosal defense and repair: role of prostaglandins in the stomach and duodenum. Gastroenterol Clin N Am 2001; 30: 877-894.Del Rio D, Stewart AJ, Pellegrini N. A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutr Metab Cardiovasc Dis 2005; 15:316-328. Dieterich S, Bieligk U, Beulich K. Gene expression of antioxidative enzymes in the human heart: increased expression of catalase in the end-stage failing heart. Circulation 2000; 101:33-39. Frank N, Andrews FM, Elliott SB, Lew BS. Effects of dietary oils on the development of gastric ulcers in mares. Am J Vet Res 2005; 66:2006-2011. Grant HW, Palmer KR, Kelly RW, Wilson NH, Misiewicz JJ. Dietary linoleic acid, gastric acid, and prostaglandin secretion. Gastroenterology 1988; 94:955-959. Gulcan E, Keles M, Uyanik A. Can sucralfate be effective to protect on peritoneal membrane in patients receiving peritoneal dialysis treatment?. Med Hypotheses 2012; 78:415. Hecker M, Ullrich V. On the mechanism of prostacyclin and thromboxane A2 biosynthesis. J Biol Chem 1989; 264:141-150. Jonsson H, Egenvall A. Prevalence of gastric ulceration in Swedish Standardbred in race-training. Equine Vet J 2006; 38:209-213. Junior TR, Rezende AS, Junior OV, Bretas M, Lana A, Moura RS, Resende HC. Efeito do nível de óleo de milho adicionado à dieta de eqüinos sobre a digestibilidade dos nutrientes. Arq Bras Med Vet Zootec 2003; 56:69-73. Karatas F, Karatepe M, Baysar A. Determination of free malondialdehyde in human serum by high-performance liquid chromatography. Anal Biochem 2002; 311:76-79. Kobayashi K, Arakawa T. Arachidonic acid cascade and gastric mucosal injury, protection, and healing: topics of this decade. J Clin Gastroenterol 1995; 21:12-17. Lastra CA, Barranco MD, Martin MJ. Extra-virgin olive oil-enriched diets reduce indomethacine induced gastric oxidative damage in rats. Dig Dis Sci 2002; 47:2783-2790. Luthersson N, Nielsen KH, Harris P. Risk factors associated with equine gastric ulceration syndrome (EGUS) in 201 horses in Denmark. Equine Vet J 2009; 41:625–630. MaCallister CG, Morgan SJ, Borne AT. Comparison of adverse effects of phenylbutazone, flunixin meglumine and ketoprofen in horses. J Am Vet Med Ass 1993; 202: 71-77. MacAllister CG, Andrews FM, Deegan E, Ruoff W, Olovson SG. A scoring system for gastric ulcers in the horse. Equine Vet J 1997; 29:430-433. Martínez Aranzales JR, Cândido de Andrade BS, Silveira Alves GE. Orally administered phenylbutazone causes oxidative stress in the equine gastric mucosa. J Vet Pharmacol Therap 2015; 38(3):257-264.
Miranda KM, Espey MG, Wink DA. A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide 2001; 5:62-71. Moreno JJ. Effect of olive oil minor components on oxidative stress and arachidonic acid mobilization and metabolism by macrophases raw 264.7. Free Radic Biol Med 2003; 35:1073-1081. Morrissey NK, Bellenger CR, Ryan MT, Baird AW. Cyclooxygenase-2 mRNA expression in equine nonglandular and glandular gastric mucosal biopsy specimens obtained before and after induction of gastric ulceration via intermittent feed deprivation. Am J Vet Res 2010; 71:1312-1320. Naito Y, Yoshikawa T, Yoshida N, Kondo M. Role of oxygen radical and lipid peroxidation in indomethacin-induced gastric mucosal injury. Dig Dis Sci 1998; 43:30-34. Nieto JE, Aleman M, Anderson JD, Fiack C, Snyder JR. Effects of phenylbutazone on gene expression of cyclooxygenase-1 and -2 in the oral, glandular gastric, and bladder mucosae of healthy horses. Am J Vet Res 2012; 73:98-104. Odabasoglu F, Cakir A, Suleyman H, Aslam A, Bayir Y, Halici M, Kazaz C. Gastroprotective and antioxidant effects of usnic acid on indomethacin-induced gastric ulcer rats. J Ethnopharmacol 2008; 103:59-65. Onyango AN, Baba N. New hypotheses on the pathways of formation of malondialdehyde and isofurans. Free Radic Biol Med 2010; 49:1594-1600. Orsini JA, Hackett E, Grenager N. The effect of exercise on equine gastric ulcer syndrome in the Thoroughbred and Standardbred athlete. J Equine Vet Sci 2009; 29:167-171. Polat B, Suleyman H, Hakan AH. Adaptation of rat gastric tissue against indomethacin toxicity. Chem Biol Interact 2010; 186:82-89. Rocha GA, Queiroz DM, Mendes EN, Lage AP, Barbosa AJA. Simple carbolfucsin staining for showing C. pylori and other spiral bacteria in gastric mucosa. J Clin Pathol 1989; 42:1004-1005. Sammon AM, Iputo JE. Maize meal predisposes to endemic squamous cancer of the oesophagus in Africa: breakdown of esterified linoleic acid to the free form in stored meal leads to increased intragastric PGE2 production and low-acid reflux. Med Hypotheses 2006; 67:1430-1435. Sanchez S, Martin MJ, Ortiz P, Motilva V, Lastra CA. Effects of dypirone on inflammatory infiltration and oxidative metabolism in gastric mucosa: comparison with acetaminophen and diclofenaco. Dig Dis Sci 2002; 47:1389-1398. Speirs VC. The alimentary Tract. In: Robinson NE, editor. Clinical Examination of horses. Philadelphia: WB Sounders; 1997. p. 261-298.Tamzali Y, Marguet C, Priymenko N, Lyazrhi F. Prevalence of gastric ulcer syndrome in high-level endurance horses. Equine Vet J 2011; 43: 141-144. Tomisato W, Tsutsumi S, Hoshino T, Hwang HJ, Mio M, Tsuchiya T, Mizushima T. Role of direct cytotoxic effects of NSAID in the induction of gastric lesions. Bioch Pharmac 2004; 67: 575-585. Tomlinson J, Blikslager A. Role of nonsteroidal anti-inflammatory drugs in gastrointestinal tract injury and repair. J Am Vet Med Ass 2003; 222: 946-951. Visioli F, Poli A, Gall C. Antioxidant and other biological activities of phenols from olives and olive oil. Med Res Rev 2002; 22:66-75. Wallace JL, Miller MJ. Nitric oxide in mucosal defense: A little goes a long way. Gastroenterology 2000; 119:512-520.
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