Caracterização e resistência antimicrobiana de Moraxella ovis isolados de casos clínicos de ceratoconjuntivite contagiosa ovina no Estado do México, México
DOI:
https://doi.org/10.17533/udea.rccp.v37n1a4Palavras-chave:
cabra, genes de resistência, Moraxella spp., multirresistência, ovelha, ceratoconjuntivite contagiosa ovina, resistência antimicrobiana, vigilância epidemiológicaResumo
Antecedentes: A ceratoconjuntivite contagiosa ovina (OKC) é uma doença infeccioso contagioso que causa cegueira temporária ou permanente em ovinos e caprinos, esta doença está associada a um conjunto de gêneros bacterianos dos quais alguns deles relataram resistência antimicrobiana. Objetivo: O objetivo deste estudo foi identificar a relação fenotípica-genotípica da resistência antimicrobiana de Moraxella spp. isolados obtidos de casos clínicos de ceratoconjuntivite contagiosa ovina (OKC) no estado do México. Métodos: Um total de 209 amostras foram obtidas de casos clínicos de OKC em ovinos.e obtidos e 60 isolados de Moraxella ovis foram identificados por técnicas bacteriológicas e amplificação dos genes 16s rRNA e rtxA por PCR. Todos os isolados foram avaliados quanto à resistência antimicrobiana pelo método de teste de suscetibilidade à difusão em disco e pela amplificação de genes de resistência por PCR respectivamente. Resultados: Determinamos 14 isolados de Moraxella ovis com resistência antimicrobiana (AMR) e cinco multirresistentes (MDR) e amplificou os genes de resistência antimicrobiana sul1, sul2, tetB, qnrA, qnrB, BlaTEM e não amplificou o gene floR. Conclusão: É o primeiro relato de isolamento de Moraxella. ovis em lesões oculares em ovinos no Estado do México e a identificação de seis genes de resistência antimicrobiana. Sugere-se que Moraxella ovis. desempenha um papel importante no curso da doença e fornece um panorama de interesse em vigilância epidemiológica molecular e resistência bacteriana.
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