VALIDATION OF AN ANALYTICAL METHODOLOGY TO QUANTIFY CYANOGENIC GLUCOSIDES IN COMMERCIAL CASSAVA PRODUCTS

Authors

  • Darwin ORTIZ
  • Teresa SÁNCHEZ
  • Harold ACOSTA
  • Helena PACHÓN

DOI:

https://doi.org/10.17533/udea.vitae.10681

Keywords:

Cyanogenic glucosides, cassava, hydrogen cyanide, validation methods, spectrophotometry.

Abstract

A spectrophotometric method for determining cyanogenic glucosides (CGs) was validated in accordance with IUPAC and ICH requirements for new methods. The method demonstrates good linearity in the range of 0.1-0.88 μgg-1 hydrocyanic acid (HCN). The accuracy of the method ranges between 95.2% and 96.4%. The precision of the method, reflected as the relative standard deviation of the replicates, is lower than 12%. The method was compared with the most used method to detect CGs in cassava roots, the one established by Essers et al. Both methods were applied to quantify CGs in commercial cassava products. The new method is a useful and reliable tool for monitoring levels of CGs in cassava commercial products.
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Published

24-11-2011

How to Cite

ORTIZ, D., SÁNCHEZ, T., ACOSTA, H., & PACHÓN, H. (2011). VALIDATION OF AN ANALYTICAL METHODOLOGY TO QUANTIFY CYANOGENIC GLUCOSIDES IN COMMERCIAL CASSAVA PRODUCTS. Vitae, 18(3), 319–324. https://doi.org/10.17533/udea.vitae.10681

Issue

Section

Natural Products