Antimicrobial activity of propolis and its effect on the physicochemical and sensorial characteristics in sausages
DOI:
https://doi.org/10.17533/udea.vitae.12795Keywords:
Propolis, antioxidant, decarboxylation, sensorial characteristics, sausageAbstract
Background: In the food industry, the use of natural additives capable of replacing chemical additives is increasing due to the tendency to consume natural and healthy foods. In nature, different substances such as propolis can fulfill that role and it is obtained from hives of honey bees. Propolis contains bioactive compounds with antimicrobial and antioxidant capacity, and thus, they could be used as an alternative for nitrites in meat products. Objectives : To evaluate the in-vitro antimicrobial activity of the ethanol propolis extract (EEP) of on some pathogenic bacteria and their influence on the physicochemical and sensorial properties of sausages. Methods: The ethanol extraction was performed, and its antimicrobial activity in vitro on S. aureus, Salmonella, E. coli and Clostridium spp. was determined. Sausages were prepared according to the following treatments: (1) EEP 0.8mg/mL, (2) 0.2g/Kg sodium nitrite and sodium erythorbate, (3) Alcohol (96% v/v used as control) and the physicochemical analysis was performed to determine thiobarbituric acid (TBA), volatile nitrogen bases (TVB - N) and sensoral tests every week for four weeks. Analysis of variance and Tukey test were performed and the level of significance was p < 0.05. Results: No significant differences between EEP concentration, pH and TVB-N or sensorial properties (p > 0.05) were found. However, significant differences in TBA values were observed (p < 0.05). Conclusions: A 0.8% EEP shows antimicrobial activity against some pathogenic microorganisms evaluated. Further, the physicochemical and sensorial characteristics of the product do not differ from the sausages containing nitrites.
Downloads
References
Fernández J, Pérez-Álvarez JA, Fernández-López JA. Thiobarbituric acid test for monitoring lipid oxidation in meat. Food Chemistry. 1997; 59(3): 345-53.
Honikel K-O. The use and control of nitrate and nitrite for the processing of meat products Meat Science. 2008; 78(1–2): 68-76.
Pegg RB, Shahidi F. Nitrite curing of meat: the N-nitrosamine problem and nitrite alternatives: Food & Nutrition Press; 2004.
Duarte MCT, Leme EE, Delarmelina C, Soares AA, Figueira GM, Sartoratto A. Activity of essential oils from Brazilian medicinal plants on Escherichia coli. Journal of Ethnopharmacology. 2007; 111(2): 197-201.
Lv F, Liang H, Yuan Q, Li C. In vitro antimicrobial effects and mechanism of action of selected plant essential oil combinations against four food-related microorganisms. Food Research International. 2011; 44(9): 3057-64.
Rojas-Graü MA, Avena-Bustillos RJ, Olsen C, Friedman M, Henika PR, Martín-Belloso O, et al. Effects of plant essential oils and oil compounds on mechanical, barrier and antimicrobial properties of alginate–apple puree edible films. Journal of Food Engineering. 2007; 81(3): 634-41.
Fernandez-Saiz P, Lagaron JM, Ocio MJ. Optimization of the biocide properties of chitosan for its application in the design of active films of interest in the food area. Food Hydrocolloid. 2009; 23(3): 913-21.
Gómez-Estaca J, López de Lacey A, López-Caballero ME, Gómez-Guillén MC, Montero P. Biodegradable gelatin–chitosan films incorporated with essential oils as antimicrobial agents for fish preservation. Food Microbiology. 2010; 27(7): 889-96.
Ahn M-R, Kumazawa S, Usui Y, Nakamura J, Matsuka M, Zhu F, et al. Antioxidant activity and constituents of propolis collected in various areas of China. Food Chemistry. 2007; 101 (4): 1383-92.
Sebranek JG, Bacus JN. Cured meat products without direct addition of nitrate or nitrite: what are the issues? Meat Science. 2007; 77 (1):136-47.
Zhang X, Kong B, Xiong YL. Production of cured meat color in nitrite-free Harbin red sausage by Lactobacillus fermentum fermention. Meat Science. 2007; 77 (4): 593-8.
Downloads
Published
How to Cite
Issue
Section
License
Copyright Notice and Open Access Statement
The Journal Vitae works under the Open Access license, and the published manuscripts remain available for the public, both on the Journal's website and in databases, under the Creative Commons license, "Noncommercial Attribution" and "Share alike" systems, adopted in Colombia. Hence, when the authors agree to publish in the Journal Vitae, they will not have the right to economic retributions on publications and reproductions through different diffusion media. The documents are freely available to the internet public, permitting users to read, download, copy, distribute, print, search, or link to the full texts and pass them as data to software. The only constraint on reproduction and distribution, should be to give authors control over the integrity of their work and the right to be appropriately acknowledged and cited.
Authors declare that:
-
They are the intellectual property owners and are responsible for all the information stated in the article.
-
This manuscript has not been submitted or published in other printed or digital media. They accept the responsibility for the judgments, opinions, and points of view expressed in the published article and, therefore, they exonerate Universidad de Antioquia and Journal Vitae from any process.
-
They exempt Universidad de Antioquia and Journal Vitae from settling conflicts or disputes related to the authorship of the referred article.
-
They accept the revision of the original manuscript by suitable personnel, and they bind themselves to perform the corrections appointed or suggested by the assessors.
-
Therefore, they know the editorial process and will not bind the Editorial Board of the Journal to assume any obligations regarding the volume and issue in which the article is published.
-
They transfer the rights of publication, reprinting, and distribution of the article from the moment of its approval, in print and digital format, without the right to economic rewards, and under the licensing conditions considered relevant by Journal Vitae.
-
They fully authorize Universidad de Antioquia and Journal Vitae to submit the published material to the diverse databases and indexing systems where the Journal can be found to comply with the requirements of the regulatory authorities to maintain the national classification of journals.
-
They will assume the article publication costs established for the current issue, and they will make the payment as soon as they are informed about the volume and the issue in which the final version of the article is published.
-
After the article is published, you can share digital or printed copies in a noncommercial manner. You will be able to use the paper in your institution or company for educational or research purposes, including the use in course programs.
Conflict of interest: Authors are responsible for recognizing and disclosing any financial or other benefits that could be perceived to bias their work, acknowledging all financial support and any personal connections with potential sponsors. Examples of such conflicts include receiving research funds or honoraria, serving on advisory boards, stock ownership, or employment and consulting arrangements. Authors without such connections should clearly state that they have no financial support or personal relationships that could be perceived to bias their work. All conflicts of interest should be disclosed on the author's identification page of the manuscript.
