Analgesic and neuromotor effects of two extracts of Zanthoxylum rhoifolium (Rutaceae) in the Swiss albino mouse (Mus musculus)


  • Melany Villegas-Valencia Pontificia Universidad Javeriana.
  • Libardo Andrés González-Reyes Universidad del Valle
  • Helberg Asencio-Santofimio Pontificia Universidad Javeriana, Cali
  • Jose Oscar Gutierrez-Montes Universidad del Valle
  • Angelica García Pontificia Universidad Javeriana, Cali.
  • Silverio Garzón-Gaviria Pontificia Universidad Javeriana, Cali.



Zanthoxylum rhoifolium, nociception, neuromotor effect, rotarod test, non-polar extract, formalin test


BACKGROUND: Colombia has a high diversity of medicinal plants, including Tachuelo (Zanthoxylum rhoifolium), a tree belonging to the family Rutaceae, which has been attributed an analgesic use by communities of the Colombian southwest. Nevertheless, this species has been scarce studied in Colombia. The studies have been limited to the isolation of compounds with antifungal and insecticide activity, using the leaves, branches, and bark of the plant in aqueous, and organic extracts. Neither pharmacological tests have been carried out, nor the analgesic and neuromotor activity have been tested from preparations with this plant’s fruits. OBJECTIVES: To evaluate the analgesic and neuromotor effect of the aqueous and hexane extract of the fruits of Z. rhoifolium. METHODS: As there were no preliminary reports of this study, the first observation was made through Irwin’s test. The formalin and the rotarod test were performed to determine the analgesic and motor coordination effects, respectively. RESULTS: All the evaluated treatments demonstrated to generate analgesia, anesthesia, passivity, reduction in the alarm reaction, and have antinociceptive activity in the formalin test; while only the high dose of the nonpolar extract generated a deficit in the motor performance of the rodents in the rotarod test. CONCLUSIONS: the antinociceptive effect of the aqueous and hexane extracts of this species’ fruits was demonstrated. The hexane extract generated a neuromotor effect, which validates the ethnobotanical reports on Z. rhoifolium fruits.

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Author Biographies

Melany Villegas-Valencia, Pontificia Universidad Javeriana.

Visiting Student in the Red Sea Research Center at the Biological Oceanography Laboratory

Libardo Andrés González-Reyes, Universidad del Valle

Master's student in Biosciences at the Universidad del Valle and professor at the Pontificia Universidad Javeriana, Cali and Icesi.

Helberg Asencio-Santofimio, Pontificia Universidad Javeriana, Cali

Director of the Department of Basic Health Sciences.

Jose Oscar Gutierrez-Montes, Universidad del Valle

Associate Professor at the Faculty of Health, School of Basic Sciences, Department of Physiology.

Angelica García, Pontificia Universidad Javeriana, Cali.

Professor associated with the Department of Basic Health Sciences.

Silverio Garzón-Gaviria, Pontificia Universidad Javeriana, Cali.

Associate Professor in the Department of Basic Health Sciences


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How to Cite

Villegas-Valencia, M., González-Reyes, L. A., Asencio-Santofimio, H., Gutierrez-Montes, J. O., García, A., & Garzón-Gaviria, S. (2020). Analgesic and neuromotor effects of two extracts of Zanthoxylum rhoifolium (Rutaceae) in the Swiss albino mouse (Mus musculus). Vitae, 27(3).



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