High polarity extracts obtained from Ilex guayusa Loes. leaves, exhibit antioxidant capacity

Authors

DOI:

https://doi.org/10.17533/udea.vitae.v29n1a347297

Keywords:

Ilex guayusa Loes, Antioxidant capacity, DPPH free radical scavenging (FRS), ABTS, Phenolic compounds

Abstract

Background: Ilex guayusa Loes. belongs to the family Aquifoliaceae. It is an evergreen tree native to the Amazon region. According to traditional uses, it is used as a diaphoretic, narcotic, purgative, among other uses.
 
Objective: To evaluate the antioxidant capacity, quantify phenols and total flavonoids of extracts obtained from Ilex guayusa leaves.
 
Methods: Total ethanolic extract was obtained using Soxhlet-type equipment, with subsequent liquid/liquid fractionation with solvents of different polarities (petroleum ether, dichloromethane, and ethyl acetate); were reacted with 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS•+), determining the inhibitory concentration 50 (IC50) and relative antioxidant activity (%AAR), with subsequent statistical analysis (ANOVA and Tukey's post-hoc). Total phenols and flavonoids were quantified using the Folin-Ciocalteu method and complex formation with AlCl3, respectively.
 
Results: The ethanolic extract of leaves showed the highest antioxidant activity in both the DPPH• and ABTS•+ methods with IC50 values of 4.58 and 3.82 ppm, respectively.
 
Conclusions: According to the results obtained in this study, it was possible to corroborate that the ethanolic extract (EE) obtained from the leaves of I. guayusa showed the highest antioxidant capacity, by the DPPH• and ABTS•+ methods. Additionally, it was possible to relate this capacity to the high flavonoids content present in this extract. Based on the above and accompanied by future studies, the Ilex guayusa species could be proposed as an important source of antioxidant compounds with possible application in medicine and the food industry.
|Abstract
= 1177 veces | PDF
= 533 veces| | HTML
= 2 veces|

Downloads

Download data is not yet available.

Author Biographies

OSCAR EDUARDO RODRIGUEZ AGUIRRE, Universidad El Bosque, Engineering Faculty, Research Group CHOC-IZONE, Bogotá, Colombia

Lic. Química y Biología, magister en Bióloga, Doctor en ciencias Biológicas

Jenny Carolina Castañeda Villate, Fundación Universitaria Juan N. Corpas, GIFVTA Research Group, Bogotá Colombia

Médica, especialista en terapéuticas alternativas y farmacología vegetal

Mayerly Johanna Hernández Roa, Fundación Universitaria Juan N. Corpas, GIFVTA Research Group, Bogotá Colombia

Médica, especialista en terapéuticas alternativas y farmacología vegetal

Camila Florenza Cárdenas Tavera, Fundación Universitaria Juan N. Corpas, GIFVTA Research Group, Bogotá Colombia

Médica, especialista en terapéuticas alternativas y farmacología vegetal

Luis Miguel Pombo Ospina, Fundación Universitaria Juan N. Corpas, GIFVTA Research Group, Bogotá Colombia

Ingeniero quimico, Magister en Ciencias Biologicas

Paola Borrego Muñoz, Fundación Universitaria Juan N. Corpas, GIFVTA Research Group, Bogotá Colombia

Docente Investigador e Integrante Grupo de investigación en Farmacología Vegetal y Terapéuticas Alternativas

References

Lewis WH, Kennelly EJ, Bass GN, Wedner HJ, Elvin-Lewis MP, Fast D. Ritualistic use of the holly Ilex guayusa by Amazonian Jivaro Indians. J Ethnopharmacol .1991;33:25–30.

Arteaga-Crespo Y, Radice M, Bravo-Sanchez LR, García-Quintana Y, Scalvenzi L. Optimisation of ultrasound-assisted extraction of phenolic antioxidants from Ilex guayusa Loes. leaves using response surface methodology. Heliyon .2020;6:0–7. https://doi.org/10.1016/j.heliyon.2019.e03043.

Schultes RE. Fifteen years of study of psychoactive snuffs of south america: 1967-1982- a review*. J Ethnopharmacol .1984;11:17–32.

Dickau R, Redwood SD, Cooke RG. A 4,000-year-old shaman’s stone cache at Casita de Piedra, western Panama. Archaeol Anthropol Sci .2013;5:331–49. https://doi.org/10.1007/s12520-012-0112-5.

Cadena-Carrera S, Tramontin DP, Bella Cruz A, Bella Cruz RC, Müller JM, Hense H. Biological activity of extracts from guayusa leaves (Ilex guayusa Loes.) obtained by supercritical CO2 and ethanol as cosolvent. J Supercrit Fluids .2019;152:104543. https://doi.org/10.1016/j.supflu.2019.104543.

Chóez-Guaranda I, Viteri-Espinoza R, Barragán-Lucas A, Manzano P, Viteri-Espinoza R, Barragán-Lucas A. Effect of solvent-solvent partition on antioxidant activity and GC-MS profile of Ilex guayusa Loes . leaves extract and fractions. Nat Prod Res .2021;Feb 4:1–5. https://doi.org/10.1080/14786419.2021.1882455.

García-Ruiz A, Baenas N, Benítez-Gonzáñez AM, Stinco C, Meléndez-Martínez A, Moreno D, et al. Guayusa (Ilex guayusa L.) new tea: phenolic and carotenoid composition and antioxidant capacity. J Sci Food Agric .2017;97:3929–36. https://doi.org/10.1002/jsfa.8255.

Villacís-Chiriboga J, García-Ruiz A, Baenas N, Moreno DA, Meléndez-Nartínez A, Stinco C, et al. Changes in phytochemical composition, bioactivity and in vitro digestibility of Guayusa leaves (Ilex guayusa Loes.) in different ripening stages. J Sci Food Agric .2018;98:1927–34. https://doi.org/10.1002/jsfa.8675.

Pardau MD, Pereira ASP, Serem JC, Bester MJ. Antioxidant and anti-inflammatory properties of Ilex guayusa tea preparations: a comparison to Camellia sinensis teas. Food Funct .2019;8:4601–10. https://doi.org/10.1039/c7fo01067b.

Fahmy NM, Al-Sayed E, El-Shazly M, Singab AN. Comprehensive review on flavonoids biological activities of Erythrina plant species. Ind Crops Prod .2018;123:500–38. https://doi.org/10.1016/j.indcrop.2018.06.028.

Janabi AHW, Kamboh AA, Saeed M, Xiaoyu L, BiBi J, Majeed F, et al. Flavonoid-rich foods (FRF): A promising nutraceutical approach against lifespan-shortening diseases. Iran J Basic Med Sci .2020;23:140–53. https://doi.org/10.22038/IJBMS.2019.35125.8353.

Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT - Food Sci Technol .1995;28:25–30. https://doi.org/10.1016/S0023-6438(95)80008-5.

Folin O, Ciocalteau V. Tyrosine and Tryptophane in Proteins. J Biol Chem .1927;73:627–48.

Ainsworth EA, Gillespie KM. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent. Nat Protoc .2007;2:875–7. https://doi.org/10.1038/nprot.2007.102.

Wolfe K, Wu X, Liu RH. Antioxidant activity of apple peels. J Agric Food Chem .2003;51:609–14. https://doi.org/10.1021/jf020782a.

Pękal A, Pyrzynska K. Evaluation of Aluminium Complexation Reaction for Flavonoid Content Assay. Food Anal Methods .2014;7:1776–82. https://doi.org/10.1007/s12161-014-9814-x.

Palomino, Lady, García C, Gil J, Rojano B, Durango D. Determination of phenolic content and evaluation of actioxidant activity of propolis from Antioquia (Colombia). Vitae, Rev La Fac Química Farm .2009;16:388–95.

Marquele FD, Di Mambro VM, Georgetti SR, Casagrande R, Valim YML, Fonseca MJ V. Assessment of the antioxidant activities of Brazilian extracts of propolis alone and in topical pharmaceutical formulations. J Pharm Biomed Anal .2005;39:455–62. https://doi.org/10.1016/j.jpba.2005.04.004.

Jara A, Rodriguez Y, Cornejo J, Cazar M, Gutierrez M, Astudillo L. Antioxidant activity and total phenolics of plants used in traditional medicine in Ecuador, .2013, p. 1–6. https://doi.org/10.3390/ecsoc-17-b001.

Pietta PG. Flavonoids as Antioxidants. J Nat Prod .2000;63:1035–42. https://doi.org/10.1021/np9904509.

Wise G, Negrin A. A critical review of the composition and history of safe use of guayusa: a stimulant and antioxidant novel food. Crit Rev Food Sci Nutr .2019;60:2393–404. https://doi.org/10.1080/10408398.2019.1643286.

DPPH

Downloads

Published

07-02-2022

How to Cite

RODRIGUEZ AGUIRRE, O. E., Castañeda Villate, J. C., Hernández Roa, M. J. ., Cárdenas Tavera, C. F. ., Pombo Ospina, L. M. . ., & Borrego Muñoz, P. . (2022). High polarity extracts obtained from Ilex guayusa Loes. leaves, exhibit antioxidant capacity. Vitae, 29(1). https://doi.org/10.17533/udea.vitae.v29n1a347297

Issue

Vol. 29 No. 1 (2022)

Section

Natural Products

License

Copyright (c) 2022 OSCAR EDUARDO RODRIGUEZ AGUIRRE, Jenny Carolina Castañeda Villate, Mayerly Johanna Hernández Roa, Camila Florenza Cárdenas Tavera, Luis Miguel Pombo Ospina, Paola Borrego Muñoz

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Copyright Notice and Open Access Statement

The Journal Vitae works under the Open Access license, and the published manuscripts remain available for the public, both on the Journal's website and in databases, under the Creative Commons license, "Noncommercial Attribution" and "Share alike" systems, adopted in Colombia. Hence, when the authors agree to publish in the Journal Vitae, they will not have the right to economic retributions on publications and reproductions through different diffusion media. The documents are freely available to the internet public, permitting users to read, download, copy, distribute, print, search, or link to the full texts and pass them as data to software. The only constraint on reproduction and distribution, should be to give authors control over the integrity of their work and the right to be appropriately acknowledged and cited.

Authors declare that:

  1. They are the intellectual property owners and are responsible for all the information stated in the article.

  2. This manuscript has not been submitted or published in other printed or digital media. They accept the responsibility for the judgments, opinions, and points of view expressed in the published article and, therefore, they exonerate Universidad de Antioquia and Journal Vitae from any process.

  3. They exempt Universidad de Antioquia and Journal Vitae from settling conflicts or disputes related to the authorship of the referred article.

  4. They accept the revision of the original manuscript by suitable personnel, and they bind themselves to perform the corrections appointed or suggested by the assessors.

  5. Therefore, they know the editorial process and will not bind the Editorial Board of the Journal to assume any obligations regarding the volume and issue in which the article is published.

  6. They transfer the rights of publication, reprinting, and distribution of the article from the moment of its approval, in print and digital format, without the right to economic rewards, and under the licensing conditions considered relevant by Journal Vitae.

  7. They fully authorize Universidad de Antioquia and Journal Vitae to submit the published material to the diverse databases and indexing systems where the Journal can be found to comply with the requirements of the regulatory authorities to maintain the national classification of journals.

  8. They will assume the article publication costs established for the current issue, and they will make the payment as soon as they are informed about the volume and the issue in which the final version of the article is published.

  9. After the article is published, you can share digital or printed copies in a noncommercial manner. You will be able to use the paper in your institution or company for educational or research purposes, including the use in course programs.

Conflict of interest: Authors are responsible for recognizing and disclosing any financial or other benefits that could be perceived to bias their work, acknowledging all financial support and any personal connections with potential sponsors. Examples of such conflicts include receiving research funds or honoraria, serving on advisory boards, stock ownership, or employment and consulting arrangements. Authors without such connections should clearly state that they have no financial support or personal relationships that could be perceived to bias their work. All conflicts of interest should be disclosed on the author's identification page of the manuscript.

Crossref
Scopus
Google Scholar
Europe PMC