High polarity extracts obtained from Ilex guayusa Loes. leaves, exhibit antioxidant capacity

Authors

DOI:

https://doi.org/10.17533/udea.vitae.v29n1a347297

Keywords:

Ilex guayusa Loes, Antioxidant capacity, DPPH free radical scavenging (FRS), ABTS, Phenolic compounds

Abstract

Background: Ilex guayusa Loes. belongs to the family Aquifoliaceae. It is an evergreen tree native to the Amazon region. According to traditional uses, it is used as a diaphoretic, narcotic, purgative, among other uses.
 
Objective: To evaluate the antioxidant capacity, quantify phenols and total flavonoids of extracts obtained from Ilex guayusa leaves.
 
Methods: Total ethanolic extract was obtained using Soxhlet-type equipment, with subsequent liquid/liquid fractionation with solvents of different polarities (petroleum ether, dichloromethane, and ethyl acetate); were reacted with 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS•+), determining the inhibitory concentration 50 (IC50) and relative antioxidant activity (%AAR), with subsequent statistical analysis (ANOVA and Tukey's post-hoc). Total phenols and flavonoids were quantified using the Folin-Ciocalteu method and complex formation with AlCl3, respectively.
 
Results: The ethanolic extract of leaves showed the highest antioxidant activity in both the DPPH and ABTS•+ methods with IC50 values of 4.58 and 3.82 ppm, respectively.
 
Conclusions: According to the results obtained in this study, it was possible to corroborate that the ethanolic extract (EE) obtained from the leaves of I. guayusa showed the highest antioxidant capacity, by the DPPH and ABTS•+ methods. Additionally, it was possible to relate this capacity to the high flavonoids content present in this extract. Based on the above and accompanied by future studies, the Ilex guayusa species could be proposed as an important source of antioxidant compounds with possible application in medicine and the food industry.
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Author Biographies

OSCAR EDUARDO RODRIGUEZ AGUIRRE, Universidad El Bosque, Engineering Faculty, Research Group CHOC-IZONE, Bogotá, Colombia

Lic. Química y Biología, magister en Bióloga, Doctor en ciencias Biológicas

Jenny Carolina Castañeda Villate, Fundación Universitaria Juan N. Corpas, GIFVTA Research Group, Bogotá Colombia

Médica, especialista en terapéuticas alternativas y farmacología vegetal

Mayerly Johanna Hernández Roa, Fundación Universitaria Juan N. Corpas, GIFVTA Research Group, Bogotá Colombia

Médica, especialista en terapéuticas alternativas y farmacología vegetal

Camila Florenza Cárdenas Tavera, Fundación Universitaria Juan N. Corpas, GIFVTA Research Group, Bogotá Colombia

Médica, especialista en terapéuticas alternativas y farmacología vegetal

Luis Miguel Pombo Ospina, Fundación Universitaria Juan N. Corpas, GIFVTA Research Group, Bogotá Colombia

Ingeniero quimico, Magister en Ciencias Biologicas

Paola Borrego Muñoz, Fundación Universitaria Juan N. Corpas, GIFVTA Research Group, Bogotá Colombia

Docente Investigador e Integrante Grupo de investigación en Farmacología Vegetal y Terapéuticas Alternativas

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Published

07-02-2022

How to Cite

RODRIGUEZ AGUIRRE, O. E., Castañeda Villate, J. C., Hernández Roa, M. J. ., Cárdenas Tavera, C. F. ., Pombo Ospina, L. M. . ., & Borrego Muñoz, P. . (2022). High polarity extracts obtained from Ilex guayusa Loes. leaves, exhibit antioxidant capacity. Vitae, 29(1). https://doi.org/10.17533/udea.vitae.v29n1a347297

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Natural Products

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