Evaluation of Cytotoxic effect of Moringa peregrina seeds on Oral Cancer, CAL 27 Cell Line and Red Blood Cells Hemolysis

Authors

DOI:

https://doi.org/10.17533/udea.vitae.v31n1a350974

Keywords:

CAL 27 cell line, Moringa peregrina seed extract, Erythrocyte hemolysis, Toxicity, Anticancer

Abstract


Background: Moringa peregrina Forssk is a well-known plant in ethnomedicine due to its widespread uses in various diseases like cough, wound healing, rhinitis, fever, and detoxification. The plant seeds
contain compounds that are cytotoxic to many cancer cells. During the therapeutic use of plants via the oral route, some compounds present in the plants may be cytotoxic to normal cell lines and red blood cells.
Objective: This study was the first report of investigation of the cytotoxic profile on oral cancer, CAL 27, cell line, and hemolytic activities on human erythrocytes of Moringa peregrina seeds ethanolic extract (MPSE).
Methods: MPSE was screened for its cytotoxic effect against oral cancer, CAL 27, cell line using 3-(4, 5-dimethylthiazol-2-yl)-2, 5,-diphenyltetrazolium bromide (MTT) assay. The toxicity of MPSE on human erythrocytes was determined by in vitro hemolytic assay.
Results: MPSE showed significant anti-proliferative activity against oral cancer, CAL 27 cell line at lower concentrations with half maximal inhibitory concentration (IC50) value of 21.03 μg/mL. At 1,000 µg/ml of MPSE, the maximum hemolysis was found to be 14.3% which is within safer limit.
Conclusions: This study revealed a potential anti-oral cancer of MPSE and provided a baseline for its potential use in oral cancer treatment with minimum hemolytic effect on human RBCs.
|Abstract
= 381 veces | PDF
= 408 veces| | HTML
= 3 veces|

Downloads

Download data is not yet available.

References

- Abid AM, Merza MS. Immunohistochemical expression of Cyclin D1 and NF-KB p65 in oral lichen planus and oral squamous cell carcinoma (Comparative study). J Baghdad Coll Dent. 2014;26:80-7. https://jbcd.uobaghdad.edu.iq/index.php/jbcd/article/view/301.

- Tsai LL, Yu CC, Chang YC, Yu CH, Chou MY. Markedly increased Oct4 and Nanog expression correlates with cisplatin resistance in oral squamous cell carcinoma. J. Oral Pathol Med. 201;40(8): 621-628. DOI: https://doi.org/10.1111/j.1600-0714.2011.01015.x

- Fuoad SAA, Mohammad DN, Hamied MAS, Garib BT. Oro-facial malignancy in north of Iraq: a retrospective study of biopsied cases. BMC Oral Health. 2021;21(1):1-10. DOI: https://doi.org/10.1186/s12903-021-01521-3.

- Usman S, Jamal A, Teh MT, Waseem A. Major Molecular Signaling Pathways in Oral Cancer Associated with Therapeutic Resistance. Front Oral Health. 2021;1. DOI: https://doi.org/10.3389/froh.2020.603160.

- Ali M, Abbas A, Drea A. Green synthesis of nano binary oxide SiO2/V2O5 NPs integrated ointment cream application on wound dressings and skin cancer cells. Baghdad Sci J. 2022;20(3):0734. DOI: https://doi.org/10.21123/bsj.2022.7318.

- Nazhvani AD, Sarafraz N, Askari F, Heidari F, Razmkhah M.. Anti-cancer effects of traditional medicinal herbs on oral squamous cell carcinoma. Asian Pac J Cancer Prev. 2020;21(2):479. DOI: https://doi.org/10.31557/APJCP.2020.21.2.479.

- Cardona-Mendoza A, Olivares-Niño G, Díaz-Báez D, Lafaurie GI, Perdomo SJ. Chemopreventive and Anti-tumor Potential of Natural Products in Oral Cancer. Nutr Cancer. 2022;74(3):779-795. DOI: https://doi.org/10.1080/01635581.2021.1931698.

- Al-Bahrani RM, Radif HM, Albaayit SFA. Evaluation of potent silver nanoparticles production from Agaricus bisporus against Helicobacter pylori.

Pak J Agric Sci. 2020;57(4):1197-1201. DOI: https://doi.org/10.21162/PAKJAS/20.9893.

- Albaayit SFA, Rasedee A, Abdullah N. Zerumbone-loaded nanostructured lipid carrier gel facilitates wound healing in rats. Rev. bras. farmacogn. 2020;30(2):272-278. DOI: https://doi.org/10.1007/s43450-020-00023-7.

- Albaayit SFA, Rasedee A, Abdullah N, Abba Y. Methanolic extract of Clausena excavata promotes wound healing via antiinflammatory and anti-apoptotic activities. Asian PacJ Trop Biomed. 2020;10(5):232-238. DOI: https://doi.org/10.4103/2221-1691.281467.

- Albaayit SFA. In vitro evaluation of anticancer activity of Moringa peregrina seeds on breast cancer cells. Eurasia J Math Sci Technol Educ. 2020;11:163-166. http://www.epstem.net/en/download/article-file/1439710.

- Albaayit SFA, Maharjan R, Khan M. Evaluation of hemolysis activity of Zerumbone on RBCs and brine shrimp toxicity. Baghdad Sci J. 2021;18(1):65-69. DOI: https://doi.org/10.21123/bsj.2021.18.1.0065.

- Albaayit SFA, Ozaslan M. Cytotoxic and urease inhibition potential of Moringa peregrina seed ethanolic extract. Int J Pharm. 2019; 15(1): 151-155. DOI: https://doi.org/10.3923/ijp.2019.151.155.

- Abd Rani NZ, Husain K, Kumolosasi E. Moringa genus: a review of phytochemistry and pharmacology. Front Pharmacol. 2018;9:108. DOI: https://doi.org/10.3389/fphar.2018.00108.

- Albaayit SFA, Al-Khafaji ASK, Alnaimy HS. In vitro macrophage nitric oxide and interleukin-1 beta suppression by Moringa peregrina seed. Turk J Pharm Sci. 2019; 16(3): 362-365. DOI: https://doi.org/10.4274/tjps.galenos.2018.52244.

- Soltan MM, Zaki AK. Antiviral screening of forty-two Egyptian medicinal plants. J ethnopharmacol. 2009;126(1):102-107. DOI: https://doi.org/10.1016/j.jep.2009.08.001.

- Albaayit SFA, Maharjan R. Immunomodulation of Zerumbone via Decreasing the Production of Reactive Oxygen Species from Immune Cells. Pak J Biol Sci. 2018;21(9):475-479. DOI: https://doi.org/10.3923/pjbs.2018.475.479.

Albaayit SFA. Enzyme inhibitory properties of zerumbone. Pak J Agri Sci. 2021;58(3):1207-1209. DOI: https://doi.org/10.21162/PAKJAS/21.9759.

Albaayit SFA. Evaluation of anti-methicillin resistant Staphylococcus aureus property of Clausena excavata leaves by using atomic force microscopy and flowcytometry techniques. Pak J Agri Sci. 2021;58(1):315-320. DOI: https://doi.org/10.3389/froh.2020.603160.

Albaayit SFA, Maharjan R, Abdullah R, Noor MHM. Anti-Enterococcus Faecalis, Cytotoxicity, Phytotoxicity, and Anticancer Studies on Clausena excavataBurum. f. (Rutaceae) Leaves. BioMed Res Int. 2021. DOI: https://doi.org/10.1155/2021/3123476.

Albaayit SFA, Mohammed MK. Antiangiogenic activity and ROS-mediated lung cancer cell line injury of zerumbone. J Fac Pharm Ankara. 2023;47(3). DOI: https://doi.org/10.33483/jfpau.1112778.

Wiradharma N, Khoe U, Hauser CA, Seow SV, Zhang S, Yang YY. Synthetic cationic amphiphilic α-helical peptides as antimicrobial agents. Biomaterials. 2011;32(8):2204-2212. DOI: https://doi.org/10.1016/j.biomaterials.2010.11.054.

Fang L, Gao L, Xie L, Xiao G. Eukaryotic translation initiation factor 5A-2 involves in doxorubicin-induced epithelial-mesenchymal transition in oral squamous cell carcinoma cells. J Cancer. 2018;9(19):3479-3488. DOI: https://doi.org/10.7150/jca.26136.

Sadraei H, Asghari G, Farahnaki F. Assessment of hydroalcoholic extract of seeds and leaves of Moringa peregrina on ileum spasm. Res Pharm Sci. 2015;10(3):252-258. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4621632/pdf/RPS-10-252.pdf

Abou-Hashem MM, Abo-Elmatty DM, Mesbah NM, Abd EL-Mawgoud AM. Induction of sub-G0 arrest and apoptosis by seed extract of Moringa peregrina (Forssk.) Fiori in cervical and prostate cancer cell lines. J Integr Med. 2019;17(6):410-422. DOI: https://doi.org/10.1016/j.joim.2019.09.004.

Abaza MS, Al-Attiyah RA, Bhardwaj R, Abbadi G, Koyippally M, Afzal M. Syringic acid from Tamarix aucheriana possesses antimitogenic and chemo-sensitizing activities in human colorectal cancer cells. Pharm. Biol. 2013;51(9):1110-1124. DOI: https://doi.org/10.3109/13880209.2013.781194.

Jaganathan SK, Supriyanto E, Mandal M. Events associated with apoptotic effect of p-Coumaric acid in HCT-15 colon cancer cells. World J Gastroenterol.2013;19(43):7726-7734. DOI: https://doi.org/103748/wjg.v19.i43.7726.

Maiyoa F, Moodley R, Singh M. Phytochemistry, cytotoxicity and apoptosis studies of β-sitosterol-3-oglucoside and β-amyrin from Prunus africana. African Journal of Traditional. BMC Complement Altern Med. 2016;13(4):105-112. DOI: https://doi.org/10.21010/ajtcam.v13i4.15.

Bao L, Liu F, Guo HB, Li Y, Tan BB, Zhang WX, Peng YH. Naringenin inhibits proliferation, migration, and invasion as well as induces apoptosis of gastric cancer SGC7901 cell line by downregulation of AKT pathway. Tumour Biol. 2016;37:11365-11374. DOI: https://doi.org/10.1007/s13277-016-5013-2.

Albaayit SFA, Khan M, Abdullah R, Noor MHM. Ethyl acetate extract of Clausena excavata induces growth inhibition of non-small-lung cancer, NCI-H460, cell line via apoptosis. J Appl Biomed. 2021;19(1):40-47. DOI: https://doi.org/10.32725/jab.2021.007

Wang T, Gong X, Jiang R, Li H, Du W, Kuang G. Ferulic acid inhibits proliferation and promotes apoptosis via blockage of PI3K/Akt pathway in osteosarcoma cell. Am J Transl Res.2016;8(2):968-980. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4846940.

Hua F, Li CH, Chen XG, Liu XP. Daidzein exerts anticancer activity towards SKOV3 human ovarian cancer cells by inducing apoptosis and cell cycle arrest, and inhibiting the Raf/MEK/ERK cascade. Int J mol med. 2018;41(6): 3485-3492. DOI: https://doi.org/10.3892/ijmm.2018.3531.

Niero EL, Machado-Santelli GM. Cinnamic acid induces apoptotic cell death and cytoskeleton disruption in human melanoma cells. J Exp Clin. Cancer Res. 2013;32(1):1-14. DOI: https://doi.org/10.1186/1756-9966-32-31.

Hashemzaei M, Delarami Far A, Yari A, Heravi RE, Tabrizian K, Taghdisi SM, Sadegh SE, Tsarouhas K, Kouretas D, Tzanakakis G, Nikitovic D. Anticancer and apoptosis‑inducing effects of quercetin in vitro and in vivo. Oncol Repo. 2017;38(2): 819-828. DOI: https://doi.org/10.3892/or.2017.5766.

Moringa peregrina Forssk (https://powo.science.kew.org/taxon/urn:lsid:ipni.org:names:584740-1)

Downloads

Published

05-03-2024

How to Cite

Shaymaa, & Maharjan, R. (2024). Evaluation of Cytotoxic effect of Moringa peregrina seeds on Oral Cancer, CAL 27 Cell Line and Red Blood Cells Hemolysis. Vitae, 31(1). https://doi.org/10.17533/udea.vitae.v31n1a350974

Issue

Vol. 31 No. 1 (2024)

Section

Natural Products

License

Copyright (c) 2024 Shaymaa, Rukesh Maharjan

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Copyright Notice and Open Access Statement

The Journal Vitae works under the Open Access license, and the published manuscripts remain available for the public, both on the Journal's website and in databases, under the Creative Commons license, "Noncommercial Attribution" and "Share alike" systems, adopted in Colombia. Hence, when the authors agree to publish in the Journal Vitae, they will not have the right to economic retributions on publications and reproductions through different diffusion media. The documents are freely available to the internet public, permitting users to read, download, copy, distribute, print, search, or link to the full texts and pass them as data to software. The only constraint on reproduction and distribution, should be to give authors control over the integrity of their work and the right to be appropriately acknowledged and cited.

Authors declare that:

  1. They are the intellectual property owners and are responsible for all the information stated in the article.

  2. This manuscript has not been submitted or published in other printed or digital media. They accept the responsibility for the judgments, opinions, and points of view expressed in the published article and, therefore, they exonerate Universidad de Antioquia and Journal Vitae from any process.

  3. They exempt Universidad de Antioquia and Journal Vitae from settling conflicts or disputes related to the authorship of the referred article.

  4. They accept the revision of the original manuscript by suitable personnel, and they bind themselves to perform the corrections appointed or suggested by the assessors.

  5. Therefore, they know the editorial process and will not bind the Editorial Board of the Journal to assume any obligations regarding the volume and issue in which the article is published.

  6. They transfer the rights of publication, reprinting, and distribution of the article from the moment of its approval, in print and digital format, without the right to economic rewards, and under the licensing conditions considered relevant by Journal Vitae.

  7. They fully authorize Universidad de Antioquia and Journal Vitae to submit the published material to the diverse databases and indexing systems where the Journal can be found to comply with the requirements of the regulatory authorities to maintain the national classification of journals.

  8. They will assume the article publication costs established for the current issue, and they will make the payment as soon as they are informed about the volume and the issue in which the final version of the article is published.

  9. After the article is published, you can share digital or printed copies in a noncommercial manner. You will be able to use the paper in your institution or company for educational or research purposes, including the use in course programs.

Conflict of interest: Authors are responsible for recognizing and disclosing any financial or other benefits that could be perceived to bias their work, acknowledging all financial support and any personal connections with potential sponsors. Examples of such conflicts include receiving research funds or honoraria, serving on advisory boards, stock ownership, or employment and consulting arrangements. Authors without such connections should clearly state that they have no financial support or personal relationships that could be perceived to bias their work. All conflicts of interest should be disclosed on the author's identification page of the manuscript.

Crossref
Scopus
Google Scholar
Europe PMC