Estudio de medios de cultivo para la síntesis de licopeno a partir de Clavibacter michiganensis sub. michiganensis
DOI:
https://doi.org/10.17533/udea.vitae.435Keywords:
clavibacter, michiganensis, licopeno, medios de cultivo, antioxidante, clavibacter michiganensis, lycopene, culture media, antioxidantAbstract
El licopeno es un carotenoide de elevado poder antioxidante que se obtiene a partir de fuentes naturales especialmente frutas y vegetales; también puede ser producido mediante el cultivo de microorganismos. En el presente artículo se estudia la síntesis de licopeno a partir del cultivo sumergido de Clavibacter michiganensis sub. michiganensis. Se considrera el diseño de medios de cultivo y la separación, cuantificación y caracterización del carotenoide. Con el fin de establecer los niveles más apropiados para los factores de estudio (fuente de carbono, fuente de nitrógeno y sales), se programa un diseño de factor único; para el estudio de optimización se emplea un diseño central compuesto (33), cuya variable respuesta es la concentración de producto. El ajuste estadístico de los resultados experimentales muestra valores de fuente de carbono (glucosa: 7.5 g/L), nitrógeno (peptona: 10 g/L y extracto de levadura: 10 g/L) y sales (cloruro de sodio: 10 g/L y fosfato dipotásico: 1 g/L), como constituyentes del medio de cultivo para lograr la mayor concentración de pigmento (2.85 mg carotenos/mL). El producto obtenido se caracteriza como licopeno a partir de técnicas de Infrarojo y Resonancia Magnética Nuclear de Protones.Downloads
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