INTRALABORATORY VALIDATION OF AN ANALYTICAL METHODOLOGY BY UV/VIS SPECTROMETRY IN ORDER TO EVALUATE THE OCULAR IRRITANT POTENTIAL OF TENSOACTIVES IN RED BLOOD CELLS
Keywords:Sodium dodecylsulphate, surfactants, hemolysis, validation studies, animal testing alternatives
Red blood cell assay (RBC) intralaboratory validation was presented, specifically for linearity, repeatability and intermediate precision. RBC is an in vitro methodology employed for ocular irritation evaluation measuring surfactants adverse effects on cell membranes and proteins and is an alternative for Draize ocular test. Free hemoglobin after red blood cell lysis was determined through spectrometry at 577 nm in order to estimate medium hemolytic concentration (HC50) indicating cell damage by surfactant. Also hemoglobin denaturation induced by high concentrations of surfactants was considered measuring denaturation index (DI) through absorbance ratio at 542 and 577 determinations. HC50 / DI is an indicator of the dodecylsulphate (SDS) irritant potential, surfactant used in order to validate this assay. Parameters previously mentioned were validated and we present an easy and reliable alternative methodology for animal experimentation to assess surfactants ocular irritation for SDS.
How to Cite
Copyright Notice and Open Access Statement
The Journal Vitae works under the Open Access license, and the published manuscripts remain available for the public, both on the Journal's website and in databases, under the Creative Commons license, "Noncommercial Attribution" and "Share alike" systems, adopted in Colombia. Hence, when the authors agree to publish in the Journal Vitae, they will not have the right to economic retributions on publications and reproductions through different diffusion media. The documents are freely available to the internet public, permitting users to read, download, copy, distribute, print, search, or link to the full texts and pass them as data to software. The only constraint on reproduction and distribution, and the only role for copyright in this domain, should be to give authors control over the integrity of their work and the right to be appropriately acknowledged and cited.
Authors declare that:
They are the intellectual property owners and are responsible for all the information stated in the article.
This manuscript has not been submitted or published in other printed or digital media. They accept the responsibility for the judgments, opinions, and points of view expressed in the published article and, therefore, they exonerate Universidad de Antioquia and Journal Vitae from any process.
They exempt Universidad de Antioquia and Journal Vitae from settling conflicts or disputes related to the authorship of the referred article.
They accept the revision of the original manuscript by suitable personnel, and they bind themselves to perform the corrections appointed or suggested by the assessors.
Therefore, they know the editorial process and will not bind the Editorial Board of the Journal to assume any obligations regarding the volume and issue in which the article is published.
They transfer the rights of publication, reprinting, and distribution of the article from the moment of its approval, in print and digital format, without the right to economic rewards, and under the licensing conditions considered relevant by Journal Vitae.
They fully authorize Universidad de Antioquia and Journal Vitae to submit the published material to the diverse databases and indexing systems where the Journal can be found to comply with the requirements of the regulatory authorities to maintain the national classification of journals.
They will assume the article publication costs established for the current issue, and they will make the payment as soon as they are informed about the volume and the issue in which the final version of the article is published.
After the article is published, you can share digital or printed copies in a noncommercial manner. You will be able to use the paper in your institution or company for educational or research purposes, including the use in course programs.
Conflict of interest: Authors are responsible for recognizing and disclosing any financial or other benefits that could be perceived to bias their work, acknowledging all financial support and any personal connections with potential sponsors. Examples of such conflicts include receiving research funds or honoraria, serving on advisory boards, stock ownership, or employment and consulting arrangements. Authors without such connections should clearly state that they have no financial support or personal relationships that could be perceived to bias their work. All conflicts of interest should be disclosed on the author's identification page of the manuscript.