MODIFICACION DEL METODO DE FIELD DILUIDO PARA LA COLORACION DE MUESTRAS DE SANGRE EN EL DIAGNOSTICO DEL PALUDISMO
Abstract
The staining of blood has always been complicated by the great variability offered by methylene blue, from which practically all the dyes used in the staining of blood samples are derived.
The thick drop was initially colored by covering a large thick drop with diluted methylene blue, but as there was almost no visibility in the central part, it was decided to defibrinate the blood to facilitate diagnosis.
Pampana's discovery of the dehemoglobinizing property of the isotonic salts of methylene blue contributed to obtaining better staining by preceding them with a pretreatment with a solution of phosphate methylene blue.
By means of this previous step, stains are obtained that offer clean fields for microscopic observation, facilitating the identification of blood elements and parasites.
Field's method of rapid staining for thick drops consists of a 1 to 3 second dip in solution “A” (a mixture of methylene blue and phosphates), followed by a brief rinse in distilled water and a similar dip in solution “B” (a mixture of eosin and phosphates).
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References
Boyd, Mark Frederick. Malariology. Philadelphia, Saunders, 1949.
Field, John W. The microscopic diagnosis of human malaria; Part 1; a short descriptive atlas of thick film diagnosis.
Kuala Lumpur, Govt. Press, 1948. p.116.
"A simple and rapid method of staining malarial parasites in thick blood smears". Trans Roy Soc Trop Med Hyg 34:195-202, 1940.
A simple method of preserving the outline of leukocytes malarial parasites in Giemsa stained thick blood films". Trans Roy Soc Trop Med Hyg 33:625–638, 1940.
and A.A. Sandosham. "The Romanowsky stains aqueous or methanolic" Trans Roy Soc Trop Med Hyg 58:164-172, 1964.
López Antaño, F. J. Coloración de field diluido, 1970 (Documento de trabajo inédito).
Shute, Perey G. and Maryon E. Maryori. Laboratory technique for the study of malaria. London, Churchill,
Singh, J. and J.S.B. Stain. "A review" Indian J. Malarial 10(2):117-129, 1956.
Misra, J.B. and J.S.B. Stain. "Simplified method of preparation" Indian J. Malariol 10(2):115-116,1956.
Singh, J. et. al. "Its preparation in the powder form and the staining technique" Indian J Malariol. 7(3):267-270, 1953.
Walker, A. J. Manual para el diagnóstico microscópico de la malaria. 2ed. s.l., s.e., 1965. p. 49-51.
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