Stimulation of TLRs, Nod-like receptors and Dectin-1 in neutrophils induces the production of proinflammatory cytokines

Abstract

Objective. To evaluate the expression/function of pattern recognition receptors such as Toll-like receptors, RIG-I/MDA5, NOD-like receptors, Dectin-1 and adaptor proteins, in human neutrophils. Basic procedures. We performed a descriptive observational study to assess in human neutrophils purified from peripheral blood, the expression and functionality of genes encoding pattern recognition receptors (PRRs), measured by mRNA production and secretion of pro-inflammatory cytokines, respectively. Findings. We observed that neutrophils express diverse PRRs and adaptor molecules. The stimulation of TLR4, TLR5 and TLR7/8 induce the production of IL-1β and IL-6 and the activation of Dectin-1 leads the secretion of high levels of TNF-α, but low levels of IL-1β and IL-6. Conclusion. Neutrophils express a large number of PRRs and their activation leads to the expression of proinflammatory cytokines.