Immunoenzymatic determination of IgG anti bothrops atrox levels after snake bites

Authors

  • Sadoh Molina Universidad de Antioquia
  • Luz E. Posada Universidad de Antioquia
  • Rafael Otero Patiño Universidad de Antioquia
  • John J. Estrada Universidad de Antioquia

DOI:

https://doi.org/10.17533/udea.iatreia.3505

Keywords:

Snake bites, Bothropsatrox

Abstract

We developed an immunization method for the production of rabbit antisera against Bothrops atroxvenoms. An enzyme-Ilnked assay (ELISA) was standardized in order to measure IgG levels after snake bites. The immune response of rabbits, as determined by Ouchterlony and immunoelectrophoresis techniques, revealed bands of precipitation from the sixtieth day on. Individual variability in the immune response of rabbits was demonstrated. For the measurement of IgG levels In Indians from the Department of Choco (Colombia), ELISA proved to be a sensitive (83.3%) and simple but not an specific procedure, since there were cross-reactions in those previously bitten by B. nasutus. ELISA was also simple and sensitive (100%) for the determination of equine anti B. atrox IgG antibodies in patients treated with antivenom

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Author Biographies

Sadoh Molina, Universidad de Antioquia

Profesor Titular, Facultad de Medicina Veterinaria y Zootecnia. Universidad de Antioquia

Luz E. Posada, Universidad de Antioquia

Profesora Titular, Sección de Inmunóloga, Departamento de Microbiología y Parasitología, Facultad de Medicina.

Rafael Otero Patiño, Universidad de Antioquia

Profesor Titular, Departamento de Pediatría, Facultad de Medicina, Director del Proyecto de Ofídismo en Antioquia y Chocó.

John J. Estrada, Universidad de Antioquia

Profesor Asociado, Sección de Inmunología, Departamento de Microbiología y Parasitología, Facultad de Medicina. Todos de la Universidad de Antioquia, Medellín, Colombia.

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Published

1993-01-20

How to Cite

1.
Molina S, Posada LE, Otero Patiño R, Estrada JJ. Immunoenzymatic determination of IgG anti bothrops atrox levels after snake bites. Iatreia [Internet]. 1993 Jan. 20 [cited 2025 Dec. 5];6(1):pág. 7-12. Available from: https://revistas.udea.edu.co/index.php/iatreia/article/view/3505

Issue

Vol 6, No 1

Section

Original research

License

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