Chitosan/hydroxyapatite scaffolds for tissue engineering manufacturing method effect comparison


  • Diana Marcela Escobar-Sierra Universidad de Antioquia
  • Johan Martins-Martins University of Franche-Comté
  • Claudia Patricia Ossa-Orozco Universidad de Antioquia



Bone, chitosan, hydroxyapatite in situ, hydroxyapatite in powder, scaffolds chitosan/ hydroxyapatite, tissue engineering


The regeneration of bone is one of the main challenges of modern medicine because many diseases including trauma and tumor can cause bone defects. Tissue engineering (TE) is a promising approach to cure these bone diseases since it allows the reconstruction of tissue by colonization and proliferation of a patient is healthy cells is in an artificial extracellular matrix (scaffolds). The aim of this project was to prepare chitosan/hydroxyapatite CH/HA scaffolds, using various ratios and two different methods: powder hydroxyapatite (commercial) and in situ hydroxyapatite, and then compare their properties. The morphology, chemical composition, and mechanical properties were evaluated by Scanning Electron Microscopy (SEM), X-ray diffraction (XRD), Raman spectroscopy, and compression tests. The scaffolds obtained showed an interconnected porous structure. Scaffolds with better applications in tissue engineering are the scaffolds with chitosan and hydroxyapatite in situ protocol, due to their better morphology; they allow the cell growth. These scaffolds meet the requirements of tissue engineering.



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Author Biographies

Diana Marcela Escobar-Sierra, Universidad de Antioquia

Docente Programa de Bioingeniería, Facultad de Ingeniería

Investigadora Grupo de Investigación en Biomateriales (BIOMAT),

Johan Martins-Martins, University of Franche-Comté

Franche-Comté Higher Institute of Engineering

Claudia Patricia Ossa-Orozco, Universidad de Antioquia

Profesora del Programa de Bioingeniería, Facultad de Ingeniería

Investigadora del Grupo de Investigación en Biomateriales (BIOMAT)


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How to Cite

Escobar-Sierra, D. M., Martins-Martins, J., & Ossa-Orozco, C. P. (2015). Chitosan/hydroxyapatite scaffolds for tissue engineering manufacturing method effect comparison. Revista Facultad De Ingeniería Universidad De Antioquia, (75), 24–35.