Expression of type III collagen in hypertrophic gingival tissue of patients with orthodontic treatment: a pilot study

Authors

DOI:

https://doi.org/10.17533/udea.rfo.v32n2a5

Keywords:

Gingival hypertrophy, Collagen, Immunohistochemistry, Orthodontics, Type III collagen

Abstract


Introduction: gingival hypertrophy (GH) is the uncontrolled increase in gingival volume induced by different etiological factors, including orthodontic treatment. This pathology is characterized by changes in epithelial and connective tissue, including modifications in the extracellular matrix. The present study determined the presence and distribution of type III collagen in tissues of patients with GH wearing fixed orthodontic appliances. Methods: 12 samples of gingival tissue were obtained from patients undergoing periodontal surgery. They were divided into two groups, the first with healthy patients (control; n = 6) and the second with patients diagnosed with GH and orthodontic treatment (patients; n = 6). Each obtained sample was subjected to the hematoxylin-eosin stain, Masson-Goldner staining, and type III collagen immunohistochemistry. Results: the hematoxylin-eosin and Masson-Goldner histological stains showed hypertrophia of epithelial tissue and connective tissue with a marked collagen fiber increase in the gingival tissue of orthodontic wearers with GH compared to individuals in the control group. The gingival tissue of patients with GH caused by orthodontic treatment showed a distribution and location of type III collagen near the basal lamina, around the blood vessels, but unlike the control group, its location was noticeable throughout the connective tissue. Conclusion: the gingival tissues of orthodontic wearers with GH experience an increase in the number and density of collagen fibers. Type III collagen seems to lose its usual location in the gingival tissues of orthodontic wearers with GH.

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Author Biographies

Víctor Hugo Simancas-Escorcia, Universidad de Cartagena

DDS. Specialist in Pedagogy and Teaching. MSc in Cellular Biology, Physiology and Pathology. PhD in Physiology and Pathology, Université de Paris, France. Researcher at the GITOUC group, School of Dentistry, Universidad de Cartagena.

Jocsan Steven Leal-Betancur, Universidad de Cartagena

School of Young Researchers, GITOUC Group, School of Dentistry, Universidad de Cartagena, Colombia.

Antonio Díaz-Caballero, Universidad de Cartagena

DDS. Specialist in Periodontics. MSc in Education. PhD in Biomedical Sciences, Universidad de Cartagena. Head of the GITOUC Research Group. Professor, Universidad de Cartagena

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Published

2020-10-23

How to Cite

Simancas-Escorcia, V. H., Leal-Betancur, J. S., & Díaz-Caballero, A. (2020). Expression of type III collagen in hypertrophic gingival tissue of patients with orthodontic treatment: a pilot study. Revista Facultad De Odontología Universidad De Antioquia, 32(2), 53–63. https://doi.org/10.17533/udea.rfo.v32n2a5