EVALUATION OF PECTIN SOLUBILIZATION CAPABILITY FROM LEMON PEEL USING PROTOPECTINASE-SE

  • Arley D. ZAPATA Z. Facultad de Ciencias, Universidad Nacional de Colombia Sede Medellín.
  • Carlos A. ESCOBAR G. Facultad de Ciencias, Universidad Nacional de Colombia Sede Medellín.
  • Sebastián F. CAVALLITO Centro de Investigación y Desarrollo en Fermentaciones Industriales, CINDEFI. (UNLP; CCT-La Plata, CONICET). Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes.
  • Roque A. HOURS Centro de Investigación y Desarrollo en Fermentaciones Industriales, CINDEFI. (UNLP; CCT-La Plata, CONICET).
Keywords: pectin, protopectin, polysaccharide, Citrus limon.

Abstract

Pectin is a polysaccharide of vegetal origin widely used in food and pharmaceutical industries, among

others, due to its particular physicochemical properties. Pectin solubilizing activity of Protopectinase-

SE, an enzyme produced by the yeast-like fungus Geotrichum klebahnii, was studied using lemon albedo

(the inner white and spongy part of the peel) and protopectin (the water insoluble pectic substance

obtained from the same tissue) as substrates. Effects of substrate particle size and composition, pH and

temperature of reaction medium were evaluated using a Doehlert experimental design with response

surface. Temperature, pH and buffer (citric acid-sodium citrate, CitB) concentration display an important effect on pectin solubilization process, with optimal solubilization conditions at pH 5.0, 37°C and CitB

= 25 mM. Yield of 37 and 28 g of pectin/100 g tissue (dry basis) are achieved under optimal reaction

conditions from protopectin and albedo, respectively. In both cases, extracted pectin shows a 60% uronic

acid content and 78% esterification grade

|Abstract
= 15 veces | PDF (ESPAÑOL (ESPAÑA))
= 13 veces|

Downloads

Download data is not yet available.
Published
2009-05-11
How to Cite
ZAPATA Z. A. D., ESCOBAR G. C. A., CAVALLITO S. F., & HOURS R. A. (2009). EVALUATION OF PECTIN SOLUBILIZATION CAPABILITY FROM LEMON PEEL USING PROTOPECTINASE-SE. Vitae, 16(1). Retrieved from https://revistas.udea.edu.co/index.php/vitae/article/view/1427
Section
Biotechnology