• Hernán A. VALLE Z. Grupo de Investigación, Productos Naturales Marinos, Facultad de Química Farmacéutica, Universidad de Antioquia.
  • Gilmar G. SANTAFÉ P. Grupo de Investigación, Química de los Productos Naturales, Departamento de Química, Universidad de Córdoba.
Keywords: Mycale laevis, sponges, sterols, gas chromatography, mass spectrometry.


The development of marine bioprospection research requires to fortify of marine organisms inventories,

which also implies their exhaustive taxonomic classification. From the morphological point of view, the

classification of marine sponges is a complex procedure and it is facilitated with the use of chemotaxonomic

information based on the composition of its sterols. This study describes the free sterols composition of the

Mycale laevis sponge, collected in the Cispatá Bay (Córdoba, Colombia). The subextract of dichloromethane

is obtained from the methanol extract of the sponge; this subextract is fractionated through chromatography

in column on silica gel, using hexane-ethyl acetate mixtures with a gradual increase of the polarity as a

mobile phase. The obtention of the majoritary fraction (Rf = 0.77, silica gel /hexane: ethyl acetate 1:1) and its

analysis by means of gas chromatography coupled to mass spectrometry (GC-MS), allows the identification

of 9 monohydroxylated sterols with nuclei steroidal Δ0, Δ5 and Δ7 sterols. Stanols (nucleus Δ0) are the most

abundant in the sterolic fraction with 71.7% of the total of the present compounds. These results contribute

to chemotaxonomics studies marine sponges in the Mycalidae family.

= 17 veces | PDF (ESPAÑOL (ESPAÑA))
= 10 veces|


Download data is not yet available.
How to Cite
VALLE Z. H. A., & SANTAFÉ P. G. G. (2009). FREE STEROLS FROM THE MARINE SPONGE <I>Mycale laevis</I&gt;. Vitae, 16(1). Retrieved from
Natural Products