In vitro effect of terpenoid-rich extract of Momordica charantia on alpha glucosidase activity


  • AB SALLAU Ahumad Bello University Zaria
  • RN YAKUBU Ahumad Bello University Zaria
  • SM ALIYU Umaru Musa Yar’adua University
  • A SALIHU Ahumad Bello University Zaria
  • BY BONIFACE Ahumad Bello University Zaria



α-glucosidase, terpenoids, inhibitor, inhibition, hyperglycemia, extract.


Background: Momordica charantia is a vine, native to tropics and subtropics that has found use in herbal medicine. In Nigeria, the plant is locally used in the treatment of diabetes. Objective: To investigate the inhibitory effect of terpenoid-rich extract from Momordica charantia on α-glucosidase activity. Method: The inhibitory effect of the terpenoid-rich extract from Momordica charantia on the activity of alpha-glucosidase from Saccharomyces cerevisiae as well as the determination of the mode of inhibition were investigated using in vitro assay methods. Results: The results revealed that the terpenoid-rich extract had a high IC50 of 1.60 mg/mL. The Lineweaver-Burk plot revealed that the extract exhibited partially mixed–type inhibition of α-glucosidase. Conclusions: It can be inferred from this study that although terpenoid-rich extract inhibited α- glucosidase, further purification will be necessary on the extract so as to increase its potency as an inhibitor of α-glucosidase. The action of the extract on α-glucosidase provided an insight into the probable mechanism of action of Momordica charantia in reducing hyperglycemia.

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Author Biographies

AB SALLAU, Ahumad Bello University Zaria

Department of Biochemistry

RN YAKUBU, Ahumad Bello University Zaria

Department of Biochemistry

SM ALIYU, Umaru Musa Yar’adua University

Department of Biochemistry

A SALIHU, Ahumad Bello University Zaria

Department of Biochemistry

BY BONIFACE, Ahumad Bello University Zaria

Department of Biochemistry


Parkin CG, Brooks N. ¿Is Postprandial Glucose Control Important?. ¿Is It Practical In Primary Care Settings?. Clin Diabetes. 2002; 20:71-76.

Hanefeld M, Schmechel H, Julius U, Schwanebeck U. Determinants for coronary heart disease in non-insulin dependent diabetes mellitus: lessons from the diabetes inter vention study. Diabetes Res Clin Pract. 1996; 30(Suppl):67-70.

Hanefeld M, Fischer S, Julius U, Schulze J, Schwanebeck U, Schmechel H, et al. Risk factors for myocardial infarction and death in newly detected NIDDM: the Diabetes Intervention Study, 11-year follow-up. Diabetología. 1996; 39:1577-1583.

Ortiz-Andrade RR, García-Jiménez S, Castillo-España P, Ramírez-Ávila G, Villalobos-Molina R, Estrada-Soto S.

Alpha-Glucosidase inhibitory activity of the methanolic extract from Tournefortia hartwegiana: an antihyperglycemic agent. J Ethnopharmacol. 2007; 109:48-53.

Monnier L, Colette C. Contributions of fasting and postprandial glucose to hemoglobin A1c. Endocr Pract. 2006; 12(Suppl 1):42-46.

Sies H, Stahl W, Sevanian A. Nutritional, dietary and postprandial oxidative stress. J Nutr. 2005; 135:969-972.

Nichols BL, Avery SE, Karnsakul W, Jahoor F, Sen P, Swallow DM, et al. Congenital maltase-glucoamylase deficiency associated with lactase and sucrase deficiencies. J Pediatr Gastroenterol Nutr. 2002; 35:573-579.

Kwon YI, Apostolidis E, Shetty K. In vitro studies of eggplant (Solanum melongena) phenolics as inhibitors of key enzymes relevant for type 2 diabetes and hypertension. Bioresource Technol. 2008; 99: 2981-2988.

Joseph B, Raj SJ. Phytopharmacological properties of Ficus racemosa Linn - An overview. Int J Pharm Sci Rev Res. 2010b; 3(2):134-138.

Joseph B, Jini D, Ajisha SU. Phytochemical characterization of herbal drug formulation for arthritis. Res J Phytochem. 2012; 6(2):54-60.

Singh U, Singh S, Kochhar A. Therapeutic potential of antidiabetic neutraceuticals. Psychopharmacol. 2012; 2(1):144-169.

Zohary D, Hopf M. Domestication of plants in the old world. Oxford: Oxford University Press: 2000. 122 p.

Cefalu WT, Ye J, Wang ZQ. Efficacy of dietary supplementation with botanicals on carbohydrate metabolism in humans. Endocr Metab Immune Disord Drug Targets. 2008;8: 78-81. [PubMed]

Cousens G. There is a cure for diabetes: the tree of life 21-day program. California. North Atlantic Books: 2008. 191-192p.

Hasan I, Khatoon S. Effect of Momordica charantia (bitter gourd) tablets in diabetes mellitus: Type 1 and Type 2. Prime Res Med (PROM). 2012; 2(2):72-74

Bhushan MS, Rao CHV, Ojha SK, Vijayakumar M, Verma A. An analytical review of plants for anti-diabetic activity with their phytoconstituent and mechanism of action. IJPSR. 2010; 1(1):29-46.




How to Cite

SALLAU, A., YAKUBU, R., ALIYU, S., SALIHU, A., & BONIFACE, B. (2018). In vitro effect of terpenoid-rich extract of Momordica charantia on alpha glucosidase activity. Vitae, 25(3), 148–153.



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