In vitro effect of terpenoid-rich extract of Momordica charantia on alpha glucosidase activity

  • AB SALLAU Ahumad Bello University Zaria http://orcid.org/0000-0003-3715-8656
  • RN YAKUBU Ahumad Bello University Zaria
  • SM ALIYU Umaru Musa Yar’adua University, Katsina
  • A SALIHU Ahumad Bello University Zaria
  • BY BONIFACE Ahumad Bello University Zaria
Keywords: α-glucosidase, terpenoids, inhibitor, inhibition, hyperglycemia, extract.

Abstract

Background: Momordica charantia is a vine, native to tropics and subtropics that has found use in herbal medicine. In Nigeria, the plant is locally used in the treatment of diabetes. Objective: To investigate the inhibitory effect of terpenoid-rich extract from Momordica charantia on α-glucosidase activity. Method: The inhibitory effect of the terpenoid-rich extract from Momordica charantia on the activity of alpha-glucosidase from Saccharomyces cerevisiae as well as the determination of the mode of inhibition were investigated using in vitro assay methods. Results: The results revealed that the terpenoid-rich extract had a high IC50 of 1.60 mg/mL. The Lineweaver-Burk plot revealed that the extract exhibited partially mixed–type inhibition of α-glucosidase. Conclusions: It can be inferred from this study that although terpenoid-rich extract inhibited α- glucosidase, further purification will be necessary on the extract so as to increase its potency as an inhibitor of α-glucosidase. The action of the extract on α-glucosidase provided an insight into the probable mechanism of action of Momordica charantia in reducing hyperglycemia.
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Author Biographies

AB SALLAU, Ahumad Bello University Zaria
Department of Biochemistry
RN YAKUBU, Ahumad Bello University Zaria
Department of Biochemistry
SM ALIYU, Umaru Musa Yar’adua University, Katsina
Department of Biochemistry
A SALIHU, Ahumad Bello University Zaria
Department of Biochemistry
BY BONIFACE, Ahumad Bello University Zaria
Department of Biochemistry
Published
2018-12-18
How to Cite
SALLAU, A., YAKUBU, R., ALIYU, S., SALIHU, A., & BONIFACE, B. (2018). In vitro effect of terpenoid-rich extract of Momordica charantia on alpha glucosidase activity. Vitae, 25(3), 148-153. https://doi.org/10.17533/udea.vitae.v25n3a05
Section
Natural Products